| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,793 | C→A | intergenic (‑560/‑2114) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,793 | 0 | C | A | 98.1% | 150.0 / NA | 53 | intergenic (‑560/‑2114) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (26/26); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA > NC_000913_3_bme_pgi/4233649‑4233936 | gggTTTACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCACAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACAGTAGCaa > 2:232030/1‑149 (MQ=255) gTTTACACTCAACATTACGCTAACGGCCCCCACTCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTACTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAg > 2:391846/1‑149 (MQ=255) gTTTACACTCAACATGACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAg < 2:524610/149‑1 (MQ=255) tttACACTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGACGCTTGAAGCAACGGTAGCaagcc > 2:205867/1‑146 (MQ=255) cacTCAACATTACGCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGaa < 2:540508/149‑1 (MQ=255) gCTAACGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCGTGAAGCAACGATAGCAAAGCTGAAAAAGCATTGGGCg > 2:36610/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt > 2:785882/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt > 1:82942/1‑149 (MQ=255) aCGGCACCAAAACCATCACATTTTTCTGAGACTGGTGCCACCATCGTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGTCGAAGCTTGAAGCAACGGTAGCATAGCTGAACCAGCATTGGGCGGagt > 1:32544/1‑147 (MQ=255) ggCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCCACGCAGCTTGAAGCAACGATCGCAACGCTGAAAAAGCATGTGCCGGAATCg > 2:227051/1‑149 (MQ=255) gCACCAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCgg > 1:149871/1‑149 (MQ=255) accaaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCCCAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc < 1:224308/146‑1 (MQ=255) accaaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGACGAGTATTGCTAATGGCAACAGACGCGACGAAGCTTGAACCAACGGTAGCACACCCGAAAACGCATTGGCCGGAATCGGcc > 2:369109/4‑149 (MQ=255) caaaaCCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCAAAATTGTCTAAATCAGAAGAGTCTTGCTAATGGCACGAGACGCGACGAAGCTTGAAGCAACGGTAGCAGAGCTGAAAAAGCCTTGGTCGGAATCGGgccc > 2:151183/2‑149 (MQ=255) tttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGc < 1:16907/124‑1 (MQ=255) tttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGc > 2:16907/1‑124 (MQ=255) tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGACCATTAATGCGACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc > 1:405241/1‑149 (MQ=255) cTGGTGCTACAATCTTCCAAAGGCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg < 1:733702/149‑1 (MQ=255) cTGGTGCCACAATCTTCCAAAGTCACAATTTTCAAAATAAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg < 2:469033/149‑1 (MQ=255) tGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAgc > 2:691302/1‑149 (MQ=255) gaaTCTTCCAAAGTCACAATTCTCAACATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTCGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAccc < 2:169434/148‑1 (MQ=255) cAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAccc > 1:559620/1‑149 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGACAAAGCATTGGGCGCAATCGGCCCCGCGCGATATGCGCGCGGCCTTCAGCGCCGAcccc > 2:434281/1‑149 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAcccc > 2:781184/1‑149 (MQ=255) gttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc < 2:547276/148‑1 (MQ=255) cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGTCATTCAGCGCCGCACccggc > 2:25344/1‑149 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAAAGCCGACCCCGGCCGGtt > 2:758278/1‑149 (MQ=255) aaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc < 2:559620/149‑1 (MQ=255) tCAAAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg < 1:453637/149‑1 (MQ=255) cacaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc < 1:103315/149‑1 (MQ=255) acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCTGCGCCGACCCCCGCCGGTTCGGGCg > 2:462885/1‑149 (MQ=255) ctcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc < 2:437988/149‑1 (MQ=255) aagaGTATTGCTCATGGGGATAGGCACGACGAAGCTTGCAGAGCCGGCAGCAAAGCCAAAAATTCATCGTATGGTAGCTGCCCCGCGCGATATGCGGGCGGCATTCAGCGCCGACCCCGGCGGGTTCGGGCGATCTTAGCTCTCCTTgg > 2:177522/1‑149 (MQ=255) agagTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGga < 1:714398/149‑1 (MQ=255) tATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat > 1:512447/1‑149 (MQ=255) aTTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtc < 2:82942/149‑1 (MQ=255) cTAATGGCAAGAGACGGGACGAAGGTTGAAGCAACGGTAGCAAATCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGTGATATTCGCTCTGCCTGGATGAtcttct < 2:255841/149‑1 (MQ=255) cTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTATCACTGGATGAtcttct > 2:550048/1‑149 (MQ=255) cTAAGGGCAAGAGCCGCGACGAGGCTTGAAGGAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct < 2:304915/149‑1 (MQ=255) aaTGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATAGGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGt < 2:322925/149‑1 (MQ=255) aTGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt < 1:391846/149‑1 (MQ=255) agagACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCAGTGGGCCAAAACGGCCCCGCGCGAGATGCGTGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTg < 2:719076/149‑1 (MQ=255) gagaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTgg < 1:691302/149‑1 (MQ=255) aggcgcgAGGAAGCGTGAAGCAACGGTAGGAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt < 2:367599/146‑1 (MQ=255) gcgACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTAAACGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa < 1:785882/149‑1 (MQ=255) ttGAAGCAACGGTAGCAACGCTG‑AAACGCATTGGGCGGACTCCGCCCCGAGCGATATCCGCGCGCCCTTCAGCGCCGACCCCGGCCGGTGCGGGCGATATTCGCCCTGCCCGGGTTCTCTTCTGTTCGCCTGGTCGAAGTGcccgctca > 2:377198/1‑143 (MQ=255) ttGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGGCGCAGAGCCCGGta > 2:274620/1‑148 (MQ=255) gAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACGGGTAGAAGTGCCggggtga > 1:585501/1‑145 (MQ=255) aaGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAac < 1:781184/149‑1 (MQ=255) gCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacga < 1:364191/149‑1 (MQ=255) cAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacgac > 2:305205/1‑149 (MQ=255) aCGGTAGGAAGGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAAcgacga < 1:145453/149‑1 (MQ=255) aCGGTAGAAAAGCGTAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAAcgacga < 1:527178/149‑1 (MQ=255) | GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA > NC_000913_3_bme_pgi/4233649‑4233936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |