Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,772 G→T intergenic (‑539/‑2135) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7720GT100.0% 477.2 / NA 130intergenic (‑539/‑2135)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (64/66);  total (64/66)

Only 100 of 134 total aligned reads displayed.

ATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAA  >  NC_000913_3_bme_pgi/4233628‑4233918
                                                                                                                                                |                                                                                                                                                   
ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGaa                                                                                                                                                >  2:1295877/1‑150 (MQ=255)
    aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                                                                                                            <  4:130094/150‑1 (MQ=255)
    aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCtt                                                                                                                                            <  3:431688/150‑1 (MQ=255)
      gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGa                                                                                                                                          >  3:289970/1‑150 (MQ=255)
       cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         <  3:508757/150‑1 (MQ=255)
       cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         <  1:807554/150‑1 (MQ=255)
       cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         <  1:807221/150‑1 (MQ=255)
            cTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAAc                                                                                                                                    <  1:783566/150‑1 (MQ=255)
             tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACg                                                                                                                                   >  1:57217/1‑150 (MQ=255)
              gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACgg                                                                                                                                  >  4:605271/1‑150 (MQ=255)
              gcgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACgg                                                                                                                                  <  3:605271/150‑1 (MQ=255)
                gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAACGGATAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTc                                                                                                                                >  3:17100/1‑150 (MQ=255)
                 cTAAGGGTTTACACTCAACATTACGCTAACGGCACCAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCg                                                                                                                               >  1:1402722/1‑150 (MQ=255)
                  tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGc                                                                                                                              <  3:577791/150‑1 (MQ=255)
                     gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAGGCAACGGTCGCaaa                                                                                                                           <  4:427237/150‑1 (MQ=255)
                     gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaaa                                                                                                                           <  4:427143/150‑1 (MQ=255)
                        tttACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGTCGCTACGAAGCTTGAAGCAACGGTAGCAAAGCt                                                                                                                        <  2:482527/150‑1 (MQ=255)
                        tttACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCt                                                                                                                        <  3:381215/150‑1 (MQ=255)
                           acacTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaa                                                                                                                     <  4:426300/150‑1 (MQ=255)
                              cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         >  2:37180/1‑127 (MQ=255)
                              cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         <  1:37180/127‑1 (MQ=255)
                              cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                                  >  2:852844/1‑150 (MQ=255)
                               tataCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                                 <  3:479619/147‑1 (MQ=255)
                                    aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                            >  1:491194/1‑150 (MQ=255)
                                      tACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTggg                                                                                                          <  2:546035/150‑1 (MQ=255)
                                            aaCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAAt                                                                                                    >  3:37052/1‑150 (MQ=255)
                                            aaCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt                                                                                                    >  1:503310/1‑150 (MQ=255)
                                             aCGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                                   >  1:1297783/1‑150 (MQ=255)
                                              cGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                                  >  3:249734/1‑150 (MQ=255)
                                               ggCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                                 >  3:100311/1‑150 (MQ=255)
                                                    tAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                                  >  2:678896/1‑144 (MQ=255)
                                                    tAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                                  <  1:678896/144‑1 (MQ=255)
                                                       aaCCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                                         <  4:249734/150‑1 (MQ=255)
                                                         ccATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa                                                                                      <  1:926250/150‑1 (MQ=255)
                                                          cATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                     <  3:75581/150‑1 (MQ=255)
                                                           attacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata                                                                                    <  4:117171/147‑1 (MQ=255)
                                                                aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                     >  2:447412/1‑129 (MQ=255)
                                                                aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                     <  1:447412/129‑1 (MQ=255)
                                                                 tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg                                                                              <  4:551826/150‑1 (MQ=255)
                                                                  ttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc                                                                             <  2:1402722/150‑1 (MQ=255)
                                                                   tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGcgcgcg                                                                            <  3:411658/150‑1 (MQ=255)
                                                                         tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                             <  1:532050/112‑1 (MQ=255)
                                                                               gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGcc                                                                >  3:178394/1‑150 (MQ=255)
                                                                                      aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                                         <  2:785744/119‑1 (MQ=255)
                                                                                      aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCccg                                                         <  2:491194/150‑1 (MQ=255)
                                                                                        tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                       <  3:917428/150‑1 (MQ=255)
                                                                                        tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                       >  2:591296/1‑150 (MQ=255)
                                                                                          ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggccg                                                     >  1:1371726/1‑150 (MQ=255)
                                                                                             cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                  <  2:57217/150‑1 (MQ=255)
                                                                                              aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                 >  2:203843/1‑150 (MQ=255)
                                                                                               aaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCg                                                >  3:315627/1‑150 (MQ=255)
                                                                                                aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                               <  4:178394/150‑1 (MQ=255)
                                                                                                 gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                              <  1:1043273/150‑1 (MQ=255)
                                                                                                      aaTTCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccg                                                         >  2:1351986/1‑134 (MQ=255)
                                                                                                        ttCTCAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGTGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATAtt                                       <  2:1371726/150‑1 (MQ=255)
                                                                                                         tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                                        >  1:1082470/1‑85 (MQ=255)
                                                                                                         tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                                        <  2:1082470/85‑1 (MQ=255)
                                                                                                         tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                                         <  3:784665/115‑1 (MQ=255)
                                                                                                         tctcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                                         >  4:784665/1‑115 (MQ=255)
                                                                                                           tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         >  3:718196/1‑50 (MQ=255)
                                                                                                           tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGaa                                                                                                                                         <  4:718196/50‑1 (MQ=255)
                                                                                                           tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATATcgcgcg                                                                            >  4:832299/1‑110 (MQ=255)
                                                                                                           tcAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc                                    <  4:231482/150‑1 (MQ=255)
                                                                                                            cAAAATCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                       <  1:1309915/130‑1 (MQ=255)
                                                                                                             aaaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCACAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctc                                  >  3:552295/1‑150 (MQ=255)
                                                                                                              aaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                          >  3:3550/1‑94 (MQ=255)
                                                                                                              aaaTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                          <  4:3550/94‑1 (MQ=255)
                                                                                                              aaaTCAGAAGAGTACTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                                 >  2:673947/1‑150 (MQ=255)
                                                                                                                aTCAGAAGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGGATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                               <  2:909160/150‑1 (MQ=255)
                                                                                                                    gaagaGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                <  4:311992/97‑1 (MQ=255)
                                                                                                                    gaagaGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                >  3:311992/1‑97 (MQ=255)
                                                                                                                         gaaTTGCTAATGGAAAGACACGCTCCGAAACTTGCAGCATCGGTCCCAACGTCGTTGAAGCTTTCGGCGGTATCGCCCCCCCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat                      <  3:584514/148‑1 (MQ=255)
                                                                                                                          tATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                                         >  4:487971/1‑83 (MQ=255)
                                                                                                                           aTTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTCGATGAtcg                    >  4:772366/1‑149 (MQ=255)
                                                                                                                            ttGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   >  4:565843/1‑150 (MQ=255)
                                                                                                                            ttGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   >  2:331077/1‑150 (MQ=255)
                                                                                                                                tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                               <  1:486632/118‑1 (MQ=255)
                                                                                                                                tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc                                      <  1:1136514/127‑1 (MQ=255)
                                                                                                                                tAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc                                      >  2:1136514/1‑127 (MQ=255)
                                                                                                                                 aaTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt              >  2:528296/1‑150 (MQ=255)
                                                                                                                                 aaTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt              <  1:331077/150‑1 (MQ=255)
                                                                                                                                  aTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                               >  1:549651/1‑69 (MQ=255)
                                                                                                                                  aTGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                               <  2:549651/69‑1 (MQ=255)
                                                                                                                                   tGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGAGATTCGCTCTGCCTGGATGATCTTCTGTTCg            >  1:902915/1‑150 (MQ=255)
                                                                                                                                    ggaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCTATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCGTGGATGATCTTCTGTTCGa           >  2:1570183/4‑150 (MQ=255)
                                                                                                                                    ggaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGGTATTCGCTCTGCCTGGATGATCTTCTGTTCGa           <  3:524768/147‑1 (MQ=255)
                                                                                                                                    ggaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa           <  2:509913/147‑1 (MQ=255)
                                                                                                                                    ggaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa           <  1:835385/147‑1 (MQ=255)
                                                                                                                                     gaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGata                                         <  4:504050/117‑1 (MQ=255)
                                                                                                                                     gaaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGata                                         >  3:504050/3‑119 (MQ=255)
                                                                                                                                      aaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   >  2:999782/2‑140 (MQ=255)
                                                                                                                                      aaaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   <  1:999782/139‑1 (MQ=255)
                                                                                                                                        agagACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTgg       >  4:305853/1‑150 (MQ=255)
                                                                                                                                           gaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGATAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTg                >  4:768461/1‑138 (MQ=255)
                                                                                                                                           gaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGATAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTg                <  3:768461/138‑1 (MQ=255)
                                                                                                                                            aCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGa   >  3:786266/1‑150 (MQ=255)
                                                                                                                                            aCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGa   <  1:1295877/150‑1 (MQ=255)
                                                                                                                                             cgctACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa  <  1:698624/150‑1 (MQ=255)
                                                                                                                                             cgctACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa  >  1:407304/1‑150 (MQ=255)
                                                                                                                                             cgctACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa  >  4:834123/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAA  >  NC_000913_3_bme_pgi/4233628‑4233918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: