BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,815,761	G→T	P3Q (CCA→CAA)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,815,761	0	G	T	100.0%	422.9 / NA	114	P3Q (CCA→CAA)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (51/63); total (51/63)

Only 100 of 114 total aligned reads displayed.

AACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGC  >  NC_000913_3_bme_pgi/3815615‑3815895

aaCGGCCTAACAGTGCCGGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGtt                                                                                                                                     >  1:617911/1‑150 (MQ=255)

 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGttt                                                                                                                                    <  4:522477/150‑1 (MQ=255)

   ggCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATTGTTTCa                                                                                                                                  <  1:1301899/150‑1 (MQ=255)

    gCCTAACAGTGCCAGATCGCGCCTGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCAt                                                                                                                                 >  1:1233283/1‑150 (MQ=255)

    gCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCAt                                                                                                                                 <  4:106457/150‑1 (MQ=255)

      cTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTGAATAAACTGGCGCTGATATTGTTTCATGc                                                                                                                               >  2:838083/1‑150 (MQ=255)

       tAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGcc                                                                                                                              >  3:372995/1‑150 (MQ=255)

            gTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGc                                                                                                                         <  3:512786/150‑1 (MQ=255)

              gCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctc                                                                                                                       <  1:152194/150‑1 (MQ=255)

               ccAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcc                                                                                                                      >  3:292311/1‑150 (MQ=255)

               ccAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcc                                                                                                                      >  2:900293/1‑150 (MQ=255)

                cAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcct                                                                                                                     >  2:236524/1‑150 (MQ=255)

                 aGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcctc                                                                                                                    >  4:794485/1‑150 (MQ=255)

                  gATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCa                                                                                                                   >  2:1199592/1‑150 (MQ=255)

                    tCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTc                                                                                                                                   >  4:674557/1‑132 (MQ=255)

                    tCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTc                                                                                                                                   <  3:674557/132‑1 (MQ=255)

                    tCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATc                                                                                                                 <  3:448173/150‑1 (MQ=255)

                      gcgcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAACTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCtt                                                                                                               <  1:1040001/150‑1 (MQ=255)

                       cgcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTa                                                                                                              <  2:617911/150‑1 (MQ=255)

                        gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTAc                                                                                                             >  1:68279/1‑150 (MQ=255)

                         cccGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCGGATATTGTTTCATGCCTTCGctc                                                                                                                       >  1:517914/1‑139 (MQ=255)

                           cGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACttt                                                                                                          >  1:184981/1‑150 (MQ=255)

                              tATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                                       <  2:1233283/150‑1 (MQ=255)

                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCAt                                                                                                                  >  1:25554/1‑134 (MQ=255)

                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCAt                                                                                                                  <  2:25554/134‑1 (MQ=255)

                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACa                                                                                                    >  4:212345/1‑148 (MQ=255)

                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACa                                                                                                    <  3:212345/148‑1 (MQ=255)

                                   aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGa                                                                                                  <  4:479157/150‑1 (MQ=255)

                                      aGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaa                                                                                               <  2:443508/150‑1 (MQ=255)

                                       gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGtt                                                                                                                                     <  4:327159/111‑1 (MQ=255)

                                       gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGtt                                                                                                                                     >  3:327159/1‑111 (MQ=255)

                                       gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaa                                                                                              <  1:838083/150‑1 (MQ=255)

                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGa                                                                                                  >  2:191760/1‑142 (MQ=255)

                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGa                                                                                                  <  1:191760/142‑1 (MQ=255)

                                           ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaaaaa                                                                                          >  1:492192/1‑150 (MQ=255)

                                            gCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACaaaaaaaa                                                                                         >  3:521953/1‑150 (MQ=255)

                                                 gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      >  2:456414/1‑148 (MQ=255)

                                                 gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      <  1:456414/148‑1 (MQ=255)

                                                    gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtc                                                                                 <  1:121269/150‑1 (MQ=255)

                                                    gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtc                                                                                 >  3:11672/1‑150 (MQ=255)

                                                        tAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtcatca                                                                             <  2:68279/150‑1 (MQ=255)

                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      <  1:994475/138‑1 (MQ=255)

                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      >  2:994475/1‑138 (MQ=255)

                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                                          <  3:512653/150‑1 (MQ=255)

                                                           gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                                          <  2:492192/150‑1 (MQ=255)

                                                             gCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctc                                                                                                                       <  1:1013790/103‑1 (MQ=255)

                                                             gCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctc                                                                                                                       >  2:1013790/1‑103 (MQ=255)

                                                             gCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGc                                                                        >  2:1285990/1‑150 (MQ=255)

                                                               ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCt                                                                      >  4:127361/1‑150 (MQ=255)

                                                                tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCtt                                                                     <  3:414442/150‑1 (MQ=255)

                                                                   gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctc                                                                                                                       <  1:891029/97‑1 (MQ=255)

                                                                   gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctc                                                                                                                       >  2:891029/1‑97 (MQ=255)

                                                                      gccGGATTGAAGCGTAAACTCGCGAAACTTTAACACCTGCTTGCTGAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAAACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                               <  4:168879/149‑1 (MQ=255)

                                                                       ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTATGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                                              >  4:445620/1‑150 (MQ=255)

                                                                        cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                                             <  1:1261281/150‑1 (MQ=255)

                                                                        cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                                             <  1:229220/150‑1 (MQ=255)

                                                                          gATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGt                                                           <  3:445620/150‑1 (MQ=255)

                                                                             ttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTg                                                        <  1:969242/150‑1 (MQ=255)

                                                                              tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCa                                                                                                                                  <  1:26247/75‑1 (MQ=255)

                                                                              tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCa                                                                                                                                  >  2:26247/1‑75 (MQ=255)

                                                                               cAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCt                                                                      <  1:143571/134‑1 (MQ=255)

                                                                               cAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCt                                                                      >  2:143571/1‑134 (MQ=255)

                                                                                   gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                  >  4:411342/1‑150 (MQ=255)

                                                                                       aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATAAGAAGACTTAAAAATCAGTTTGCCAGcgccgc                                              <  4:292311/150‑1 (MQ=255)

                                                                                        cTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCgccgcc                                             >  2:461639/1‑150 (MQ=255)

                                                                                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                          <  3:127361/150‑1 (MQ=255)

                                                                                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                          <  4:691136/150‑1 (MQ=255)

                                                                                              aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                       <  1:948114/150‑1 (MQ=255)

                                                                                              aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                       >  1:109015/1‑150 (MQ=255)

                                                                                                aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                     <  1:1199592/150‑1 (MQ=255)

                                                                                                    tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                 <  2:594320/150‑1 (MQ=255)

                                                                                                      acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGctcct                                                                                                                     <  4:447659/64‑1 (MQ=255)

                                                                                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGttt                                                                                                                                    <  4:342757/46‑1 (MQ=255)

                                                                                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGttt                                                                                                                                    >  3:342757/1‑46 (MQ=255)

                                                                                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                               <  1:445236/115‑1 (MQ=255)

                                                                                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                               >  2:445236/1‑115 (MQ=255)

                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      >  1:1260258/1‑85 (MQ=255)

                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc                                                                                      <  2:1260258/85‑1 (MQ=255)

                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaa                                                                 <  4:757715/106‑1 (MQ=255)

                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaa                                                                 >  3:757715/1‑106 (MQ=255)

                                                                                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                      >  1:423667/1‑149 (MQ=255)

                                                                                                                 ctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                  <  2:508687/120‑1 (MQ=255)

                                                                                                                 ctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                    <  4:372995/150‑1 (MQ=255)

                                                                                                                 ctAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                    <  1:461639/150‑1 (MQ=255)

                                                                                                                  tAAGCGCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat                   >  2:620688/1‑150 (MQ=255)

                                                                                                                         aaaTTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc            >  2:43754/1‑150 (MQ=255)

                                                                                                                           aTTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTATATAGAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg          <  4:613149/150‑1 (MQ=255)

                                                                                                                             tCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc        <  4:149524/150‑1 (MQ=255)

                                                                                                                               aaTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                                             <  3:591510/95‑1 (MQ=255)

                                                                                                                               aaTAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                                             >  4:591510/1‑95 (MQ=255)

                                                                                                                               aaTAAACTGGCGCTGATATTGTTTCATGACTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa      >  3:44284/1‑150 (MQ=255)

                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAg                                                            <  1:898141/94‑1 (MQ=255)

                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAg                                                            >  2:898141/1‑94 (MQ=255)

                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga    <  1:1285990/150‑1 (MQ=255)

                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga    <  2:184981/150‑1 (MQ=255)

                                                                                                                                 tAAACTGGCGCTGATATTGTTTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGAC‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCACTCGGGCCagag   >  1:580436/1‑150 (MQ=255)

                                                                                                                                   aaCTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGc  <  3:411342/150‑1 (MQ=255)

                                                                                                                                              atatTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa      >  4:403847/1‑135 (MQ=255)

                                                                                                                                              atatTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa      <  3:403847/135‑1 (MQ=255)

                                                                                                                                               tatTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                        >  3:220135/1‑116 (MQ=255)

AACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGC  >  NC_000913_3_bme_pgi/3815615‑3815895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`