| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 33,974 | C→A | A1053E (GCG→GAG) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 33,974 | 0 | C | A | 97.0% | 96.3 / NA | 33 | A1053E (GCG→GAG) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (17/15); minor base G (0/1); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTT > NC_000913_3_bme_pgi/33829‑34118 | aTCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCt < 1:637322/149‑1 (MQ=255) aGAATGCCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAAt < 1:249722/149‑1 (MQ=255) gCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCACGATTGAGCTGAGTGCGGAt > 1:108477/1‑149 (MQ=255) atatACCTACATCATCAAACCCACCTCAGGGCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGa < 2:55884/149‑1 (MQ=255) catcctCAACACCACCTCGGGCNCTCNTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAg < 1:634969/149‑1 (MQ=255) catcatCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAg > 1:204152/1‑149 (MQ=255) atcaACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAAt > 1:376575/1‑149 (MQ=255) atcaACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAAAGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAAt > 1:657288/1‑149 (MQ=255) tcaACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAAtt > 1:603801/1‑149 (MQ=255) caACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGACTTACGACACCACCCTGAACGGCGGCTTTGCTACTGCGATGGACCTGAATCCCAACGCGACTGAAAAAGTAAttt > 2:250134/1‑149 (MQ=255) ccaccTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTg > 1:686298/1‑149 (MQ=255) caccTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGGTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGATCTGAATGCGGATGCGAAAGAAAAAGTAATTTCGGTGc < 2:561880/149‑1 (MQ=255) cTCAGGCCGTGGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAgg > 1:814547/1‑149 (MQ=255) aGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGGGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGaaa < 1:57687/149‑1 (MQ=255) gCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACGACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGcaca < 2:742045/149‑1 (MQ=255) gcgcgTGATTCGTCGCAGTGCGCTGCAAGATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGc < 2:70723/148‑1 (MQ=255) ccGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGc < 1:428142/149‑1 (MQ=255) gcgTGATTCGCCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCATGAACGGCGGCTTTGCCACCGCGAGGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCgt < 1:99699/149‑1 (MQ=255) tGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATgca > 2:750916/1‑106 (MQ=255) tGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATgca < 1:750916/106‑1 (MQ=255) tGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATCTTTTTCAt > 1:212072/1‑149 (MQ=255) aaGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAAtt > 1:287245/1‑149 (MQ=255) aGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAAttt > 1:184389/1‑149 (MQ=255) aTTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCg > 2:663571/1‑149 (MQ=255) aCGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCAGCCGCTAATTTGATCGAAt < 2:603801/149‑1 (MQ=255) caccacCCTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACt < 1:780474/149‑1 (MQ=255) ccTGAACGGCGGCTTTGCCACCGCGATGGAGCTGTATGCCCATTCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACg > 1:411875/1‑149 (MQ=255) ccTGAACGGCGGCTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACg < 2:686298/149‑1 (MQ=255) ggcTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCtgat > 1:128966/1‑149 (MQ=255) gcTTTGCCACCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATCGCGACATCCGGCGGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCtgatg > 1:819922/1‑149 (MQ=255) aCCGCGATGGAGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGt > 2:528374/1‑149 (MQ=255) cgATGGAGGTGAATGCCGAAACGGGTGAAAAAGTAATTTCGGTGCATGAAAGGCACTCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGGTAATTAGATCTTAGAGCGAATACGGTTCTCTGATGAGGACCGttttt < 2:680352/149‑1 (MQ=255) aTGGGGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttt < 2:575852/149‑1 (MQ=255) | ATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACCCTGAACGGCGGCTTTGCCACCGCGATGGCGCTGAATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTT > NC_000913_3_bme_pgi/33829‑34118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |