Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,778,3670CG22.3% 442.4 / 143.7 206intergenic (+20/+23)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (99/61);  new base G (29/17);  total (128/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 221 total aligned reads displayed.

AACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTAC  >  NC_000913_3_bme_pgi/1778268‑1778466
                                                                                                   |                                                                                                   
aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttc                                                                                                     >  2:562740/1‑100 (MQ=255)
 aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttct                                                                                                    <  2:2401766/100‑1 (MQ=255)
   gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTa                                                                                                  <  1:3546705/100‑1 (MQ=255)
     aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTaa                                                                                                 >  1:4289657/1‑99 (MQ=255)
     aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTaa                                                                                                 >  1:334704/1‑99 (MQ=255)
     aTCGGCGGCGGTAAAGACGGAATTATGACTTACGTAGCAGTTCGTCCTATCCTGAAAGACCATCAGAACAAATAATCTGCACGCGTCGCAGATTCCTaa                                                                                                 >  2:1110249/1‑99 (MQ=255)
      tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                                >  2:3087625/1‑99 (MQ=255)
      tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                                <  1:553470/99‑1 (MQ=255)
      tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                                <  1:2117109/99‑1 (MQ=255)
      tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACa                                                                                               <  2:1190988/100‑1 (MQ=255)
       cggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAGAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACa                                                                                               >  1:4160424/1‑99 (MQ=255)
        ggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGaa                                                                                              <  1:29144/99‑1 (MQ=255)
        ggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACaaa                                                                                             >  1:3245813/1‑100 (MQ=255)
              ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                       >  2:4043999/1‑100 (MQ=255)
              ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                       <  1:2064251/100‑1 (MQ=255)
              ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                       <  1:1371315/100‑1 (MQ=255)
               gTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                      >  1:499806/1‑100 (MQ=255)
               gTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                      >  2:688751/1‑100 (MQ=255)
                tACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgccg                                                                                     <  2:322661/100‑1 (MQ=38)
                tACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgccg                                                                                     <  1:230545/100‑1 (MQ=38)
                  cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcc                                                                                   <  1:3773245/100‑1 (MQ=38)
                  cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc                                                                                    <  1:4505249/99‑1 (MQ=255)
                  cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgcc                                                                                   <  1:3418642/100‑1 (MQ=255)
                   aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcct                                                                                  <  1:2379251/100‑2 (MQ=37)
                   aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcct                                                                                  <  1:193058/100‑2 (MQ=37)
                    gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcctg                                                                                 <  1:1278885/100‑3 (MQ=37)
                    gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc                                                                                    >  1:1791962/1‑97 (MQ=255)
                    gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccgc                                                                                    >  2:578460/1‑97 (MQ=255)
                      cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAt                                                                               >  1:2796782/1‑100 (MQ=255)
                       gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                              <  2:2115004/100‑1 (MQ=255)
                       gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                              >  1:1146164/1‑100 (MQ=255)
                       gAAATTATGATTTACGTAGCAGGTCGGCAGAGCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                              <  2:2615416/100‑1 (MQ=255)
                           ttATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATc                                                                          >  1:2372391/1‑100 (MQ=255)
                           ttATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATc                                                                          >  1:2803974/1‑100 (MQ=255)
                              tGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaa                                                                       >  1:2719246/1‑100 (MQ=255)
                              tGATTTACGTAGCAGGTCGGCAGACCCTGAAAGACTATCAGAACAAATAATCTGCCGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTCTCcaa                                                                       >  2:3748944/1‑100 (MQ=255)
                               gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcac                                                                       >  2:4183421/1‑98 (MQ=255)
                               gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaa                                                                       >  1:790060/1‑99 (MQ=255)
                               gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTAACcaa                                                                       >  2:4121661/1‑99 (MQ=255)
                                  ttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac                                                                      >  2:2798567/1‑97 (MQ=255)
                                     cGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGAc                                                                 <  2:3968157/99‑1 (MQ=255)
                                       tAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                              >  2:5051541/1‑100 (MQ=255)
                                          cAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAcc                                                            <  2:1438393/99‑1 (MQ=255)
                                           aGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATCTATCCACCAAGACTTACCgg                                                          >  2:914025/1‑100 (MQ=255)
                                                ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                     >  1:4910420/1‑100 (MQ=255)
                                                        cTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAtt                                               >  1:4758987/1‑98 (MQ=255)
                                                          gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAtt                                               >  2:2160675/1‑96 (MQ=255)
                                                             aGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAg                                        >  2:814270/1‑100 (MQ=255)
                                                              gACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                       <  1:994859/100‑1 (MQ=255)
                                                              gACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                       <  1:2825046/100‑1 (MQ=255)
                                                              gACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAGTTATCGAACAAGACTTACCGGATGAg                                                     >  2:4709064/1‑86 (MQ=255)
                                                                  aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAttt                                   >  1:1558390/1‑100 (MQ=255)
                                                                   tCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAtt                                    <  2:1704696/98‑1 (MQ=255)
                                                                   tCAGAAAAAATAATCTGCAGACGGCGCAGCCTCTTAACACACTGCGCCGCCAGATTTATcc                                                                         <  2:1548873/61‑1 (MQ=255)
                                                                     aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTCTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt                                 >  2:3359545/1‑99 (MQ=255)
                                                                     aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCtt                                <  2:2412529/100‑1 (MQ=255)
                                                                      gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                               <  1:1679216/100‑1 (MQ=255)
                                                                      gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                               <  2:3672175/100‑1 (MQ=255)
                                                                         cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTCCCGGTTGAGGCAATTCAGCTAGGATTTATTGTTc                            >  2:5038705/1‑100 (MQ=255)
                                                                         cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                            >  1:3866195/1‑100 (MQ=255)
                                                                         cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                            >  2:3349167/1‑100 (MQ=255)
                                                                           aataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCg                          <  1:3509664/100‑1 (MQ=255)
                                                                            ntaatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCg                          >  1:54234/2‑99 (MQ=255)
                                                                            ataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCg                          >  1:4987641/1‑99 (MQ=255)
                                                                               atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaa                       >  2:1286268/1‑99 (MQ=255)
                                                                               atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaa                       >  2:1127224/1‑99 (MQ=255)
                                                                               atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaa                       <  2:4393892/99‑1 (MQ=255)
                                                                                  tGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGtt                                                        >  2:1790597/1‑63 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                    <  1:58773/97‑1 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                    <  1:2217345/97‑1 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                    <  1:5081585/97‑1 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGt                 >  1:4402246/1‑100 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGt                 >  1:3988853/1‑100 (MQ=255)
                                                                                    cAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGt                 >  1:2584908/1‑100 (MQ=255)
                                                                                     tggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                >  1:3121518/2‑100 (MQ=37)
                                                                                     tggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                >  2:1989059/2‑100 (MQ=37)
                                                                                      ggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTGCCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt               >  2:884903/1‑100 (MQ=37)
                                                                                       gcggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  1:503665/1‑100 (MQ=38)
                                                                                        cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  2:1585685/1‑99 (MQ=38)
                                                                                        cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  2:2430853/1‑99 (MQ=38)
                                                                                        cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  2:4796919/1‑99 (MQ=38)
                                                                                        cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  2:1353179/1‑99 (MQ=38)
                                                                                        cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtttt              >  1:1104370/1‑99 (MQ=38)
                                                                                        cggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             <  2:4867509/100‑1 (MQ=255)
                                                                                         ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  1:3704511/1‑99 (MQ=38)
                                                                                         ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  1:3067640/1‑99 (MQ=38)
                                                                                         ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  1:3186547/1‑99 (MQ=38)
                                                                                         ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  2:3865599/1‑99 (MQ=38)
                                                                                         ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  1:4669530/1‑99 (MQ=38)
                                                                                         ggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  1:317096/1‑99 (MQ=255)
                                                                                           cgcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt             >  2:1806430/1‑97 (MQ=255)
                                                                                           cgcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa          >  2:4251142/1‑100 (MQ=255)
                                                                                             cAGCTTCTTAACAAACTGCGCCGCAAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTa            <  2:2956851/96‑1 (MQ=255)
                                                                                             cAGCTTCTTAACAAAATGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGAAAATTCAGATAGGATTTCTTGTTCCGCAAGACAGttt               >  2:5191509/1‑93 (MQ=255)
                                                                                                cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTg       <  2:3904888/98‑1 (MQ=255)
                                                                                                cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGt      >  2:2117762/1‑99 (MQ=255)
                                                                                                cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc     >  2:1755659/1‑100 (MQ=255)
                                                                                                 ttcttcACAAACTGCGCCGCCAGATTTATCCAACAAGACGCACCGGCTCAGGAAAATCTGATAGGACTTCTAGTTCCGCaaaa                     >  2:4649522/1‑83 (MQ=255)
                                                                                                   cttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc     >  2:2253005/1‑97 (MQ=255)
                                                                                                   cttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTAc  >  1:4808326/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTAC  >  NC_000913_3_bme_pgi/1778268‑1778466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑