Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,950,2920CG20.4% 404.8 / 106.4 178A77A (GCG→GCCaspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (67/73);  new base G (22/14);  total (91/87)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 184 total aligned reads displayed.

CGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCA  >  NC_000913_3_bme_pgi/1950196‑1950389
                                                                                                 |                                                                                                  
cGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCa                                                                                                 <  1:767972/100‑1 (MQ=255)
 ggCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCa                                                                                                 <  2:1461365/99‑1 (MQ=255)
  gCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACg                                                                                              <  1:4799393/100‑1 (MQ=255)
    aGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTa                                                                                            <  1:5066653/100‑1 (MQ=255)
     gAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTAc                                                                                           >  1:3807122/1‑100 (MQ=255)
       aCATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACgg                                                                                         <  2:1718679/100‑1 (MQ=255)
        cATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGt                                                                                        >  1:1368095/1‑100 (MQ=255)
           cTGAGCGGTTGATGATAGTCAGCGAGGACGCCAGCTCTTCGATTACGCCGGTCGCCATATCGCGGCGAATATTTTTTTCGCCACGCGCACGTCCGGTgac                                                                                     >  2:4430605/1‑98 (MQ=255)
              cgcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGccc                                                                                    <  1:1863547/98‑1 (MQ=255)
              cgcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGccc                                                                                    <  2:1844894/98‑1 (MQ=255)
              cgcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGcc                                                                                     <  2:1502526/97‑1 (MQ=255)
              cgcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCg                                                                                   <  2:2662691/99‑1 (MQ=255)
              cgcgGTTGATGATAGTCAGCGAGGACGCCAGCACTGCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGt                                                                                  <  2:3595270/100‑1 (MQ=255)
               gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGt                                                                                  >  2:3922894/1‑99 (MQ=255)
               gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGt                                                                                  >  2:3743143/1‑99 (MQ=255)
                cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                 <  1:4278579/99‑1 (MQ=255)
                cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                 <  2:73124/99‑1 (MQ=255)
                cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGa                                                                                <  1:1811756/100‑1 (MQ=255)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAc                                                                               <  1:3751942/100‑1 (MQ=38)
                 ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAc                                                                               <  1:1012636/100‑1 (MQ=38)
                  gTTGATGATAGTCAGCGGGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAcc                                                                              <  2:4990087/100‑1 (MQ=255)
                  gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAc                                                                               <  2:4508574/99‑1 (MQ=255)
                  gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGATGCCCGTGAcc                                                                              >  2:3406263/1‑100 (MQ=255)
                  gTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTCAATATTTTTTTCGTCACGGTCACCGTACGTGCGCGTGAc                                                                               <  1:4892189/99‑1 (MQ=255)
                   ttGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcga                                                                             <  1:3295370/100‑3 (MQ=37)
                    tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCt                                                                             >  2:1799604/1‑99 (MQ=255)
                     gatgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTg                                                                            >  1:1728073/1‑99 (MQ=255)
                     gatgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGGCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcgaa                                                                            <  1:4062898/99‑4 (MQ=37)
                      atgatAGTCAGCGAGGGCGCCAGCACGTCGATTTCGCCGGGCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGGGCGCGTGAcgaaaa                                                                          <  2:2817349/100‑6 (MQ=25)
                      atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcgaaa                                                                           <  1:374338/99‑5 (MQ=37)
                      atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcgaaa                                                                           <  1:1547283/99‑5 (MQ=37)
                      atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTGTCGTCACGCGCACGTACGGTGCCCGTGAc                                                                               <  2:1502118/95‑1 (MQ=255)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAAt                                                                         >  1:2133471/1‑100 (MQ=255)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAAt                                                                         >  1:2404116/1‑100 (MQ=255)
                       tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAAt                                                                         >  1:346290/1‑100 (MQ=255)
                         atAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGc                                                                       <  2:2577005/100‑1 (MQ=255)
                          tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCa                                                                      <  1:4129102/100‑1 (MQ=255)
                          tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCa                                                                      <  1:262410/100‑1 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                     >  2:2309956/1‑99 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                     >  1:1268785/1‑99 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                     >  1:1526134/1‑99 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                     >  1:4604269/1‑99 (MQ=255)
                            gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAg                                                                     <  1:1654758/99‑1 (MQ=255)
                             tCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGa                                                                    <  1:2603663/99‑1 (MQ=255)
                               aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                  <  1:3277551/99‑1 (MQ=255)
                                 cGAGGGCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTACGTGCGCGTGAcgaaa                                                                           <  2:4297280/88‑5 (MQ=21)
                                 cGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                               >  2:4764104/1‑100 (MQ=255)
                                   aGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACt                                                                 >  2:3519413/1‑96 (MQ=255)
                                    ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCAt                                                              >  2:4799371/1‑98 (MQ=255)
                                        gCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCa                                                        <  1:3990142/100‑1 (MQ=255)
                                         ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                       >  2:1606319/1‑100 (MQ=255)
                                           aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGtt                                                     >  1:3855710/1‑100 (MQ=255)
                                              aCTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCag                                                  <  2:3121772/100‑1 (MQ=255)
                                               cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCa                                                   >  1:92403/1‑98 (MQ=255)
                                                  cGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAgg                                               <  1:3567126/99‑1 (MQ=255)
                                                  cGATTTCGCCGGTCGCCATAACGCGGTTAATATTTTTTTTCGTCACGAGCACGTAAGGTGCCCGTGACCTGAACGCAGGACTCATTACGCAGTTCACAgg                                               >  2:3253560/1‑100 (MQ=255)
                                                     tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                           <  1:3345076/100‑1 (MQ=255)
                                                       tCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGc                                          <  1:864868/99‑1 (MQ=255)
                                                         gCCGGTCGCCATATCGCGGTTAATATTTTTGTCGGCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                 <  2:1439381/90‑1 (MQ=255)
                                                          ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCttt                                       >  1:439497/1‑99 (MQ=255)
                                                          ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                      <  1:1251433/100‑1 (MQ=255)
                                                            ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                     >  1:4023620/1‑99 (MQ=255)
                                                             gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcg                                   <  1:5000641/100‑1 (MQ=255)
                                                             gTCGCCATATCGCGGTTAATATTTTTTCCGTCACGCGCCCGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcg                                   >  1:4052050/1‑100 (MQ=255)
                                                                gCCATATCGCGGTTAATATTTTTTTTTTTTTTTTTCCGTACGGAGCCCGTGACCCGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTCACGCGt                                >  2:1145143/1‑100 (MQ=255)
                                                                gCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                 <  1:1004543/99‑1 (MQ=255)
                                                                gCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGAGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGt                                >  1:5276951/1‑100 (MQ=255)
                                                                 ccATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGt                                >  2:4607545/1‑99 (MQ=255)
                                                                 ccATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACCGTGCCCGTGACCTGAATGCAGAACTCATTACGCCGTTCAGAGGCCAGCCTTAACGCGtt                               >  2:3218259/1‑99 (MQ=255)
                                                                  cATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTcc                              >  1:3884936/1‑100 (MQ=255)
                                                                  cATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTcc                              >  1:2358096/1‑100 (MQ=255)
                                                                    tatCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCg                             <  2:1722966/99‑1 (MQ=255)
                                                                      tCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCAc                          <  2:414275/100‑1 (MQ=255)
                                                                        gcgGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGa                        >  1:5234157/1‑100 (MQ=255)
                                                                           gTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATcc                     >  1:4073840/1‑100 (MQ=255)
                                                                            ttAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCg                    >  2:533338/1‑100 (MQ=255)
                                                                              aaTATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGa                  >  2:832057/1‑100 (MQ=255)
                                                                                tatTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                <  1:4619548/100‑1 (MQ=255)
                                                                                   tttttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg               <  1:1298831/99‑1 (MQ=255)
                                                                                   tttttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg               <  1:2903591/99‑1 (MQ=255)
                                                                                    ttttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCCGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGa              >  2:2197299/1‑99 (MQ=255)
                                                                                      ttttCGTCACGCGCACGTACGGTGCCCGTGACCTGGATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg               <  2:1087263/96‑1 (MQ=255)
                                                                                      ttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa            >  1:4498798/1‑99 (MQ=255)
                                                                                      ttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaa            <  1:2068831/99‑1 (MQ=255)
                                                                                        caggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaaa          >  1:655200/4‑99 (MQ=37)
                                                                                        caggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaaa          >  2:683869/4‑99 (MQ=37)
                                                                                          ggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc       >  2:3856293/2‑100 (MQ=38)
                                                                                          ggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc       >  1:956099/2‑100 (MQ=38)
                                                                                          ggTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc       >  1:1526381/2‑100 (MQ=38)
                                                                                          ggTCACGGGCACCGTACGTGCGCGTGACCTGAATCCAGCACTCACTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGCTCGAAAAATAc       >  2:1896040/2‑100 (MQ=25)
                                                                                           gTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc      >  2:2254110/1‑100 (MQ=38)
                                                                                           gTCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc      >  1:2667867/1‑100 (MQ=38)
                                                                                           gTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc      >  1:3077202/1‑100 (MQ=255)
                                                                                            tCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt     >  1:544844/1‑100 (MQ=38)
                                                                                             cACGGGCACCGTACGTGCGCGTGACCTGAATGCATAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg    >  1:5141943/1‑100 (MQ=255)
                                                                                             cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg    >  1:3183533/1‑100 (MQ=255)
                                                                                             cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg    >  1:3914409/1‑100 (MQ=255)
                                                                                                gcgcACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGGACGAGCCGGATc                <  2:4196976/85‑1 (MQ=255)
                                                                                                gcgcACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc      <  2:2829142/95‑1 (MQ=255)
                                                                                                gcgcACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa  <  2:4696059/99‑1 (MQ=255)
                                                                                                 |                                                                                                  
CGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCA  >  NC_000913_3_bme_pgi/1950196‑1950389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: