Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,7920AC42.5% 22.8 / 34.8 40S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base C (10/7);  total (25/15)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAA  >  NC_000913_3_bme_pgi/526647‑526907
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gCTGGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACAGGCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                  <  2:193441/149‑1 (MQ=255)
 cTTGCGCAGTGCGGGCCGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAgcgc                                                                                                                 <  2:571446/149‑1 (MQ=255)
  ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAATAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                <  2:406095/149‑3 (MQ=255)
           gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                       <  1:93224/149‑1 (MQ=255)
               gCTGACGTTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                   <  2:422097/149‑1 (MQ=11)
               gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                   <  1:250421/149‑1 (MQ=255)
                 tGACGGTGGAGCAACTGGCCAGAAAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATTAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                 <  1:569677/149‑1 (MQ=11)
                  gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACTGGCGTGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                <  1:365417/149‑1 (MQ=255)
                  gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                <  1:323684/149‑1 (MQ=255)
                     ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTAACAGCGCAGACAGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCa                                                                                             >  1:262723/1‑149 (MQ=255)
                       tGGAGCAACTTGCCAGAGAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                           <  2:477779/149‑1 (MQ=255)
                          aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                        <  2:567251/149‑1 (MQ=255)
                           gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCAGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAAGATGATGAATGGGGCGACCAGc                                                                                       >  1:555215/1‑149 (MQ=255)
                                   acAGAAAGGTGGAGCCGTAATACACACCGGCTCTAAAAGCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                               <  1:3220/148‑1 (MQ=11)
                                    cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACTGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGAGAGCATGATGAAAGGGGCAACCAGCTTAATgggg                                                                              >  2:38096/1‑149 (MQ=11)
                                    cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTAGGATGAATGGGGCAACCAGCTTCATgagg                                                                              >  2:578403/1‑149 (MQ=11)
                                     aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGTGAAACAAGCTTAAggagga                                                                             >  1:74037/1‑149 (MQ=255)
                                     aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                             >  1:420564/1‑149 (MQ=255)
                                       aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATCAATGGGGAAACCAACGTAATGAGgaga                                                                           >  1:190257/1‑149 (MQ=11)
                                       aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCTCGGAATATAAAGAATGGAGCAACCAGCTTTAtaataaaa                                                                           >  1:259419/1‑142 (MQ=17)
                                       aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAAACGGCAATACCGCGTGGCGCGGGGAGTAAGATGAATGGGGCAACCAGCTAAAAgaggaaa                                                                           >  1:190648/1‑147 (MQ=11)
                                           gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                       >  1:423245/1‑149 (MQ=255)
                                            tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGCGTATGATGAATGGGGCAACCAGCGTGAGGAGGAGAACCCg                                                                      >  2:555787/1‑149 (MQ=11)
                                              gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGTTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATAGGGAGAACCAGca                                                                    >  1:107352/1‑149 (MQ=11)
                                                   ggAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGACTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                               >  2:463110/1‑149 (MQ=255)
                                                          acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGAAGCGCGGAATATGAGGAGTCGGGCAACCAGCTCAATGAGGAGGACACGCGTCAACTGTAtac                                                        >  2:358508/1‑147 (MQ=17)
                                                            acCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCAGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAACGGGGCAACCGCCTCAATGAGGAGAACCCGCATCACGGGTATAAga                                                      >  2:339413/1‑148 (MQ=17)
                                                                gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTa                                                  >  2:114904/1‑149 (MQ=255)
                                                                gcgcGAAAAGATCATCTATATAAATGAGAAAACAGGGGACTGCAGGTGGAGATTATCAGCGAAGACGAAAATACGGAGTGGAGAGAGGAATATGATGAATGGGGCAAACAGCTTAATGAGGAGAACACGAATCATGTGTATCAGGCGTa                                                  >  2:371580/1‑149 (MQ=255)
                                                                   cgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCg                                               >  1:467137/1‑149 (MQ=255)
                                                                         gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                 >  1:369556/1‑93 (MQ=14)
                                                                         gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                 <  2:369556/93‑1 (MQ=11)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                             <  1:13251/111‑1 (MQ=11)
                                                                           tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                             >  2:13251/1‑111 (MQ=255)
                                                                                   aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGCGGAGCGCGGGATATGATGAATGGAGCAACCAGCTTAATGCGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcaa                               >  2:555434/1‑148 (MQ=17)
                                                                                      aCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                            <  1:114904/149‑1 (MQ=255)
                                                                                                 cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcag                                  >  2:337535/1‑132 (MQ=255)
                                                                                                 cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcag                                  <  1:337535/132‑1 (MQ=255)
                                                                                                        tGCCGCTGGCGCTTATCAGCGAAGACGGCACTCCGAAATGTCGCGGGGAGAATGATGAATGGGGCAAACAGCTTAATGAGGAGAAACCGCATCACCTGAACCAGACGTAACGTATGCAAGGGCAGCAGCAAGATGAGGAGTCGGGGCTg          >  1:591544/1‑149 (MQ=255)
                                                                                                                gcgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCTGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTCCTATaa  >  1:71290/1‑149 (MQ=255)
                                                                                                                                aCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                         <  1:73563/110‑1 (MQ=255)
                                                                                                                                aCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                         >  2:73563/1‑110 (MQ=255)
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GCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAA  >  NC_000913_3_bme_pgi/526647‑526907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑