New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	3243907 =	53 (1.150)	5 (0.110)	5/284	6.0	8.4%	coding (835/1413 nt)	uxaC	uronate isomerase
	?	NC_000913_3_bme_pgi	3243926 =	56 (1.220)					coding (816/1413 nt)	uxaC	uronate isomerase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAG  >  NC_000913_3_bme_pgi/3243762‑3244050                                                                                                                                                   |                                                                                                                                                gCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGc                                                                                                                                              <  1:572942/149‑1 (MQ=255)  ccATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGTACCGGCGCGGAATGGCGTTGCGCAGCGAGCCAGTGTAGAACTGCGTGTA‑CATCCCGCGGGTGGCCTAATCAGCAACAAGGAAAACCAGTAccccc                                                                                                                                             >  2:128063/1‑146 (MQ=255)  ccATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCa                                                                                                                                             >  2:323983/1‑149 (MQ=255)   cATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TATACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAg                                                                                                                                            >  1:325926/1‑149 (MQ=255)    aTCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTAcccccgt                                                                                                                                           >  2:206909/1‑144 (MQ=255)          tgggcGTTGATTGGGTCAAAGCCTACATCCGGCCCCAGCAGTGTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACCGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGaa                                                                                                                                     <  2:206017/145‑1 (MQ=255)               gTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTgcg                                                                                                                                >  1:517625/1‑149 (MQ=255)               gTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTgcg                                                                                                                                >  2:742345/1‑149 (MQ=255)                ttGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTgcgc                                                                                                                               <  2:454989/149‑1 (MQ=255)                       aGTCAAAAGCTGCATCTGGCCCCAGCATTTTGAATTGACGCAGGTTGTTATTACGCAGTGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTGCCGCAGTGTTGTACTGCGCCACTTCg                                                                                                                        <  2:277677/149‑1 (MQ=255)                        gTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAAGGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCAcgtcgt                                                                                                                       <  2:555672/149‑1 (MQ=255)                               ccTACACCCGGCCCCGGCAGCTTGAACGGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTgctcgct                                                                                                                <  2:435568/149‑1 (MQ=255)                                 tACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATATGGTACTGC‑TGTACCCAGCCGCGACGTGCCTATTCGGCACCAAAGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCa                                                                                                              >  2:654337/1‑149 (MQ=255)                                   cATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAgg                                                                                                            <  1:551421/149‑1 (MQ=255)                                   cATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAgg                                                                                                            <  1:558543/149‑1 (MQ=255)                                         gCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGCACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCCGGGTTTCg                                                                                                      >  2:329120/1‑149 (MQ=255)                                            ccAGCCGTTCGAACCGACGCAGGTTGCTATAACGCAGCGCGCCAATGCGGTACTGC‑TGTACCCAGCCGCGACGTGCAGATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGAGCTCGCTCAGGGTTTCgcca                                                                                                   <  2:52285/149‑1 (MQ=255)                                            ccAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCgcca                                                                                                   <  1:73132/149‑1 (MQ=255)                                            ccAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCgcca                                                                                                   <  2:124212/149‑1 (MQ=255)                                                    ttGACCTGACGCAGGCCTGTATGACGCAGCGCGCCAAAGCGGTACTCC‑TGTACCCAGCCGCGACGTGCATATCCTGCACCAAGGAACACCAGTACCGCAGTTTTGACCCGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACg                                                                                           <  2:428848/149‑1 (MQ=255)                                                        aCTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGgcgc                                                                                       >  2:92523/1‑149 (MQ=255)                                                        aCTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGccgt                                                                                       >  2:806540/1‑148 (MQ=255)                                                            aCGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCa                                                                                   <  1:51166/149‑1 (MQ=255)                                                            aCGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCa                                                                                   >  2:170662/1‑149 (MQ=255)                                                               cacggtgttATTACGCAGCGCGCTCATGTGGCACTGC‑TGTACCCAGCCGCGACTTGCATATTCGGCACCAAGGAACAGCAGTACCGCATTGTTGAACCGCGGCACTTCGTGCTCGCCTAGGGTTTCGCCAGACAGCCGGCGAGCCAGaa                                                                                <  2:596085/144‑1 (MQ=255)                                                                aGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAAt                                                                               >  1:369374/1‑149 (MQ=255)                                                                    tgttATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTg                                                                           <  1:170662/149‑1 (MQ=255)                                                                        attaCGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCa                                                                       <  1:323983/149‑1 (MQ=255)                                                                             gCAGCGCGCCAATGTTGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTcc                                                                  <  2:464001/149‑1 (MQ=255)                                                                                          gtgGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCGGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCtt                                                     <  2:30417/149‑1 (MQ=255)                                                                                             gTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAg                                                  >  1:134091/1‑149 (MQ=255)                                                                                              tACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGc                                                 >  1:304264/1‑149 (MQ=255)                                                                                                 tgc‑tgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCCTCCTTCGCTCCAGCaac                                              >  2:633597/1‑148 (MQ=255)                                                                                                   c‑tgTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACa                                            >  1:683266/1‑149 (MQ=255)                                                                                                       tACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATca                                         >  1:325536/1‑149 (MQ=255)                                                                                                        aCCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATcac                                        <  1:489433/149‑1 (MQ=255)                                                                                                        aCCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATcac                                        >  2:513584/1‑149 (MQ=255)                                                                                                        aCCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGCCGCGCCAGAATGCTGTCCAGTTCCGCTTCCTTCGCTTCAGCAAACATcac                                        >  2:513571/1‑149 (MQ=255)                                                                                                         cccAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCGGCATCCGGCTCGCCCAGGGGTGCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCACTTCAGCAAACATcacc                                       >  1:795838/1‑149 (MQ=255)                                                                                                         cccAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATcacc                                       <  1:92523/149‑1 (MQ=255)                                                                                                                     gTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGt                                                                                                                       <  1:717322/57‑1 (MQ=255)                                                                                                                     gTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGt                                                                                                                       >  2:717322/1‑57 (MQ=255)                                                                                                                        cATATTCGGCACCAAGCAACACCAGTAACGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCCGCAAACATCACCACGTCCAGCGCgggg                        >  2:551998/1‑149 (MQ=255)                                                                                                                               ggCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCGGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCatcca                 >  1:345919/1‑145 (MQ=255)                                                                                                                                  aCCAAGGAACACCAGTACCGCAGTTTGAAACTGCGCCACTTCGTGCTCGCCCAGGGTTTCGCAAACCAGGCGGCGCCCCAGAATACTGTCCAGTTCCGCTCCGTTCGCTTCCGCAAACATCAACACAACCACCGCGTGGTCAGagacct              >  2:137400/1‑147 (MQ=255)                                                                                                                                    cAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTa            >  2:606000/1‑149 (MQ=255)                                                                                                                                     aaGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTAc           <  1:272182/149‑1 (MQ=255)                                                                                                                                         aaCACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTGGCTTCAGCCAACATCACCCCATCCAGCGCTTGGTCAGACACTTTACGGcc       >  1:221304/1‑149 (MQ=255)                                                                                                                                              cAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGaa                                                                                <  1:38425/71‑1 (MQ=255)                                                                                                                                              cAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGaa                                                                                <  1:40644/71‑1 (MQ=255)                                                                                                                                              cAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGaa                                                                                >  2:40644/1‑71 (MQ=255)                                                                                                                                              cAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGaa                                                                                >  2:38425/1‑71 (MQ=255)                                                                                                                                              cAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGGGAg  >  1:228588/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                GCCATCGGACGGTCGTTGATGGAGTCAAAGCCTACATCCGGCCCCAGCAGTTTGAACTGACGCAGGTTGTTATTACGCAGCGCGCCAATGTGGTACTGC‑TGTACCCAGCCGCGACGTGCATATTCGGCACCAAGGAACACCAGTACCGCAGTTTTGAACTGCGCCACTTCGTGCTCGCTCAGGGTTTCGCCAGCCAGACGGCGCGCCAGAATGCTGTCCAGTTCCGCTTCGTTCGCTTCAGCAAACATCACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAG  >  NC_000913_3_bme_pgi/3243762‑3244050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.