New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 1686709 =40 (0.870)4 (0.090) 4/272 6.3 9.0% intergenic (‑121/+22) fumC/fumA fumarate hydratase (fumarase C),aerobic Class II/fumarate hydratase (fumarase A), aerobic Class I
?NC_000913_3_bme_pgi 1686725 = 43 (0.980)intergenic (‑137/+6) fumC/fumA fumarate hydratase (fumarase C),aerobic Class II/fumarate hydratase (fumarase A), aerobic Class I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGGAACTGGGAATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/1686861‑1686709
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcggCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAA  >  NC_000913_3_bme_pgi/1686725‑1686870
                                                                                                                                                                                                                                                                                                           
ctgcagTGGTAATGTAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGG                                                                                                                                                        <  1:349837/143‑1
  gcagTGGGTATGGAAGCCATCTGGAAAATTGGAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGGCCGTCGGGGC                                                                                                                                                      <  2:345847/145‑1
  GAACTGGGAATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGCAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCCGTGTGAAATAAACAGACCCGCACTTCGGGGC                                                                                                                                                      >  2:787489/1‑149
                            AATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGG                                                                                                                                                        <  1:181513/121‑1
                            AATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGG                                                                                                                                                        >  2:181513/1‑121
                                                                                                                 ACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTAT                                                                                >  2:232367/1‑108
                                                                                                                 ACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGGGCGGCTCCGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTAT                                                                                <  1:232367/108‑1
                                                                                                                                     AGAGCCGCCCTTCGGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAT                      >  1:762173/1‑146
                                                                                                                                     AGAGCCGCCCTTCGGGGCGGCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCAT                      <  2:762173/146‑1
                                                                                                                                                  aggcctGCTCTGTTCATTCCACACAGCGGGTGCATTGTGTGAGTTGGATCTGCTGGAAGAAGGCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATAT      <  2:12108/143‑1
                                                                                                                                                      CGGCTCTGTTTATTTCACACTGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAACAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCTATTCTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAA  <  2:615203/149‑1
                                                                                                                                                                                                                                                                                                           
CGGAACTGGGAATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACTTCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAAACAGAGCCGCCCTTCGGGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/1686861‑1686709
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcggCTCTGTTTATTTCACACAGCGGGTGCATTGTGTGAGTTGTATCTGCTGGAAGAAGTCATTTCCTTTATCATCCACAAGGATAAACGCCGGGAAATCTTCCACTTCAATTTTCCAGATGGCTTCCATTCCCAGTTCCGGATATTCAA  >  NC_000913_3_bme_pgi/1686725‑1686870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.