Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,7960CG20.5% 85.0 / 17.3 40A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (15/16);  new base G (2/6);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGG  >  NC_000913_3_bme_pgi/526649‑526941
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ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                   <  1:391259/149‑1 (MQ=255)
ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                   >  2:391259/1‑149 (MQ=255)
tggcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGGGTATTGTCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                   <  2:379872/147‑1 (MQ=17)
         gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATa‑tgat                                                                                                                                         <  2:253215/149‑1 (MQ=255)
             gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAACAGCTCATCTTTATAAATAAGACCACCGGGGACTGCCGCTGGCGCTTGTCAGCGAAGACGGCAGTACGGCGTGGAGCGCGGCAtg‑tgctatat                                                                                                                                     >  2:148696/1‑143 (MQ=14)
             gCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAAt                                                                                                                                     <  1:255320/149‑1 (MQ=11)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGATGAAGATTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATgg                                                                                                                                   <  1:368673/149‑1 (MQ=11)
               tGACGGCGGAGCAACTGGCCAGACACGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTGTATCACTGCGACCACCACGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATACGGGGTGGCGCGGGGAGTA‑TGATGAATgg                                                                                                                                   <  1:477749/149‑1 (MQ=11)
               cgACGGTGGAGGAACGGGCCTGACAGGGGGGGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                   <  2:280531/148‑1 (MQ=17)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATggg                                                                                                                                  <  1:180740/149‑1 (MQ=255)
                gACGGCGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAGAGCTCATCTTTATCAACGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGACAATACGGCGTGGCGCGGGGAGTA‑TGATGAATggg                                                                                                                                  <  2:69540/149‑1 (MQ=11)
                         gaaaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                   <  2:304010/137‑1 (MQ=255)
                         gaaaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATgg                                                                                                                                   >  1:304010/3‑139 (MQ=255)
                           aaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCtt                                                                                                                       >  2:460373/1‑149 (MQ=255)
                            aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGTGGCAACCAGCTTa                                                                                                                      >  1:219290/1‑149 (MQ=255)
                             cTGGCCAGACAGGTGGAGACGGAATACACACCGGCGCGAATAGCTCATTATTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTaa                                                                                                                     <  2:55789/149‑1 (MQ=255)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                  >  1:228745/1‑149 (MQ=255)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                  <  1:3762/147‑1 (MQ=255)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATga                                                                                                                  >  2:3762/1‑147 (MQ=255)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATgagga                                                                                                               >  1:362007/1‑149 (MQ=255)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATgagga                                                                                                               >  2:415063/1‑149 (MQ=255)
                                    gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGAATCCCCGCGGCCCCTCTCAGAGACGGAGGCAATCCGTCCTGACGCG‑GGAGGACTGCTGAATGGGGCAACCAGCGTGGTgaggga                                                                                                              >  2:85540/1‑147 (MQ=2)
                                       aGGTGTAGCCAGAATACATACTCGCGCGAAAAGCTAATCTATATAACTGCGAACAGCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGTCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                           <  2:192789/149‑1 (MQ=17)
                                                cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                  <  1:280965/149‑1 (MQ=255)
                                                  gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGCA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcaacacca                                                                                                >  2:432369/1‑146 (MQ=11)
                                                     tACACACCGGCGCGAAAAGCTCATCTTGATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgca                                                                                             <  2:353834/149‑7 (MQ=1)
                                                      gcacacCGGCGCGAAAACCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                            <  2:333792/148‑1 (MQ=255)
                                                       cacccCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                           <  2:142039/149‑1 (MQ=255)
                                                       cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                           <  1:415063/149‑1 (MQ=255)
                                                        accccGGCGGGAGAAGCTCATCTTTATCATTGCGACCACCGGGGACTGGCGCTGGCGGTTATCAGCGAAGACGGCAATACGTCGTGGAGCTCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                          <  2:305426/146‑1 (MQ=17)
                                                          acCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTTTATCGGc                                                                                        >  1:17764/1‑149 (MQ=255)
                                                                         tCATCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                         <  1:121645/149‑1 (MQ=255)
                                                                           aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                       >  1:151650/1‑149 (MQ=255)
                                                                                   cACTGCGACCACCGGGGACTCCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGACTA‑TGATGAATCGGGCAACCAGCTTAATGAGGAGCACCCGCATCATGTGTATCCGCCTGACCGCCTCCCCGGccagcatca                                                               >  2:315856/1‑149 (MQ=14)
                                                                                            caccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                      >  1:218964/1‑149 (MQ=255)
                                                                                                 gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                 <  1:288743/149‑1 (MQ=255)
                                                                                                   gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                               <  1:460373/149‑1 (MQ=255)
                                                                                                      tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGACAA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                            >  1:498333/1‑149 (MQ=255)
                                                                                                        ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                          >  1:351908/1‑149 (MQ=255)
                                                                                                              gcgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                    >  2:340924/1‑149 (MQ=255)
                                                                                                                     tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                             >  2:394546/1‑149 (MQ=255)
                                                                                                                       aGCGAAGACGGCAATACGGCGTGGAGCGCTGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                           <  2:219290/149‑1 (MQ=255)
                                                                                                                                  cAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                <  2:218964/149‑1 (MQ=255)
                                                                                                                                                gcgcGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCgg  >  2:311264/1‑149 (MQ=255)
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TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATA‑TGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGG  >  NC_000913_3_bme_pgi/526649‑526941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑