New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 3345001	16 (0.510)	3 (0.100)	3/268	4.3	15.0%	coding (285/1434 nt)	rpoN	RNA polymerase, sigma 54 (sigma N) factor
	?	NC_000913_3_bme_pgi	= 3345013	19 (0.650)					coding (297/1434 nt)	rpoN	RNA polymerase, sigma 54 (sigma N) factor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACC  >  NC_000913_3_bme_pgi/3344892‑3345142                                                                                                              |                                                                                                                                              gcgAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTgccgtcagg                                                                                                        <  2:204927/149‑6 (MQ=255)             aCAGGGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTAc                                                                                            <  2:342751/149‑1 (MQ=255)                   aaaCGCCGGACACCGCCCACGCGATCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCCCCAGCGGTGACTACATTGTCGACGAGCTGCCGGTCTACCAGGGc                                                                                      <  2:424342/149‑1 (MQ=255)                                 gccgACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGAc                                                                        <  1:319598/149‑1 (MQ=255)                                 gccgACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGAc                                                                        <  1:503069/149‑1 (MQ=255)                                   cgACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCTGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCt                                                                      <  2:544642/149‑1 (MQ=255)                                                 aaaaaGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCCGGGCGAACCGACGCAGACCTTGCGGGATTACCTg                                                        >  1:157053/1‑149 (MQ=255)                                                     agagATGCCGTAAGAGCGGCCGCTCGATGCCAGTTGGGACACCACTTACGCCGCTGGTACACCAGCCGGCACCAGCGGTGACTACATTGACGACCAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGAtgt                                                    <  1:209659/149‑1 (MQ=255)                                                      gagaTGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGAtgtg                                                   >  1:333437/1‑149 (MQ=255)                                                              ggAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTg                                           >  1:327191/1‑149 (MQ=255)                                                                aaGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAg                                         >  1:276631/1‑149 (MQ=255)                                                                     cTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCGGGCACCAACGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGGGAAACGACGACGACCTTGCAGGATTGCCTGATGTGGCGGGGTGAGCTgtg                                    >  1:14761/1‑147 (MQ=255)                                                                                     gTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCCGGGCCAATCGTCGCGGACCGTGCCGCTGCTGCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACt                    <  2:525122/149‑1 (MQ=255)                                                                                                 tttACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTAtt        >  1:383799/1‑149 (MQ=255)                                                                                                      acCGCTGGTACACAATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTAc   >  1:446924/1‑149 (MQ=255)                                                                                                       ccGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTAcc  <  1:246511/149‑1 (MQ=255)                                                                                                              |                                                                                                                                              GCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGACGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTTCCGACACTGACCGCGCTATTGCTACC  >  NC_000913_3_bme_pgi/3344892‑3345142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.