New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 4256360 =96 (2.860)7 (0.210) 6/272 3.4 6.8% coding (108/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
?NC_000913_3_bme_pgi 4256386 = 100 (3.060)coding (82/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCCGCGCATTTGCATTATTAACCAGAGGCTTTACATCGTTTATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGGGGCTGGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/4256509‑4256360
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggatCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAA  >  NC_000913_3_bme_pgi/4256386‑4256532
                                                                                                                                                                                                                                                                                                         
TGCCGCGCATTTGCATTATTAACCAGAGGCTTTACATCGTTTATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGGGGCTGGA                                                                                                                                                      <  2:340561/149‑1
                                   ATCGTTTATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGGGGCTG                                                                                                                                                        >  1:539504/1‑112
                                   ATCGTTTATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGGGGCTG                                                                                                                                                        <  2:539504/112‑1
                                                                                                                   TTCCGGCAGATCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAG                                                                                                    <  1:234575/84‑1
                                                                                                                   TTCCGGCAGATCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAG                                                                                                    >  2:234575/1‑84
                                                                                                                       GGCAGATCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGC                               >  2:327517/1‑149
                                                                                                                            ATCCTGCCCCGGAACTGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTG                                                <  1:124921/127‑1
                                                                                                                            ATCCTGCCCCGGAACTGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTG                                                >  2:124921/1‑127
                                                                                                                            ATCCTGCCCCGGAACTCGGGCTGGATCTGCCGGCATAGACTTGCTTTTTACCAGGATGCTTAATGGTACATTCCGTAATTTGTAGTAAATTCGTGGCCAGCCGGCCCTAAACGATGTAAAGCCTCTGGCTAATACTGCCAATGTCCGGC                          >  2:430616/1‑149
                                                                                                                                   CCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGAAGGAAAGCATCTGGTTAATAATGCCAATGCGCGGCACGGATA                   >  2:411862/1‑149
                                                                                                                                                   GATCTGCCGGAATAGACTTGCTTTTTACAAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGGCTCTGGTTAATAATGCACATGCGCGGCAAGGATAGCAGAAAGTAGTGGGA   >  2:329397/1‑149
                                                                                                                                                    ATCTGAAGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGCTATCAGAAAGTCATGGGAg  >  2:102930/1‑148
                                                                                                                                                                                                                                                                                                         
TGCCGCGCATTTGCATTATTAACCAGAGGCTTTACATCGTTTATGTCCGGCTGGCCACGAATTTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGGGGCTGGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/4256509‑4256360
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggatCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAA  >  NC_000913_3_bme_pgi/4256386‑4256532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.