Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,235,408 Δ1 bp intergenic (‑2175/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,235,4070A.97.9% 408.0 / ‑1.0 93intergenic (‑2174/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (37/54);  total (38/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG  >  NC_000913_3_bme_pgi/4235259‑4235551
                                                                                                                                                    |                                                                                                                                                
tCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                  <  2:377647/149‑1 (MQ=255)
tCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                  <  2:930048/149‑1 (MQ=255)
tCAAGGCCTTCGAACAATGGGGAGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGATTGTATCGGGCAAGGAAGGGGCTATTGTTCGCGACGCTTCCACGCGAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                                  <  2:381110/149‑1 (MQ=255)
  aaTGCCGTCGATCAACGGGGCGTGGAACTGGGTAAGGAACCGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                                <  1:980064/149‑3 (MQ=255)
   aTGCCTTCGATCAATGGGTCGAGGAACTGAGTAAGCAACTGGCGACGGAACTTTCGCCGGTTCTATCGGGCAAGGAAGGCGCCAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatca                                                                                                                                               <  2:533622/149‑4 (MQ=255)
     gCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatcatc                                                                                                                                            <  1:1071617/149‑1 (MQ=255)
       cttgggtCAATGGGGGGTGGAACTGGGTAAGGGAGTGGCGGCGGAAGTTGTGGCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgt                                                                                                                                          <  2:517104/143‑1 (MQ=255)
       ctgcggtCAATGGGGCGGGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgt                                                                                                                                          <  2:851220/143‑1 (MQ=255)
       cTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTGTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTAATCAtcgt                                                                                                                                          >  1:585774/1‑149 (MQ=255)
       cTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGTCGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgt                                                                                                                                          >  2:1023694/1‑149 (MQ=255)
       cTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgt                                                                                                                                          <  1:365592/149‑1 (MQ=255)
       cTTCGATCAAGGGGGCGTGGAACTGGGTAAGGAACTGGCGACGCAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATTCGCGAAGGAAAGCTTGATCAtcgt                                                                                                                                          <  1:591694/149‑1 (MQ=255)
         tCGATCAATGGCGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGGTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtcg                                                                                                                                        >  2:761115/1‑149 (MQ=255)
          cGATTAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGa                                                                                                                                       <  1:1060152/149‑1 (MQ=255)
          cGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAATCTTGATCATCGTCGa                                                                                                                                       <  1:838716/149‑1 (MQ=255)
            aTCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGata                                                                                                                                     >  1:778382/1‑149 (MQ=255)
                aTGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTa                                                                                                                                 <  1:508294/149‑1 (MQ=255)
                     gCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                                                                            >  1:423215/1‑149 (MQ=255)
                     gCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                                                                            <  2:1001518/149‑1 (MQ=255)
                     gCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                                                                            >  1:140792/1‑149 (MQ=255)
                       gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCCTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGa                                                                                                                          >  2:60643/1‑149 (MQ=255)
                       gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGa                                                                                                                          >  1:176631/1‑149 (MQ=255)
                        tGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGCGTGGTCGCGACGCTTCCACGCAAGGGTTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATAGTCGATATGTTGGTCGGAt                                                                                                                         >  1:897593/1‑149 (MQ=255)
                         ggAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATa                                                                                                                        <  1:732860/149‑1 (MQ=255)
                           aaCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                      <  2:641071/149‑1 (MQ=255)
                            accgggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                     <  1:5751/146‑1 (MQ=255)
                               gggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                  >  1:412721/1‑149 (MQ=255)
                                ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCTAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                 <  1:442245/149‑1 (MQ=255)
                                ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                                >  2:969424/1‑117 (MQ=255)
                                ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                                <  1:969424/119‑3 (MQ=255)
                                ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                 <  2:140792/149‑1 (MQ=255)
                                ggTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                 <  1:645320/149‑1 (MQ=255)
                                    aGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTAGCGACGATTCCACGGAAGGGCTTGTGACGCATCTGAATGCGCGAGGGGAAGCTTGATCGTCGTCGAGATGTAGGCCGCATAAGGCGttgagc                                                                                                             >  1:1091075/1‑145 (MQ=255)
                                          tGGCGACGGAACTTTTGCCGGTTGTATCGGGGAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                       <  2:355137/149‑1 (MQ=255)
                                             cgccGGAACTTTTGCCGGGTGTATCGGGCAAGGAAGGGGCCGGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                    <  2:895659/146‑1 (MQ=39)
                                             cGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                    <  2:863468/149‑1 (MQ=255)
                                              gACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                   >  1:66223/1‑149 (MQ=255)
                                              gACGGAACTTTTGCCGGATGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCGACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                   >  1:446322/1‑149 (MQ=255)
                                                 ggAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                <  1:833884/149‑1 (MQ=255)
                                                  gAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                               <  1:718142/149‑1 (MQ=255)
                                                  gAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                               >  2:426107/1‑149 (MQ=255)
                                                     cTTTTGCGGTGTGTAGCGGGCAAGGAAGGGGCGAGTGGGCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCGGCATGCGTGAAGTAGAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                            <  2:182000/149‑1 (MQ=37)
                                                      ttttGCCGGTTGTATCGGGCAAGGTAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGTAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                           <  1:872069/149‑1 (MQ=255)
                                                      ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCTCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                           >  1:774155/1‑149 (MQ=255)
                                                      ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGCAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                           >  1:1108104/1‑149 (MQ=255)
                                                      ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                           >  1:494456/1‑149 (MQ=255)
                                                      ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACACCGCATCCGGCAACCGAt                                                                                           >  2:320337/1‑149 (MQ=255)
                                                                   aTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAGGGAAAGCTTGATCATCGTCGATATGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCACCCGATGCCTGATGCGAcg                                                                              >  1:306769/1‑149 (MQ=255)
                                                                   aTCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                              >  1:641115/1‑149 (MQ=255)
                                                                          aagtaaggGTCGAGTGGCCGCGACGCTTCGACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                       <  1:232/149‑1 (MQ=38)
                                                                          aaggaaggGGCGCGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGCCAGCTTGATCATCGTGGATAGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGAcgcgaccgc                                                                       <  1:821859/149‑6 (MQ=37)
                                                                            ggaaggGGGGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                     <  1:142483/149‑1 (MQ=255)
                                                                              aaggGGCGAGTGGTCGCGGCGCTTCCACGCAAGCGCTTGTCGCGCATCGGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                   <  1:900411/149‑1 (MQ=255)
                                                                               aggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACTGATGCCTGGTGCGACGCGGTCGCGTCtt                                                                  >  1:231900/1‑149 (MQ=255)
                                                                                ggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                 >  1:555912/1‑149 (MQ=255)
                                                                                  ggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                               <  2:555912/149‑1 (MQ=255)
                                                                                   gCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                              <  2:327467/149‑1 (MQ=255)
                                                                                   gCGAGTGGTCGCGACGCCTCCACGCAAGGGCTTCTCGTTAATTTTCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                              <  1:590456/149‑1 (MQ=25)
                                                                                      aGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                           <  1:360416/149‑1 (MQ=255)
                                                                                      aGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                           <  1:886731/149‑1 (MQ=255)
                                                                                      aGTGGACGCGTCGCTGCCACGAAAGGGTGTGTCGAGCATATGATTGGCTGAAGGAAAGCTTGATCATAGTCGATATGTAGGCCTGATAAGGCTTTCACGCCGCAGCCGGCAACCGATGCCTGCTGCGACGCGGTCGCGTCTTATCAGGc                                                           <  2:1078384/149‑1 (MQ=255)
                                                                                       gTGGTCGCGAGGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGATAGAACAGCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                          <  2:405762/149‑1 (MQ=37)
                                                                                         ggTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                        <  2:289867/149‑1 (MQ=255)
                                                                                             gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                    >  1:441288/1‑149 (MQ=255)
                                                                                              cgACGTGTCCAGGCTAGGGCTTGTCGCGCATCGGCATGCGCGAAGGAAAGCTTGATCATCGTCGAGATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACCCGGTCGCGTCTTATCAGGCCTACAGGt                                                   <  2:1091075/149‑1 (MQ=25)
                                                                                              cgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                   <  1:426107/149‑1 (MQ=255)
                                                                                               gACGGTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAAGCCGAATCTGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                  <  1:320337/149‑1 (MQ=37)
                                                                                               gACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGAGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                  >  1:670487/1‑149 (MQ=255)
                                                                                                aCGCTTCCACGGAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                 <  1:191497/149‑1 (MQ=255)
                                                                                                aCGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                 >  1:439034/1‑149 (MQ=255)
                                                                                                    ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                             >  1:963846/1‑149 (MQ=255)
                                                                                                    ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                             >  2:811327/1‑149 (MQ=255)
                                                                                                    ttCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                             >  1:31440/1‑149 (MQ=255)
                                                                                                    ttCCACGCAAGGGCTTGGCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                             <  1:88323/149‑1 (MQ=255)
                                                                                                    ctCCACGCAAGGGCTGGGCGCGCATCTGCATGGGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                             <  2:453003/148‑1 (MQ=25)
                                                                                                     tcgaCGTAAGGGCTTGTCGCGCATCTGCACGCGCGAAGGAAAGCGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                            <  2:312351/146‑1 (MQ=25)
                                                                                                        aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGACGATATGTAGGCCGCAAAAAGCGTTCCCACCGCCACCGACAACCGATACCTGCCACGACGCGGCCGCGTCTCATCAGGCCTACAGGTCGATGCCGat                                         >  2:380018/1‑149 (MQ=255)
                                                                                                         cGCAAGGGCTTGTCGGGCGTCGGCATGCGCGAAGGAAAGCTTGATCAGCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATTCGACGCGGTCGCGTCTTGTCAGGCCTACAGGTCGATGCCGata                                        <  1:846226/149‑1 (MQ=37)
                                                                                                         cGCAAGGGCCTGTCGCGCATCCGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAACAGGCCTACAGGTCGATGCCGata                                        <  2:717943/149‑1 (MQ=38)
                                                                                                                 cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                                <  2:176631/149‑1 (MQ=255)
                                                                                                                 cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                                <  2:66223/149‑1 (MQ=255)
                                                                                                                  ttGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCCGGCCTACAGGTCGATGCCGATATGTACATCg                               >  1:479536/1‑149 (MQ=255)
                                                                                                                    gTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                             >  2:445626/1‑117 (MQ=255)
                                                                                                                    gTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                             <  1:445626/117‑1 (MQ=255)
                                                                                                                      cgcgTATCGGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                           <  2:480726/149‑1 (MQ=255)
                                                                                                                       gcgcATCTGCATGGGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAGGCCGATATGTACATCGTATTc                          <  2:979096/149‑1 (MQ=255)
                                                                                                                       gcgcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                          <  2:963846/149‑1 (MQ=255)
                                                                                                                        ggtaTCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGAGATGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCAACCGATGCTTGATTCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                         <  1:100658/146‑1 (MQ=37)
                                                                                                                         gcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                        >  1:626244/1‑149 (MQ=255)
                                                                                                                          cATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                       <  2:412721/149‑1 (MQ=255)
                                                                                                                          cATCGGCAGGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                       <  1:630811/149‑1 (MQ=255)
                                                                                                                            tCTGCGTGCTCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                     <  1:760172/149‑1 (MQ=255)
                                                                                                                              tGCATGCGGGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                   <  2:977451/149‑1 (MQ=255)
                                                                                                                                 aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                     >  2:263175/1‑96 (MQ=255)
                                                                                                                                 aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                     <  1:263175/96‑1 (MQ=255)
                                                                                                                                 aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                >  2:1063719/1‑149 (MQ=255)
                                                                                                                                     gcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCGACAGGTCGATGCCGATATGTACATCGTATTCGCCAATTAATACAt            >  2:793816/1‑149 (MQ=255)
                                                                                                                                           gAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACg      >  1:557920/1‑149 (MQ=255)
                                                                                                                                              aGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt   >  2:634315/1‑149 (MQ=255)
                                                                                                                                               gCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg  >  2:540534/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG  >  NC_000913_3_bme_pgi/4235259‑4235551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: