Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	1,529,218	C→T	pseudogene (1317/2037 nt)	rhsE →	pseudogene, Rhs family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	1,529,218	0	C	T	100.0%	46.3 / NA	22	pseudogene (1317/2037 nt)	rhsE	pseudogene, Rhs family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

CACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACC  >  NC_000913_3_bme_pgi/1529160‑1529363                                                           |                                                                                                                                                  cacCGGCGCGAAAAGCTCATTTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAg                                                         <  1:480416/149‑1 (MQ=255)        gcgAAAAGCTCATCTTTGTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTAcc                                                  <  1:209599/149‑1 (MQ=11)          gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTTGCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGt                                                >  1:321817/1‑149 (MQ=11)          gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCCCCAGCCGTACCGt                                                >  1:218139/1‑149 (MQ=11)                 tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTACTGAGGAGAACCCGCATCACCTGCAACAGCCGTACCGTCTGCCAg                                         >  1:26893/1‑149 (MQ=11)                   atctttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGACGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAggg                                       >  2:34433/4‑149 (MQ=11)                     ctttATCACTGCGACCACCGGCGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGAAGAACCCGCATCACCTGCACCAGCCGTACCGTCCGCCAGGgca                                     >  2:527633/2‑149 (MQ=14)                              tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGCGTATGATGAAAGGGGCAACCAGCTTAATGAGAAGAACCCGCATCACCTGCACAAGCCGTACCGTCTGCCAGGGCAGTAGCatga                            >  2:478718/1‑149 (MQ=14)                               gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTACACCAGCCGTACCGTCTGCCAGGGCAGCAGCaagat                           >  1:50923/1‑149 (MQ=255)                                  accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgag                        >  1:176221/1‑149 (MQ=255)                                  accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgag                        <  2:433331/149‑1 (MQ=255)                                      ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATgaggaga                    >  1:205138/1‑148 (MQ=255)                                      ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCCTACCGTCTGCCACGGCAGCAGCATACTGAGGCGt                    >  2:166216/1‑149 (MQ=14)                                         gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGc                                            >  1:369429/1‑122 (MQ=255)                                         gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGc                                            <  2:369429/122‑1 (MQ=255)                                           gacTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCgggg               <  1:34433/147‑1 (MQ=255)                                            acTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAAGAGAACCCGCATCACCTGCACCAACCGTACCGTCTGCCAGGGCAGCAGCAGGAGGAGggggcggggc              >  2:321945/2‑149 (MQ=14)                                             gggCCGTTGGGGCTTATGAGCGAAGAGGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCt             <  2:50923/147‑1 (MQ=17)                                                 cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTAc         <  2:423245/149‑1 (MQ=255)                                                        gcTTATCCGCGATGACGGCAATACGGCGTGGCGCGTGGAGTATGATGAATTGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAAcc  <  1:228286/149‑1 (MQ=17)                                                        gcTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                     <  2:313027/50‑1 (MQ=255)                                                        gcTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                     >  1:313027/1‑50 (MQ=255)                                                           |                                                                                                                                                  CACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACC  >  NC_000913_3_bme_pgi/1529160‑1529363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑