Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 3,815,761 G→T P3Q (CCA→CAA)  pyrE ← orotate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,815,7610GT97.6% 758.0 / 4.5 209P3Q (CCA→CAA) pyrEorotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (97/107);  total (99/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAT  >  NC_000913_3_bme_pgi/3815615‑3815904
                                                                                                                                                  |                                                                                                                                               
aaCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGTTATTGtt                                                                                                                                              <  4:1303505/150‑1 (MQ=255)
aaCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGtt                                                                                                                                              >  1:1917486/1‑150 (MQ=255)
  cGGCCTAACAGTGCTAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTc                                                                                                                                            <  2:1364124/150‑1 (MQ=255)
  cGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTc                                                                                                                                            <  2:2147339/150‑1 (MQ=255)
  cGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTc                                                                                                                                            <  2:878345/150‑1 (MQ=255)
   ggCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCa                                                                                                                                           <  1:1618688/150‑1 (MQ=255)
    gCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCAt                                                                                                                                          <  1:214836/150‑1 (MQ=255)
        aaCAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCt                                                                                                                                      >  2:1061176/1‑150 (MQ=255)
         aCAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCtt                                                                                                                                     <  3:1026236/150‑1 (MQ=255)
                cAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcct                                                                                                                              >  2:2268768/1‑150 (MQ=255)
                        gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTa                                                                                                                       >  3:997228/1‑149 (MQ=255)
                        gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTAc                                                                                                                      <  2:1710103/150‑1 (MQ=255)
                           cGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACtt                                                                                                                    <  4:558985/149‑1 (MQ=255)
                            ggTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACtttt                                                                                                                  <  1:784856/150‑1 (MQ=255)
                              tATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                                                <  1:1796580/150‑1 (MQ=255)
                                ttAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTAc                                                                                                              >  4:979280/1‑150 (MQ=255)
                                  aaaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAg                                                                                                            <  3:1370385/150‑1 (MQ=255)
                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGa                                                                                                           >  1:821285/1‑150 (MQ=255)
                                    aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACtt                                                                                                                    >  1:548747/1‑140 (MQ=255)
                                        cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaa                                                                                                      >  1:792619/1‑150 (MQ=255)
                                        cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaa                                                                                                      >  1:853795/1‑150 (MQ=255)
                                         ccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACtttt                                                                                                                  >  1:604322/1‑137 (MQ=255)
                                         ccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACtttt                                                                                                                  <  2:604322/137‑1 (MQ=255)
                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGc                                                                                                                                        <  1:475214/113‑1 (MQ=255)
                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGc                                                                                                                                        >  2:475214/1‑113 (MQ=255)
                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaa                                                                                                      <  1:351751/147‑1 (MQ=255)
                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaa                                                                                                      >  2:351751/1‑147 (MQ=255)
                                              gTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAgg                                                                                                <  1:2127763/150‑1 (MQ=255)
                                                       aTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtcatc                                                                                       >  4:927671/1‑150 (MQ=255)
                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtca                                                                                         <  3:855571/144‑1 (MQ=255)
                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                                                   >  3:252240/1‑150 (MQ=255)
                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                                                   >  3:1440086/1‑150 (MQ=255)
                                                             gCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGc                                                                                 <  2:853795/150‑1 (MQ=255)
                                                              cTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGcc                                                                                <  3:1223883/150‑1 (MQ=255)
                                                                  tGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACa                                                                                                             <  1:1449028/117‑1 (MQ=255)
                                                                  tGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACa                                                                                                             >  2:1449028/1‑117 (MQ=255)
                                                                  tGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaa                                                                            >  1:393908/1‑150 (MQ=255)
                                                                   gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCttttt                                                                           <  2:1917486/150‑4 (MQ=255)
                                                                     gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACt                                                                                                                     >  2:2427021/1‑106 (MQ=255)
                                                                     gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTa                                                                                                               >  3:821172/1‑112 (MQ=255)
                                                                     gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTa                                                                                                               <  4:821172/112‑1 (MQ=255)
                                                                     gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaaa                                                                         >  2:2130073/1‑150 (MQ=255)
                                                                       ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTc                                                                                                                 <  2:2149689/108‑1 (MQ=255)
                                                                       ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACa                                                                                                             <  2:1830679/112‑1 (MQ=255)
                                                                         ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAg                                                                     >  2:2369962/1‑150 (MQ=255)
                                                                            tttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                  <  1:2268768/150‑1 (MQ=255)
                                                                            ttgcAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTTGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTAAAGTCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                  <  2:763669/147‑1 (MQ=255)
                                                                             ttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTg                                                                 <  4:678073/150‑1 (MQ=255)
                                                                              tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                <  3:979280/150‑1 (MQ=255)
                                                                                   gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                           >  1:726332/1‑150 (MQ=255)
                                                                                      aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccg                                                        >  2:968994/1‑150 (MQ=255)
                                                                                       aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc                                                       <  3:795221/150‑1 (MQ=255)
                                                                                         tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCtttttttt                                                                        >  4:855417/1‑125 (MQ=255)
                                                                                         tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCt                                                     >  1:946387/1‑150 (MQ=255)
                                                                                         tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCt                                                     >  1:1693990/1‑150 (MQ=255)
                                                                                          cGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt                                                    <  2:1867439/150‑1 (MQ=255)
                                                                                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                   <  2:831901/150‑1 (MQ=255)
                                                                                            ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                                                >  1:906441/1‑88 (MQ=255)
                                                                                            ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                                                <  2:906441/88‑1 (MQ=255)
                                                                                            ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGcc                                                                                <  3:352777/120‑1 (MQ=255)
                                                                                            ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                  >  3:694803/1‑150 (MQ=255)
                                                                                             cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                 >  1:2106929/1‑150 (MQ=255)
                                                                                             cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                 >  3:177581/1‑150 (MQ=255)
                                                                                              aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtc                                                                                          >  2:2076408/1‑108 (MQ=255)
                                                                                                   ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                           >  2:154128/1‑150 (MQ=255)
                                                                                                   ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                           >  1:1282317/1‑150 (MQ=255)
                                                                                                    tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                          <  3:927671/150‑1 (MQ=255)
                                                                                                    tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                          <  2:1760906/150‑1 (MQ=255)
                                                                                                     aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                         <  4:1460398/150‑1 (MQ=255)
                                                                                                      acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                        >  1:2135171/1‑150 (MQ=255)
                                                                                                        acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                               <  1:2471941/141‑1 (MQ=255)
                                                                                                        acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                               >  2:2471941/1‑141 (MQ=255)
                                                                                                           tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATgg                                   >  1:2169773/1‑150 (MQ=255)
                                                                                                            gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTc                                                                                                                 >  4:291241/1‑71 (MQ=255)
                                                                                                            gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                  <  2:393908/150‑1 (MQ=255)
                                                                                                            gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                  <  2:1282317/150‑1 (MQ=255)
                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                              >  1:1003577/1‑150 (MQ=255)
                                                                                                                 ctAAGCGCAAATTCAACAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                             <  1:2035662/150‑1 (MQ=255)
                                                                                                                    aGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                          <  4:753721/150‑1 (MQ=255)
                                                                                                                     gcgcAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTcc                         >  4:440318/1‑150 (MQ=255)
                                                                                                                     gcgcAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTcc                         <  2:1680798/150‑1 (MQ=255)
                                                                                                                      cgcAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccc                        <  1:2386719/150‑1 (MQ=255)
                                                                                                                      cgcAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccc                        <  2:792143/150‑1 (MQ=255)
                                                                                                                           aTTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                   <  3:440318/150‑1 (MQ=255)
                                                                                                                           aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                   <  1:2369962/150‑1 (MQ=255)
                                                                                                                            ttCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                  <  1:1061176/150‑1 (MQ=255)
                                                                                                                               aaTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                              <  3:558516/119‑1 (MQ=255)
                                                                                                                               aaTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa               >  2:952567/1‑150 (MQ=255)
                                                                                                                                aTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTGCAATGGATTCGATTCCCCTCGGGCCag              <  1:1833936/150‑1 (MQ=255)
                                                                                                                                aTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag              >  4:1446075/1‑150 (MQ=255)
                                                                                                                                aTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag              >  4:548405/1‑150 (MQ=255)
                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga             <  3:548405/150‑1 (MQ=255)
                                                                                                                                  aaaCTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGc           >  1:1715442/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGcc          <  1:968994/150‑1 (MQ=255)
                                                                                                                                       ggCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaaa                                                                                                     <  4:1143910/56‑1 (MQ=255)
                                                                                                                                        gcgcTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                          >  3:148704/1‑114 (MQ=255)
                                                                                                                                          gcTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                            <  4:231915/110‑1 (MQ=255)
                                                                                                                                          gcTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                            >  3:231915/1‑110 (MQ=255)
                                                                                                                                            tGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAt  <  3:141438/150‑1 (MQ=255)
                                                                                                                                             gATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGcc                >  1:1934940/1‑135 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAT  >  NC_000913_3_bme_pgi/3815615‑3815904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: