Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,177,1710TA20.5% 504.0 / 127.1 220intergenic (+43/‑187)tufB/secEtranslation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (82/92);  new base A (12/33);  total (95/125)
Fisher's exact test for biased strand distribution p-value = 1.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 234 total aligned reads displayed.

TCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTAT  >  NC_000913_3_bme_pgi/4177073‑4177268
                                                                                                  |                                                                                                 
 ccGTGAATGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                 >  2:5466719/1‑100 (MQ=255)
  cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                 >  1:1179608/1‑99 (MQ=255)
  cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                 >  1:4745726/1‑99 (MQ=255)
  cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                 <  1:5383797/99‑1 (MQ=255)
  cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGCAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTACAAGGGCATCAttt                                                                                                 >  2:4250396/1‑99 (MQ=255)
      aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                             <  1:4785999/99‑1 (MQ=255)
      aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGc                                                                                            <  1:283262/100‑1 (MQ=255)
        ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                          >  1:1524036/1‑100 (MQ=255)
        ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGcc                                                                                           >  2:2036460/1‑99 (MQ=255)
         gcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCt                                                                                         <  2:105933/100‑1 (MQ=255)
          cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                        <  2:1742553/100‑1 (MQ=255)
           ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttt                                                                                       <  1:2204746/100‑1 (MQ=255)
           ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttt                                                                                       <  1:5182940/100‑1 (MQ=255)
            gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCtttt                                                                                      <  1:1855999/100‑1 (MQ=255)
            gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                      <  1:879167/100‑1 (MQ=255)
            gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                      <  1:3245671/100‑1 (MQ=255)
            gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttt                                                                                       <  2:4332441/99‑1 (MQ=255)
             ccgtaccgtaGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                      <  2:5517648/99‑1 (MQ=38)
             ccGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt                                                                                     <  1:16843/100‑1 (MQ=255)
              cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                    <  1:708320/100‑3 (MQ=38)
              cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                    <  1:4766694/100‑3 (MQ=38)
              cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                    <  1:3354346/100‑3 (MQ=38)
              cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACAAAAAGGGCATCAAATGATGCCCttttag                                                                                    <  1:3719162/100‑3 (MQ=38)
               gTACCGTTGGCGCGGGCGTTGTATCAAAAGTTCTGAGCTAATTGCCGTTAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttac                                                                                    <  2:5104708/99‑3 (MQ=38)
               gTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagt                                                                                   <  1:1290143/100‑4 (MQ=38)
                tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagtg                                                                                  <  2:4965178/100‑5 (MQ=38)
                  ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                >  1:576351/1‑100 (MQ=255)
                    gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCt                                                                              <  1:3150411/100‑1 (MQ=255)
                    gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCt                                                                              >  2:2479126/1‑100 (MQ=255)
                         cgcgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCg                                                                          >  1:3207457/1‑99 (MQ=255)
                         cgcgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCg                                                                          >  2:2765114/1‑99 (MQ=255)
                           cgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGtn                                                                        <  2:2729214/99‑2 (MQ=255)
                           cgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAc                                                                       >  2:2943860/1‑100 (MQ=255)
                            gggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc                                                                      <  1:5330490/100‑1 (MQ=255)
                            gggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAc                                                                       >  1:1679569/1‑99 (MQ=255)
                            gagCGTTGTAGCAAAAGTTTTGAGCTAATTGCCTCTAACATTGTACGCAATGCGCACTAAAAGGACATCATTTGATGCCCTTTTTGCATGCTTTCGAAcc                                                                      <  2:4619015/98‑1 (MQ=255)
                             ggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCa                                                                     <  1:4190694/100‑1 (MQ=255)
                              gCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAc                                                                       >  2:1326900/1‑97 (MQ=255)
                              gCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAg                                                                    <  2:3478257/100‑1 (MQ=255)
                              gCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATCCGCACTAAAAGGGCATCACTTGATGCCCTTTTTGCACGCTTTCGTAc                                                                       >  2:739242/1‑97 (MQ=255)
                                    tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCt                                                               <  1:4372853/99‑1 (MQ=255)
                                    tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                              <  1:488288/100‑1 (MQ=255)
                                     aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGGACCTgg                                                             >  1:2654750/1‑100 (MQ=255)
                                     aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                             >  2:3654138/1‑100 (MQ=255)
                                     aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                              >  2:3500319/1‑99 (MQ=255)
                                     aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                              >  2:369630/1‑99 (MQ=255)
                                      gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                            <  1:2622363/100‑1 (MQ=255)
                                      gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                            <  2:149077/100‑1 (MQ=255)
                                      gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                            <  1:4446367/100‑1 (MQ=255)
                                      gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                            <  1:3588788/100‑1 (MQ=255)
                                      gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                            <  1:2978189/100‑1 (MQ=255)
                                           aGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcat                                                        >  2:1586381/1‑99 (MQ=255)
                                            gTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcat                                                        >  1:2534266/1‑98 (MQ=255)
                                             ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAg                                                     >  2:2252584/1‑100 (MQ=255)
                                                 gAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAt                                                 >  2:1129382/1‑100 (MQ=255)
                                                   gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                               >  1:1817400/1‑100 (MQ=255)
                                                   gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                               <  1:2809924/100‑1 (MQ=255)
                                                     tAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATtctc                                             >  2:2650007/1‑100 (MQ=255)
                                                     tAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTc                                             >  1:5273155/1‑100 (MQ=255)
                                                      aaTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCCGAACCTGGCTCATCAGTGCTTTTCt                                            >  2:3047089/1‑100 (MQ=255)
                                                      aaTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCt                                            >  1:4978340/1‑100 (MQ=255)
                                                      aaTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCt                                            >  1:574859/1‑100 (MQ=255)
                                                       aTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGCACCAGAACCTGGCTCATCAGTGATTTTCtt                                           <  2:3521756/100‑1 (MQ=255)
                                                        ttGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCttt                                          <  2:4181361/100‑1 (MQ=255)
                                                        ttGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCttt                                          <  1:4932061/100‑1 (MQ=255)
                                                         tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                         >  2:5167979/1‑100 (MQ=255)
                                                           ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                         <  2:1874324/98‑1 (MQ=255)
                                                           ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                        <  2:4020954/99‑1 (MQ=255)
                                                           ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                        <  1:1080569/99‑1 (MQ=255)
                                                           ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAGGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                       <  2:852695/100‑1 (MQ=255)
                                                            cGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCa                                      >  2:3494059/1‑100 (MQ=255)
                                                              aTAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATa                                    <  1:816267/100‑1 (MQ=255)
                                                               tAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATaa                                   >  2:2364872/1‑100 (MQ=255)
                                                                 aCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATc                                 <  1:752373/100‑1 (MQ=255)
                                                                   aTTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAt                               >  1:3767637/1‑100 (MQ=255)
                                                                    tttGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAtt                              >  1:721773/1‑100 (MQ=255)
                                                                      tGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                            >  2:3325573/1‑100 (MQ=255)
                                                                       gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                            <  1:3954150/99‑1 (MQ=255)
                                                                       gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                           >  1:855813/1‑100 (MQ=255)
                                                                         cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCATAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTgn                         <  1:4126950/100‑2 (MQ=255)
                                                                         cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                         <  1:1900115/100‑1 (MQ=255)
                                                                          gCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTgag                        <  1:1994743/100‑1 (MQ=255)
                                                                            aaTGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGAc                      <  1:1631188/100‑1 (MQ=255)
                                                                                cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                  <  2:1184319/100‑1 (MQ=255)
                                                                                  cACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAGCTGGCTCATCATTGATTTTCTTTGTCATAATGATTGCTGAGACTGGctc                >  1:4625417/1‑100 (MQ=255)
                                                                                  cACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctc                >  1:511885/1‑100 (MQ=255)
                                                                                   gcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct               >  1:548904/4‑100 (MQ=39)
                                                                                     aaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGt             >  1:4701539/2‑100 (MQ=255)
                                                                                      aaaaGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct               >  2:3863743/1‑97 (MQ=255)
                                                                                       aaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt            >  2:5391509/1‑99 (MQ=255)
                                                                                       aaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt            >  1:3058539/1‑99 (MQ=255)
                                                                                        aaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt            >  2:487269/1‑98 (MQ=255)
                                                                                         acggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                  <  2:3114812/89‑1 (MQ=255)
                                                                                         aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAg         >  1:4010675/1‑100 (MQ=255)
                                                                                         aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAg         <  1:788744/100‑1 (MQ=255)
                                                                                           ggCATCGTTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg       <  2:4691500/100‑1 (MQ=255)
                                                                                           ggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg       <  2:2070603/100‑1 (MQ=255)
                                                                                             catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCg     <  1:46742/100‑1 (MQ=255)
                                                                                                 atTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtat  <  1:3113531/99‑1 (MQ=255)
                                                                                                 atTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGta   >  2:1636979/1‑98 (MQ=255)
                                                                                                  |                                                                                                 
TCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTAT  >  NC_000913_3_bme_pgi/4177073‑4177268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: