Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,772 G→T intergenic (‑539/‑2135) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7720GT99.1% 806.0 / NA 222intergenic (‑539/‑2135)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (122/98);  minor base A (1/1);  total (123/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGA  >  NC_000913_3_bme_pgi/4233625‑4233917
                                                                                                                                                   |                                                                                                                                                  
  aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTTTTTTTTTTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGctacga                                                                                                                                                >  4:1272713/1‑150 (MQ=255)
  aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGctacga                                                                                                                                                >  4:541195/1‑150 (MQ=255)
       aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCtt                                                                                                                                           <  1:1103205/150‑1 (MQ=255)
       aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCtt                                                                                                                                           >  1:1886244/1‑150 (MQ=255)
         gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGa                                                                                                                                         >  2:524944/1‑150 (MQ=255)
           ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAg                                                                                                                                       >  1:311918/1‑150 (MQ=255)
           ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAg                                                                                                                                       >  2:723454/1‑150 (MQ=255)
           ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAg                                                                                                                                       <  1:1945454/150‑1 (MQ=255)
                tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACg                                                                                                                                  >  2:1942066/1‑150 (MQ=255)
                        gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaaa                                                                                                                          <  2:2453950/150‑1 (MQ=255)
                         ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                         >  3:769729/1‑150 (MQ=255)
                         ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                         <  2:856536/150‑1 (MQ=255)
                         ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGGCGCAAAg                                                                                                                         >  1:1983762/1‑150 (MQ=255)
                           tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAAc                                                                                                                                   >  2:1594700/1‑138 (MQ=255)
                           tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCt                                                                                                                       >  1:644697/1‑150 (MQ=255)
                             tACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGa                                                                                                                     >  1:1777678/1‑150 (MQ=255)
                               cacTCAACATTACGCTAACGGCACTAAAACCATCACATATTTCTGTGACTGGTGCTACAATCTCCCAAAGTCACAATTCTCAACATCAGAAGAGAATTGCTAATGGCACGAGACGCTACGAAGCTTGAAAGCACGGTCGCAAAGCTGaaa                                                                                                                   >  2:2509758/1‑150 (MQ=255)
                                acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACACTTATAAAAATACAAAGAGTCAAGCAACTAGGAAGAGACGCAAAGAAGATTGAAGAAAAGGTCGCAAAGCTGaaaa                                                                                                                  >  1:373752/1‑150 (MQ=255)
                                      cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                            <  1:1288149/150‑1 (MQ=255)
                                        ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTTACTGGTTCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                                                                                                          >  2:787277/1‑150 (MQ=255)
                                        ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                                                                                                          >  4:533896/1‑150 (MQ=255)
                                         tACGCTAACGGCACTAAAACCATCACATTTTTTTTTTTTTTTTTCTACAATCTTCCCAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCACGAGACTCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTggg                                                                                                         >  2:451397/1‑150 (MQ=255)
                                           cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                                       >  1:2031645/1‑150 (MQ=255)
                                            gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                                                                                                      >  4:213208/1‑150 (MQ=255)
                                              tAACGGCACTAAAACCATCACATTTTTTTTTTTTTTTTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                    >  4:1301104/1‑150 (MQ=255)
                                              tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                                                                                                    >  2:2110336/1‑150 (MQ=255)
                                                  ggCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                                >  3:367211/1‑150 (MQ=255)
                                                    cACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                              >  2:190209/1‑150 (MQ=255)
                                                     aCTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTCCAAAAGTACCAATTCACACACTCAGAAGAGAAACGCCTATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGccc                                                                                             <  3:146061/150‑1 (MQ=255)
                                                      cTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                                                                                            <  1:1094942/150‑1 (MQ=255)
                                                         aaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg                                                                                         >  3:1024878/1‑150 (MQ=255)
                                                          aaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc                                                                                        >  1:1214287/1‑150 (MQ=255)
                                                             cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                    <  3:1244281/150‑1 (MQ=255)
                                                             cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                    >  3:452275/1‑150 (MQ=255)
                                                             cATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                    <  3:615248/150‑1 (MQ=255)
                                                              aTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata                                                                                   <  1:906035/150‑1 (MQ=255)
                                                                     ttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc                                                                            >  2:2339467/1‑150 (MQ=255)
                                                                     ttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCACGCGATATgcgcgc                                                                            >  4:1228884/1‑150 (MQ=255)
                                                                       ttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCATATCGCGCgggg                                                                        <  2:553657/150‑3 (MQ=255)
                                                                         cTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCGCGCGCATATCGCGCggggcc                                                                      <  1:265354/150‑5 (MQ=255)
                                                                          tgtgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAt                                                                       >  2:1436939/1‑150 (MQ=255)
                                                                           gtgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAtt                                                                      <  1:515969/150‑1 (MQ=255)
                                                                            tgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCaa                                                                                                                                    <  1:2243544/88‑1 (MQ=255)
                                                                            tgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCaa                                                                                                                                    >  2:2243544/1‑88 (MQ=255)
                                                                            tgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTc                                                                     <  2:701518/150‑1 (MQ=255)
                                                                                tggtgcTACAATCTTACAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgcg                                                                 <  1:787277/146‑1 (MQ=255)
                                                                                  gtgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                         >  4:308587/3‑93 (MQ=255)
                                                                                  gtgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGcc                                                               >  1:716447/3‑150 (MQ=255)
                                                                                    gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGa                                                             >  3:1536133/1‑150 (MQ=255)
                                                                                     cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                                  >  1:753588/1‑113 (MQ=255)
                                                                                     cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                                  <  2:753588/113‑1 (MQ=255)
                                                                                      tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGc                                                                         <  1:1782334/136‑1 (MQ=255)
                                                                                      tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGc                                                                         >  2:1782334/1‑136 (MQ=255)
                                                                                         aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccg                                                        >  2:1359832/1‑150 (MQ=255)
                                                                                         aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccg                                                        >  1:1404090/1‑150 (MQ=255)
                                                                                                cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                 <  4:1110940/150‑1 (MQ=255)
                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                <  2:908905/150‑1 (MQ=255)
                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                                <  2:312692/150‑1 (MQ=255)
                                                                                                   aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                                >  1:1622215/1‑101 (MQ=255)
                                                                                                   aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                              <  2:1088953/150‑1 (MQ=255)
                                                                                                     tCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                                            <  3:257206/150‑1 (MQ=255)
                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTggg                                                                                                         <  1:1584987/88‑1 (MQ=255)
                                                                                                        caATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGat                                         >  1:2463032/1‑150 (MQ=255)
                                                                                                           ttCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATAtt                                      <  4:850644/150‑1 (MQ=255)
                                                                                                             ctcAAAATCAGAAGAGTAGTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCg                                    <  1:2509758/150‑1 (MQ=255)
                                                                                                              tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAtt                                                                      <  1:2281810/115‑1 (MQ=255)
                                                                                                                   aTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                              <  2:844041/150‑1 (MQ=255)
                                                                                                                      agaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgcgc                                                                <  3:1432873/113‑1 (MQ=255)
                                                                                                                      agaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgcgc                                                                >  4:1432873/1‑113 (MQ=255)
                                                                                                                      agaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggcc                                                     >  1:2046698/1‑124 (MQ=255)
                                                                                                                      agaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCg                                               <  3:727210/130‑1 (MQ=255)
                                                                                                                       gaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGc                                                                         >  1:2148434/1‑103 (MQ=255)
                                                                                                                       gaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGc                                                                         <  2:2148434/103‑1 (MQ=255)
                                                                                                                       gaagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGTATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTgg                          <  2:1579586/150‑1 (MQ=255)
                                                                                                                        aagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGga                         <  2:1214287/150‑1 (MQ=255)
                                                                                                                         agagTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCCGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgat                        <  3:533896/150‑1 (MQ=255)
                                                                                                                           agTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                              <  4:805448/142‑1 (MQ=255)
                                                                                                                           agTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                              >  3:805448/1‑142 (MQ=255)
                                                                                                                             tATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtc                    <  1:1359832/150‑1 (MQ=255)
                                                                                                                               ttGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                  <  4:1024878/150‑1 (MQ=255)
                                                                                                                                tGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttc                 >  4:667775/1‑150 (MQ=255)
                                                                                                                                 gcaaaTGGCAAGAGACGCAACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATACGCGCGCGGCATCCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct                <  4:618875/147‑1 (MQ=255)
                                                                                                                                 gCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct                >  2:2365934/1‑150 (MQ=255)
                                                                                                                                  cTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTg               >  3:1326812/1‑150 (MQ=255)
                                                                                                                                    aaTGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt             <  2:373752/150‑1 (MQ=255)
                                                                                                                                       ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa          <  3:863684/150‑1 (MQ=255)
                                                                                                                                       ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa          >  4:765166/1‑150 (MQ=255)
                                                                                                                                       ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa          <  1:2339467/150‑1 (MQ=255)
                                                                                                                                       ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa          <  3:213208/150‑1 (MQ=255)
                                                                                                                                        gCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATATCGCGCggg                                                                         <  1:1064529/86‑2 (MQ=255)
                                                                                                                                        gCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCt                            >  2:1986501/1‑131 (MQ=255)
                                                                                                                                        gCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGAc         >  3:938008/1‑150 (MQ=255)
                                                                                                                                         cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCgg                                              <  3:310060/112‑1 (MQ=255)
                                                                                                                                          aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTTGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTg       <  4:1326812/150‑1 (MQ=255)
                                                                                                                                          aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgcg                                                                 <  2:276029/92‑1 (MQ=255)
                                                                                                                                          aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGTCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTg       >  2:1733250/1‑150 (MQ=255)
                                                                                                                                           agagACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggccgg                                                   <  3:1106239/105‑1 (MQ=255)
                                                                                                                                           agagACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTgg      >  4:394454/1‑150 (MQ=255)
                                                                                                                                            gagaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGcc                             <  1:746343/126‑1 (MQ=255)
                                                                                                                                               aCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGa  >  4:1545349/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGA  >  NC_000913_3_bme_pgi/4233625‑4233917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: