| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,772 | G→T | intergenic (‑539/‑2135) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,772 | 0 | G | T | 95.0% | 113.9 / ‑6.0 | 40 | intergenic (‑539/‑2135) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (22/16); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGG > NC_000913_3_bme_pgi/4233630‑4233913 | acaTGCTTCACTGCGCTAAGGGTCTACACTCAACATTACGCTACCGTCCGCGAGTCGATCACATTTTTCTGTGACTGGCGCTACAATGTTCCAGATTCATAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAg < 1:343118/147‑1 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTCCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTGAAAATCAGACGGGGATTGCCAATCGCCACAGTCGCTACGACGc > 2:531458/1‑149 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGc < 1:198059/149‑1 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAATATTGCTAATGGCAAGAGACGCTACGAGGc > 1:143300/1‑149 (MQ=255) tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAAc < 1:422745/149‑1 (MQ=255) cgcTAAGGCTTTACACTCAATATGAGGCTAAATGTACTAATACCATCACATTTTTCTGTGACTTGCGTTACAATCTTCCAGAGTCACATTTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACgg < 2:620743/149‑1 (MQ=255) acacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGa > 2:279623/1‑149 (MQ=255) cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGTTTGAAGCAACGGTCGCAAAGCTGaa > 2:625075/1‑149 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaa > 1:621016/1‑149 (MQ=255) cgaaaaTTACGCTAACGGAACTAAACCCACCACATTTGTCTGTGACTGACGCTAAAATCTTCCAAAGTCACAACTCTCAAAGTCAGAAGAGTATTACAAAATTCAGGATACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa < 2:165040/144‑1 (MQ=255) aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 1:413446/1‑149 (MQ=255) aaTTACGCTAACGGCACTAAAACCATCACATTTTTCTGGGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACTCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAt < 2:325417/148‑1 (MQ=255) aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt < 1:626081/149‑1 (MQ=255) tACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg < 2:198539/149‑1 (MQ=255) cGCTAACGGCACTAAAACCAGCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGc < 1:547516/149‑1 (MQ=255) gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAAGCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg > 2:707738/1‑149 (MQ=255) cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGccc < 2:319224/149‑1 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc > 2:55096/1‑149 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc > 1:386619/1‑149 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc > 1:293105/1‑149 (MQ=255) aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg > 2:589049/1‑149 (MQ=255) caTTTTTCTGGGACTGGCTCTAAAATCTTCCAAAGTCACAATTCTCCAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCGGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc < 2:609901/149‑1 (MQ=255) aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGATCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGGTATgcg > 2:3377/1‑149 (MQ=255) gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGTCCCCGCGCGATATGCGCGCGGCATTCAgcgc > 1:368600/1‑149 (MQ=255) aTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCCCGATATGCGCGCGGCATTCAGCGCCGaaaccg > 2:192949/1‑144 (MQ=255) tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccgg > 2:554784/1‑149 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGCAACAACAACTGGTGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt > 1:757466/1‑149 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt > 2:3947/1‑149 (MQ=255) caATTCTCACAAGCAGAAGAGTATTGCCAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGa < 1:356280/149‑1 (MQ=255) ttATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatga > 2:331538/2‑149 (MQ=255) tATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat < 1:707738/149‑1 (MQ=255) tATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat < 2:143300/149‑1 (MQ=255) tGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt < 1:279623/149‑1 (MQ=255) tAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTg > 1:690434/1‑149 (MQ=255) tAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTg > 1:105105/1‑149 (MQ=255) gcaaagtaCGCTACGAAGCTTGAATCAACGGTCGCAAAGCCGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCTCGCGGCATTCAGCGCCGACCCCTGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa < 2:448777/142‑1 (MQ=255) gCAAGAGGCGCTACGAAGCTTGAAGCAACCGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa < 2:654480/149‑1 (MQ=255) gCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGAAAAGCCGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa > 2:132495/1‑149 (MQ=255) cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGAc < 1:554784/149‑1 (MQ=255) gagaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGGTATTCGCTCTGCCTGGATGATCTTCTGTTCGACTgg > 1:256299/1‑149 (MQ=255) | AAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGG > NC_000913_3_bme_pgi/4233630‑4233913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |