Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 722,162 C→A M751I (ATG→ATT kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi722,1620CA96.4% 391.9 / 5.4 114M751I (ATG→ATTkdpDfused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (53/55);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 115 total aligned reads displayed.

ATACCAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCGCTGGCCT  >  NC_000913_3_bme_pgi/722013‑722307
                                                                                                                                                     |                                                                                                                                                 
aTACCAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGc                                                                                                                                                   >  1:1354891/1‑150 (MQ=255)
   ccAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAAtt                                                                                                                                                <  3:639261/150‑1 (MQ=255)
    cAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAAttt                                                                                                                                               >  2:585642/1‑150 (MQ=255)
    cAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGAAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAAttt                                                                                                                                               >  3:501984/1‑150 (MQ=255)
     aaTTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTg                                                                                                                                              <  1:585642/150‑1 (MQ=255)
       tttCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCa                                                                                                                                            >  1:531885/1‑150 (MQ=255)
       tttCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCa                                                                                                                                            <  2:1267647/150‑1 (MQ=255)
       tttCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCa                                                                                                                                            <  4:846721/150‑1 (MQ=255)
        ttCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAg                                                                                                                                           <  1:1582115/150‑1 (MQ=255)
        ttCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAg                                                                                                                                           <  4:501984/150‑1 (MQ=255)
            gCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGCTTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAgcgcg                                                                                                                                       >  2:1184565/1‑150 (MQ=255)
            gCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAgcgcg                                                                                                                                       <  1:1289969/150‑1 (MQ=255)
                   cACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGa                                                                                                                                <  2:1110377/150‑1 (MQ=255)
                   cACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGa                                                                                                                                <  2:249105/150‑1 (MQ=255)
                   cACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGACAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGa                                                                                                                                <  2:720355/150‑1 (MQ=255)
                     cccGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGact                                                                                                                              <  4:324035/150‑1 (MQ=255)
                            atTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTActtctt                                                                                                                       <  4:138461/150‑1 (MQ=255)
                               tCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCa                                                                                                                    >  3:51331/1‑150 (MQ=255)
                                cACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAg                                                                                                                   <  2:317783/150‑1 (MQ=255)
                                 aCCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGc                                                                                                                  <  3:90365/150‑1 (MQ=255)
                                  ccGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGGAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTg                                                                                                                                              >  4:838561/1‑121 (MQ=255)
                                  ccGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTg                                                                                                                                              <  3:838561/121‑1 (MQ=255)
                                      gTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTaa                                                                                                             <  3:119250/150‑1 (MQ=255)
                                       ttCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            <  2:1084043/150‑1 (MQ=255)
                                       ttCTCCAGCAGATTAATCAGCAACCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACCATAACCCCGCTCCCAGAATATGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            <  2:1590429/150‑1 (MQ=255)
                                           ccAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCAct                                                                                                        >  2:1055121/1‑150 (MQ=255)
                                            cagcagATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTActtc                                                                                                                         <  4:405864/132‑1 (MQ=255)
                                             agcagATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAGGGTCAGCGGTTCTGGCGGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCActct                                                                                                      >  3:930049/1‑150 (MQ=255)
                                               cagATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtcttt                                                                                                    >  2:782546/1‑150 (MQ=255)
                                                agATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtctttc                                                                                                   <  3:224150/150‑1 (MQ=255)
                                                       tCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            >  1:993314/1‑134 (MQ=255)
                                                       tCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            <  2:993314/134‑1 (MQ=255)
                                                         aGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTaa                                                                                                             >  1:1523282/1‑131 (MQ=255)
                                                         aGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTaa                                                                                                             <  2:1523282/131‑1 (MQ=255)
                                                          gCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAg                                                                                                                                           >  1:86835/1‑100 (MQ=255)
                                                          gCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAg                                                                                                                                           <  2:86835/100‑1 (MQ=255)
                                                          gCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGa                                                                                                                                >  4:708023/1‑111 (MQ=255)
                                                              ccGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgcc                                                                                     >  1:94619/1‑150 (MQ=255)
                                                              ccGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgcc                                                                                     >  2:1431321/1‑150 (MQ=255)
                                                               cGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGACGACGATGCACCCGGTGCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgccg                                                                                    >  1:1349601/1‑150 (MQ=255)
                                                                  tCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGccgccg                                                                                 <  2:94619/150‑1 (MQ=255)
                                                                    aaaGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGa                                                                               >  3:453719/1‑150 (MQ=255)
                                                                    aaaGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGa                                                                               >  1:987208/1‑150 (MQ=255)
                                                                      agagTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTcc                                                                                                                     <  1:869175/110‑1 (MQ=255)
                                                                      agagTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTcc                                                                                                                     >  2:869175/1‑110 (MQ=255)
                                                                      agacTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATGGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTACAGCCGCCGGACt                                                                             <  2:1387166/150‑1 (MQ=255)
                                                                        agTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCGGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGa                                                                           <  4:930049/150‑1 (MQ=255)
                                                                         gTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGaa                                                                          <  3:842224/150‑1 (MQ=255)
                                                                            gCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTc                                                                       >  1:252245/1‑150 (MQ=255)
                                                                              ccGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTcgc                                                                     <  1:527245/150‑1 (MQ=255)
                                                                              ccGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTcgc                                                                     >  4:398047/1‑150 (MQ=255)
                                                                              ccGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTcgc                                                                     <  3:505412/150‑1 (MQ=255)
                                                                              ccGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTcgc                                                                     <  2:531885/150‑1 (MQ=255)
                                                                               cGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTcgcg                                                                    <  1:686426/150‑1 (MQ=255)
                                                                                gTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgccgc                                                                                   >  4:169708/1‑134 (MQ=255)
                                                                                gTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgccgc                                                                                   <  3:169708/134‑1 (MQ=255)
                                                                                gTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCgcgc                                                                   <  4:760496/150‑1 (MQ=255)
                                                                                   aaCGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            >  1:1171355/1‑106 (MQ=255)
                                                                                   aaCGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            <  2:1171355/106‑1 (MQ=255)
                                                                                        ggATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTg                                                                                                                                    >  1:1025278/1‑77 (MQ=255)
                                                                                        ggATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTg                                                                                                                                    <  2:1025278/77‑1 (MQ=255)
                                                                                         gATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAg                                                          <  1:1431321/150‑1 (MQ=255)
                                                                                          aTTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtctttct                                                                                                  >  1:1579501/1‑109 (MQ=255)
                                                                                          aTTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtctttct                                                                                                  <  2:1579501/109‑1 (MQ=255)
                                                                                           ttAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            >  2:852817/1‑98 (MQ=255)
                                                                                           ttAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc                                                                                                            <  1:852817/98‑1 (MQ=255)
                                                                                           ttAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTa                                                        <  4:849434/150‑1 (MQ=255)
                                                                                           ttAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTa                                                        >  3:849434/1‑150 (MQ=255)
                                                                                           ttAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATACAGTa                                                        >  4:794181/1‑150 (MQ=255)
                                                                                              aGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGat                                                     >  4:746302/1‑150 (MQ=255)
                                                                                              aGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGat                                                     >  1:1077667/1‑150 (MQ=255)
                                                                                                gTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGatta                                                   >  1:38331/1‑150 (MQ=255)
                                                                                                gTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGatta                                                   >  1:841992/1‑150 (MQ=255)
                                                                                                   aGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTc                                                >  2:1542980/1‑150 (MQ=255)
                                                                                                   aGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTc                                                >  4:190906/1‑150 (MQ=255)
                                                                                                     cttttCTGGCAGAGAATGATTGTTGGTCTACGTTATACTCGGTTCCAGTATTTGCTGCGCGCTGCCGACTACTTCTTCTGGCGTTAACCACTCTTTCTTCATATTAAATCCGCCGCACTGAATTCGCGCCATATCCAGTAGATTATTCAc                                              <  4:516664/147‑1 (MQ=255)
                                                                                                     cGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCAc                                              <  4:453719/150‑1 (MQ=255)
                                                                                                            ggCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGa                                                                               >  3:529053/1‑110 (MQ=255)
                                                                                                            ggCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGa                                                                               <  4:529053/110‑1 (MQ=255)
                                                                                                             gCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCggg                                      >  4:899391/1‑150 (MQ=255)
                                                                                                               agagAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCAc                                              <  4:304878/140‑1 (MQ=255)
                                                                                                               agagAAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCAc                                              >  3:304878/1‑140 (MQ=255)
                                                                                                                 agaAAGATTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAgt                                  >  2:765818/1‑150 (MQ=255)
                                                                                                                       aTTGATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAg                            <  3:398047/150‑1 (MQ=255)
                                                                                                                          gatgaccgacgaTAAAGCCAGAGCCAGTATTTGCATCGTACAGCCGACTACTTCTTACAGCGTAAACCACTCTTTCTTCTAATAATAGCCGCCCTGCTGAATTCACTACCAATCCACTTTATTATTCACCAGTCGGGTGGTGTTCAGcac                         >  4:225851/7‑150 (MQ=255)
                                                                                                                          gATGGGCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGCTTATTCACCAGTCGGGTAGTGTTCAGcac                         >  4:786568/1‑150 (MQ=255)
                                                                                                                             gggCGACGATAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGattatt                                                 <  1:1007769/123‑1 (MQ=255)
                                                                                                                                cgacgaTAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTg                   >  4:372241/1‑150 (MQ=255)
                                                                                                                                      tAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGTATTCGCGCCATATCCATTAGATTATTCACCAGTCGGGGAGTGTTCAGCACATGCTGACGGAt             >  1:1510235/1‑150 (MQ=255)
                                                                                                                                      tAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGatta                                                   <  4:784737/112‑1 (MQ=255)
                                                                                                                                      tAAACCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGatta                                                   >  3:784737/1‑112 (MQ=255)
                                                                                                                                        aaCCCGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGAtct           >  2:325733/1‑150 (MQ=255)
                                                                                                                                          cccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCg         <  3:746302/150‑1 (MQ=255)
                                                                                                                                           ccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGccgccg                                                                                 >  1:482908/1‑77 (MQ=255)
                                                                                                                                           ccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGccgccg                                                                                 <  2:482908/77‑1 (MQ=255)
                                                                                                                                           ccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGcac                         >  1:522392/1‑133 (MQ=255)
                                                                                                                                           ccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGcac                         <  2:522392/133‑1 (MQ=255)
                                                                                                                                           ccGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCGc        >  3:497714/1‑150 (MQ=255)
                                                                                                                                            cGGTTCCAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCgtt       <  4:121253/150‑3 (MQ=255)
                                                                                                                                                 ccAGAATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCGatggcct  >  4:274072/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATACCAATTTCGGCCTGCGCACCCGCATATTTCACCGCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATATCCAGTAGATTATTCACCAGTCGGGTAGTGTTCAGCACATGCTGACGGATCTCGCTGGCCT  >  NC_000913_3_bme_pgi/722013‑722307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: