BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,233,772	G→T	intergenic (‑539/‑2135)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,233,772	0	G	T	100.0%	529.3 / NA	146	intergenic (‑539/‑2135)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (80/66); total (80/66)

Only 100 of 149 total aligned reads displayed.

AATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAA  >  NC_000913_3_bme_pgi/4233627‑4233918

 ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGaa                                                                                                                                               >  2:641824/1‑150 (MQ=255)

  taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAg                                                                                                                                              <  2:268503/150‑1 (MQ=255)

  taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAg                                                                                                                                              <  2:268763/150‑1 (MQ=255)

   aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGc                                                                                                                                             >  4:532140/1‑150 (MQ=255)

   aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGc                                                                                                                                             >  1:1626552/1‑150 (MQ=255)

      tGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTg                                                                                                                                          <  2:845416/150‑1 (MQ=255)

         ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCAGATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTGCGAAGCTTGAAg                                                                                                                                       >  2:1063551/1‑150 (MQ=255)

              tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACg                                                                                                                                  >  2:734603/1‑150 (MQ=255)

                cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCACGAGACGCTACGAAGCTTGAAGCAACGGt                                                                                                                                >  3:613584/1‑150 (MQ=255)

                  cTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGTGACGCTACGAAGCTTGAAGCAACGGTCg                                                                                                                              <  2:427980/150‑1 (MQ=255)

                   tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCaa                                                                                                                                    >  1:1086364/1‑143 (MQ=255)

                   tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCaa                                                                                                                                    <  2:1086364/143‑1 (MQ=255)

                   tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGc                                                                                                                             >  4:782724/1‑150 (MQ=255)

                      gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaaa                                                                                                                          <  2:1229846/150‑1 (MQ=255)

                       ggTTTACACTCAATATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGGATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                         <  2:1040257/150‑1 (MQ=255)

                        gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                                                        <  3:120617/150‑1 (MQ=255)

                          ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTGCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTg                                                                                                                      >  4:284451/1‑150 (MQ=255)

                               cTCAACATTACGCTAACGGCACTAAAACCACCACATTTTTCTGTGACTGGCGCTGCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                                 <  3:284451/150‑1 (MQ=255)

                                     aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                           >  3:322528/1‑150 (MQ=255)

                                      ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                                                                                                          >  1:1497136/1‑150 (MQ=255)

                                          gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAATGTTACAATTCTCACAATCAGAAGATTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                                                                                                      <  1:619241/150‑1 (MQ=255)

                                          gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCtt                                                                                                                                           >  2:556203/1‑113 (MQ=255)

                                          gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCtt                                                                                                                                           <  1:556203/113‑1 (MQ=255)

                                          gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                                                                                                      >  1:1770325/1‑150 (MQ=255)

                                            tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                    >  3:811954/1‑150 (MQ=255)

                                            tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                    <  1:641800/150‑1 (MQ=255)

                                             aaCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt                                                                                                   <  3:478686/150‑1 (MQ=255)

                                                 gCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGc                                                                                               >  1:402149/1‑150 (MQ=255)

                                                   aCTAAAACCATCACATTTTTCTGTGACTGGCGCCACAATATTCCCAAGACGCCATTCTAAACACCGGGCGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGccc                                                                                             <  1:1063551/150‑1 (MQ=255)

                                                    cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                                                                                            >  4:138721/1‑150 (MQ=255)

                                                    cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                                                                                            >  2:340064/1‑150 (MQ=255)

                                                             tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat                                                                                   >  1:613629/1‑150 (MQ=255)

                                                                caTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                >  1:578106/1‑150 (MQ=255)

                                                                caTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                >  1:1567689/1‑150 (MQ=255)

                                                                caTTTTTCTGTGACTGGCGCTACAATCCTCCAAAGACACAATCCTCAACATCCGCAGAGTATTGCCAATGGCAAGAGACGCTACCAAGCTTGACGCAACGGTCCCAAAAATAAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                <  4:483347/150‑1 (MQ=255)

                                                                 aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc                                                                                                               <  1:1716629/118‑1 (MQ=255)

                                                                 aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc                                                                                                               >  2:1716629/1‑118 (MQ=255)

                                                                 aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                     <  1:430720/144‑1 (MQ=255)

                                                                 aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                     >  2:430720/1‑144 (MQ=255)

                                                                  tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg                                                                              >  4:78014/1‑150 (MQ=255)

                                                                  tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg                                                                              >  1:105962/1‑150 (MQ=255)

                                                                   tttgcggtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCTCGCGCGATATgcgcgc                                                                             <  2:932479/144‑1 (MQ=255)

                                                                       cTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCGCGCGC‑ATAT‑CGCGCggggcc                                                                       <  1:1243023/150‑5 (MQ=255)

                                                                         gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAtt                                                                       <  4:573834/150‑1 (MQ=255)

                                                                             cTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgc                                                                   <  2:500947/150‑1 (MQ=255)

                                                                                 cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                                                        >  3:973522/1‑93 (MQ=255)

                                                                                 cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGAAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCg                                                               >  1:1337415/1‑150 (MQ=255)

                                                                                    tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCCAGAGACGCTACGAAGCTTGAAGCAACGGGCGCAAAGATGAAAAAGGAGTTGGCGGAAACGGCCCCGCGCGAAATGCGCGCGGCATTCAGCGCCGAcc                                                            >  1:1621221/1‑150 (MQ=255)

                                                                                         tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGCATTGCTAATGGCAAGAGGCGCTACGAAGCTTGAAGCAACAGACGCAAAGCTCAAAAAACATTGGGCAGAATCGGCCCCGCGCGATATGCGCACCGCATTCAGCACCGACCccggc                                                       >  2:173865/1‑150 (MQ=255)

                                                                                            tCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggccgg                                                    >  4:87268/1‑150 (MQ=255)

                                                                                             ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                                 >  2:78526/1‑88 (MQ=255)

                                                                                             ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                                 <  1:78526/88‑1 (MQ=255)

                                                                                             ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                                         >  3:682306/1‑128 (MQ=255)

                                                                                             ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCa                                                                         <  4:682306/128‑1 (MQ=255)

                                                                                             ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGt                                                   >  3:203131/1‑150 (MQ=255)

                                                                                              cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                  <  3:97924/150‑1 (MQ=255)

                                                                                               aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGa                                                                                                                                         <  2:1683805/62‑1 (MQ=255)

                                                                                               aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGa                                                                                                                                         >  1:1683805/1‑62 (MQ=255)

                                                                                               aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                 <  4:946341/150‑1 (MQ=255)

                                                                                               aaaGTCACAATTCTCAAAATCAGAAGAGCATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                 >  2:384278/1‑150 (MQ=255)

                                                                                                  gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                              <  4:443766/150‑1 (MQ=255)

                                                                                                  gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                              <  1:384278/150‑1 (MQ=255)

                                                                                                   tCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                                             <  3:450089/150‑1 (MQ=255)

                                                                                                    cacaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg                                            <  1:231160/150‑1 (MQ=255)

                                                                                                      caATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                       >  2:255069/1‑137 (MQ=255)

                                                                                                      caATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCg                                                >  2:1610814/1‑144 (MQ=255)

                                                                                                       aaTTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGatc                                         >  2:764665/1‑149 (MQ=255)

                                                                                                       aaTTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGaca                                         >  2:1526778/1‑148 (MQ=255)

                                                                                                           ctcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                         >  1:263129/1‑98 (MQ=255)

                                                                                                            tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc                                    >  1:1787375/1‑150 (MQ=255)

                                                                                                            tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc                                    >  1:515644/1‑150 (MQ=255)

                                                                                                             cAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGct                                   >  4:261738/1‑150 (MQ=255)

                                                                                                              aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATg                                                                                  <  1:465517/102‑1 (MQ=255)

                                                                                                                aaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTg                                >  1:655065/1‑150 (MQ=255)

                                                                                                                 aTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                               <  3:1044112/150‑1 (MQ=255)

                                                                                                                  tCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                                                                                                          <  4:1101135/74‑1 (MQ=255)

                                                                                                                   cAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCt                             <  2:1770325/150‑1 (MQ=255)

                                                                                                                     gaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTgg                           <  1:299208/150‑1 (MQ=255)

                                                                                                                      aagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGga                          <  1:640475/150‑1 (MQ=255)

                                                                                                                       agagTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgat                         <  2:1132629/150‑1 (MQ=255)

                                                                                                                         agTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                         >  2:1405170/1‑52 (MQ=255)

                                                                                                                             ttGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   <  1:734603/150‑1 (MQ=255)

                                                                                                                              tGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttc                  >  3:804701/1‑150 (MQ=255)

                                                                                                                                cTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                   <  2:603532/147‑1 (MQ=255)

                                                                                                                                 tAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGa                                                              >  4:664285/1‑103 (MQ=255)

                                                                                                                                 tAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCg                                                >  3:698852/1‑117 (MQ=255)

                                                                                                                                   aTGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCt                             >  3:603164/1‑134 (MQ=255)

                                                                                                                                   aTGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTc             >  2:1732980/1‑150 (MQ=255)

                                                                                                                                    tGGCAAGAGACGCTACGAAGCTTGAAGTAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCg            >  2:1003156/1‑150 (MQ=255)

                                                                                                                                    tGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCg            >  3:341471/1‑150 (MQ=255)

                                                                                                                                     ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                                             <  2:580993/116‑1 (MQ=255)

                                                                                                                                     ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                                             >  1:580993/1‑116 (MQ=255)

                                                                                                                                     ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa           >  2:1419865/1‑150 (MQ=255)

                                                                                                                                       cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                 <  1:810872/110‑1 (MQ=255)

                                                                                                                                       cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                 >  2:810872/1‑110 (MQ=255)

                                                                                                                                       cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACt         >  3:408212/1‑150 (MQ=255)

                                                                                                                                        aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGcc                                                                <  1:241367/94‑1 (MQ=255)

                                                                                                                                        aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGcc                                                                >  2:241367/1‑94 (MQ=255)

                                                                                                                                          gagaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt      >  1:658741/1‑150 (MQ=255)

                                                                                                                                              cgctACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGTGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACCAGTCGaa  <  1:927024/150‑1 (MQ=255)

AATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAA  >  NC_000913_3_bme_pgi/4233627‑4233918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`