New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 314062942 (0.910)4 (0.090) 4/268 6.2 8.6% coding (171/489 nt) hybE hydrogenase 2‑specific chaperone
?NC_000913_3_bme_pgi = 3140635 45 (1.040)coding (165/489 nt) hybE hydrogenase 2‑specific chaperone
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_bme_pgi/3140480‑3140629
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acggggtgTGTGATCACCCCGTGGATGCTGAGTGCAGTTATCTTCCCCGGCCCGGATCAACTCTGGCCGCTGCGCAAAGTGAGTGAAAAAATTGGTCTGCAACTGCCGTATGGCACTATGACCTTTACCGTTGGCGAACTGGACGGTGTTTCGCA  <  NC_000913_3_bme_pgi/3140635‑3140489
                                                                                                                                                                                                                                                                                                         
GAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCACACCAATTTTTTCACTCACTTTGCGCAGCGGCCCGAGTTGATCCGGGCCGGGGAAGAACACTGCACTCAGCATCCACGGGGT                                                                                                                                                      >  1:196774/1‑149
             AAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGTGTGATCACCCC                                                                                                                                         >  1:316466/1‑149
               ACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTGTGTGATCACCCCGT                                                                                                                                       <  2:316466/149‑1
                                         GGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGG                                                                                                                                                        >  1:38206/1‑106
                                         GGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGG                                                                                                                                                        <  2:38206/106‑1
                                                                                                                          GATAACTGCACTCAGCATCCACGGGGTGTGTGATCACCCCGTGGATGCT                                                                                                                                <  1:780435/49‑1
                                                                                                                          GATAACTGCACTCAGCATCCACGGGGTGTGTGATCACCCCGTGGATGCT                                                                                                                                >  2:780435/1‑49
                                                                                                                                              ACGGGGTGTGTGATCACCCCGTGGATGCTGAGTGCAGTTATCTTCCCCGGCCCGGATCAACTCTGGCCGCTGCGCAAAGTGAGTGAAAAAATTGGTCTGCAACTGCCGTATGGCACTATGACCTTTACCGTTGGCGAACTGGACGGTGT        <  2:536723/149‑1
                                                                                                                                                    TGTGTGATCACCCCGTGGATGCTGAGTGCAGTTATCTTCGCCGGCCCGGATGAACTCTGGCCGCTGCGCAAAGTGAGTGACAACATTGGTCTACACCTGCCGTATGGCACTATGACCTTTACCGTTACCGTCCTAGACGGTATTTCGCA  >  2:533007/1‑149
                                                                                                                                                                                                                                                                                                         
GAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAGCATCCACGGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_bme_pgi/3140480‑3140629
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acggggtgTGTGATCACCCCGTGGATGCTGAGTGCAGTTATCTTCCCCGGCCCGGATCAACTCTGGCCGCTGCGCAAAGTGAGTGAAAAAATTGGTCTGCAACTGCCGTATGGCACTATGACCTTTACCGTTGGCGAACTGGACGGTGTTTCGCA  <  NC_000913_3_bme_pgi/3140635‑3140489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.