New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 4162839	97 (2.890)	4 (0.130)	4/266	4.1	4.3%	coding (432/1101 nt)	trmA	tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
	?	NC_000913_3_bme_pgi	= 4162849	87 (2.720)					coding (422/1101 nt)	trmA	tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGTTCGGCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTAC  >  NC_000913_3_bme_pgi/4162693‑4162984                                                                                                                                                    |                                                                                                                                                  gctttCGGCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTgcgcgcg                                                                                                                                                 <  1:109840/146‑1 (MQ=255)    ttCGGCCGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAAGCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAg                                                                                                                                              <  2:597027/149‑1 (MQ=255)       ggCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGc                                                                                                                                           <  1:479445/149‑1 (MQ=255)        gCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCCCATT‑CCGATTCTGCGAGCGCAGTCCa                                                                                                                                          >  1:249660/1‑149 (MQ=255)        gCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCa                                                                                                                                          >  2:513894/1‑149 (MQ=255)        gCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCa                                                                                                                                          <  1:513894/149‑1 (MQ=255)          tGGGTAAAGCTGTTTTCTACCTTACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATc                                                                                                                                        <  2:347025/149‑1 (MQ=255)          tGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGACCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATc                                                                                                                                        <  1:223688/149‑1 (MQ=255)          ggggTAAAGCCGTTTTCTACCTGACCGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGAACCCGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATc                                                                                                                                        <  2:302179/148‑1 (MQ=255)            ggTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑GAGATTCTGCGCGCGCAGTGCATcgc                                                                                                                                      >  1:588307/1‑149 (MQ=255)            ggTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATcgc                                                                                                                                      >  2:446664/1‑149 (MQ=255)                aaGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATcgaacag                                                                                                                                  >  1:461852/1‑144 (MQ=255)                 aGCTGTTTTGTACCTGACGGTAGAGCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAgg                                                                                                                                 <  2:395698/149‑1 (MQ=255)                      ttttCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTc                                                                                                                            >  1:250260/1‑149 (MQ=255)                      ttttCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTCCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTc                                                                                                                            >  1:532327/1‑149 (MQ=255)                        ttCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATTTAATCCTGATCCAGCTCGATTTTGGTTTGCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCg                                                                                                                          <  1:176411/149‑1 (MQ=255)                             ccTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTcc                                                                                                                     >  1:209819/1‑149 (MQ=255)                               tGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTg                                                                                                                   >  1:374308/1‑149 (MQ=255)                                gACGGTAGATCATCTCTTTCCCTGCGACCGGCAGCCGTTCATCGCTGTAATCTTCATCCAGCTCGATTTTGGTTTTCGTTGCACGACTATTTAGTTGCACTTT‑CAGCTCCTGCGCGCGCAGTGCCTCGCGCAGGGACCCCGCTTCCTgg                                                                                                                  <  2:382668/149‑2 (MQ=255)                                      aGATCATCTCTTTACCTGCGACCGGCAGAAGTTAATTGATGTAATCCTGAACCAGCTCGATTTTGATTTTCTATGCCCGACCAATAAGATGCAAATT‑CCGCTTCTGCGCGCGCAGGGCATCGCGCAGGGCCTCCGCTCGCGGACGCCAc                                                                                                            >  2:373817/1‑149 (MQ=255)                                        atcatcTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATATTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CTGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtc                                                                                                          >  2:219254/1‑149 (MQ=255)                                         tcatcTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtca                                                                                                         >  2:556370/1‑149 (MQ=255)                                          catcTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcat                                                                                                        >  1:507948/1‑149 (MQ=255)                                           atcTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCACTGCATCGCGCAGCGCCTCCGCTTCCTCCCGCCACtccgc                                                                                                       >  2:594446/1‑146 (MQ=255)                                             ctctTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTcatcat                                                                                                     <  2:390042/149‑1 (MQ=255)                                              tctTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTGGATTTTGGTTTTCGGTGCCAGACGAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCAGCTTCCTGACGCCACTCatcatc                                                                                                    >  2:198533/1‑149 (MQ=255)                                              tctTTCCCTGCGACCGGCAGACGTGCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGAATCCCGCAGGGCCTCCGCTTCCTGACGCCACTCatcatc                                                                                                    <  2:402372/149‑1 (MQ=255)                                                    ccTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGctt                                                                                              >  2:499699/1‑149 (MQ=255)                                                     cTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttc                                                                                             <  2:479119/149‑1 (MQ=255)                                                     cTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGACTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttc                                                                                             <  2:250260/149‑1 (MQ=255)                                                          cccGGCATACGTTCATCGATGTAATCCTGATCCCGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATg                                                                                        <  1:557479/148‑1 (MQ=255)                                                          aCCGGCAGACGTTCATCGATGTAATCCTTATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATg                                                                                        <  2:82899/149‑1 (MQ=255)                                                             cgtaGACGGTCTTCGACGTAATCCTGAACCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCATATGCACATT‑CCGATACTGCGCGCGCAGTTCATCTCGCAGGGCCTCCGCTTCCTGATGTCGCTCATCATCCAGCTTCTTATGGta                                                                                     <  2:418257/146‑1 (MQ=255)                                                              gCATACGTTCATTGAGTTAATCCTGATCCAGCTCCATTTTGGTTTTCGTTGCCCTACCAATCAGATGCACATT‑CAGATTCTGCGCGCTCAGTGCATCGCGCAGGTCTTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGtat                                                                                    <  2:384766/149‑1 (MQ=255)                                                              gCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTcc                                                                                                                     <  2:111370/116‑1 (MQ=255)                                                              gCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTcc                                                                                                                     >  1:111370/1‑116 (MQ=255)                                                               cAGGCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGtata                                                                                   <  1:10846/149‑1 (MQ=255)                                                                aGACGTTCATCGATTGAATCCTGATCCAGGTCGATTTTGGTTTTCGTTGACCGACGAAGCAGATGAACATT‑CAGATTCGGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAg                                                                                  <  2:227645/149‑1 (MQ=255)                                                                aGACGTTCATCGATGTAATACTGATCCAGCTCGATTTTGATTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAg                                                                                  <  1:97252/149‑1 (MQ=255)                                                                  aCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCa                                                                                >  2:524792/1‑149 (MQ=255)                                                                    gTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCTTTGCTCGATCACTCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCGTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg                                                                              <  1:329751/149‑1 (MQ=255)                                                                    gTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCTCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg                                                                              <  1:329647/149‑1 (MQ=255)                                                                    gTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGCCCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTCCATCGCGCAGGCCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGTTATAGCCgg                                                                              >  2:270946/1‑149 (MQ=255)                                                                      tCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTTCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCACGGa                                                                            >  2:114197/1‑149 (MQ=255)                                                                        aTCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGaaa                                                                          >  1:267673/1‑149 (MQ=255)                                                                           gATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATTGTATGGCAGGGAAaaca                                                                       >  2:235899/1‑149 (MQ=255)                                                                            aTGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAaccac                                                                      <  2:209819/149‑1 (MQ=255)                                                                            aTGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAaccac                                                                      >  1:458849/1‑149 (MQ=255)                                                                            aTGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGAGCGCAGTGCATAGCGCAGGGACTCCGCTTACTGACGACACTCATCATCCAGCTTCTTATGGTATAGAAAGGAAaacaa                                                                      >  1:243815/1‑148 (MQ=255)                                                                              gTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGAAAAaccacag                                                                    >  1:370560/1‑147 (MQ=255)                                                                               tAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCCGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAACTTCTTATGGTATAGCAGGGAAAACACCGc                                                                   >  1:268519/1‑149 (MQ=255)                                                                                aaTCCTGATCTAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCTGGGAACCAACCGcg                                                                  >  1:582127/1‑148 (MQ=255)                                                                                  tCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGACCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGAAAACCACCGCCTg                                                                >  1:193506/1‑149 (MQ=255)                                                                                     tGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATgg                                                                                       >  2:80385/1‑123 (MQ=255)                                                                                     tGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATgg                                                                                       <  1:80385/123‑1 (MQ=255)                                                                                      gATCCAGCTCGATTTTGGTTTTCGTTGCCTGACCAATCATATG‑ACTTTACTGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTa                                                            <  1:584377/149‑1 (MQ=255)                                                                                      gATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCCACCCGACGCCCCACTGCAGCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTa                                                            <  1:589403/149‑1 (MQ=255)                                                                                          cAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCa                                                        >  2:4634/1‑149 (MQ=255)                                                                                            gCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCa                                                                                                                                               >  2:111589/1‑60 (MQ=255)                                                                                            gCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCa                                                                                                                                               <  1:111589/60‑1 (MQ=255)                                                                                              tCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTAATGGTATAGCAGAGAAACCACCGCCTGATTACTCAgaga                                                    >  1:193774/1‑146 (MQ=255)                                                                                               cGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTa                                                   <  2:435539/149‑1 (MQ=255)                                                                                                   tttGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGa                                               >  1:375328/1‑149 (MQ=255)                                                                                                    ttGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTATTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAg                                              >  1:411425/1‑149 (MQ=255)                                                                                                     tGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAgg                                             <  1:524792/149‑1 (MQ=255)                                                                                                       gTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTa                                           >  1:343529/1‑149 (MQ=255)                                                                                                        ttttCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaa                                          >  1:577724/1‑149 (MQ=255)                                                                                                        ttttCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACAACCACAAGATTACTCAGAGTAGTGCGGTaa                                          >  1:555969/1‑149 (MQ=255)                                                                                                         tttCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaat                                         <  1:446664/149‑1 (MQ=255)                                                                                                             gTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaag                                     >  1:170625/1‑148 (MQ=255)                                                                                                                   cGACCAATCAGATGCACATG‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaa                               <  1:289274/149‑1 (MQ=255)                                                                                                                      ccAATCAGATGCAAATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaacaa                            <  2:305323/149‑1 (MQ=255)                                                                                                                       cAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaacaac                           <  2:532327/149‑1 (MQ=255)                                                                                                                        aaTCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcatc                                                                                                       <  2:3343/72‑1 (MQ=255)                                                                                                                        aaTCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcatc                                                                                                       >  1:3343/1‑72 (MQ=255)                                                                                                                           cAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTtgt                       <  1:4634/149‑1 (MQ=255)                                                                                                                            aGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGTTAATCAAACTGGAACAACTtgtg                      >  1:293723/1‑149 (MQ=255)                                                                                                                                gCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcgc                  >  1:559559/1‑149 (MQ=255)                                                                                                                                 cacaTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaat                                     <  1:162011/129‑1 (MQ=255)                                                                                                                                 cacaTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaat                                     >  2:162011/1‑129 (MQ=255)                                                                                                                                  acaTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAg                >  1:159302/1‑149 (MQ=255)                                                                                                                                    aTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg                                                                              >  2:228968/1‑85 (MQ=255)                                                                                                                                    aTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg                                                                              <  1:228968/85‑1 (MQ=255)                                                                                                                                    aTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACtt                         >  1:230402/1‑138 (MQ=255)                                                                                                                                    aTT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACtt                         <  2:230402/138‑1 (MQ=255)                                                                                                                                      t‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCTACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGACGAAcc            <  2:374308/149‑2 (MQ=255)                                                                                                                                      t‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGacag            <  2:159302/149‑4 (MQ=255)                                                                                                                                          gATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTCTAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGa         >  1:140037/1‑149 (MQ=255)                                                                                                                                          gATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGa         <  2:442737/149‑1 (MQ=255)                                                                                                                                          gATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGa         >  2:419501/1‑149 (MQ=255)                                                                                                                                           aTTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGat        <  2:559559/149‑1 (MQ=255)                                                                                                                                             tCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGatta      <  1:219254/149‑1 (MQ=255)                                                                                                                                             tCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGatta      <  1:252314/149‑1 (MQ=255)                                                                                                                                                gcgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGAttatta   <  1:235899/149‑1 (MQ=255)                                                                                                                                                 cgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTAc  <  1:499699/149‑1 (MQ=255)                                                                                                                                                 cgcgcgcAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGAAGTAAGATAATCAATCTAGAACAACATGTGGCTCAGAAAGGCATAATTAc  >  1:219674/1‑149 (MQ=255)                                                                                                                                                 cccgcacAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCATCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTAc  <  1:177079/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GCGTTCGGCTGGGTAAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATT‑CAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTAC  >  NC_000913_3_bme_pgi/4162693‑4162984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.