Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,7960CG37.7% 57.2 / 58.9 53A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (15/18);  new base G (7/13);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGC  >  NC_000913_3_bme_pgi/526651‑526939
                                                                                                                                                 |                                                                                                                                               
gcgcAGTGCGGGCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGTAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                                                              <  2:536739/149‑1 (MQ=255)
      tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                        <  1:285602/149‑1 (MQ=255)
          ggCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGCATACACAGCGGCGCGAAAAGCTCATCTTTATCACTGCGATCACCGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATAGGGCGTGGCGCGGGGAGTATGATGaa                                                                                                                                    <  1:414731/149‑1 (MQ=11)
             tGACGGGGGACCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                 <  1:172656/149‑1 (MQ=11)
              gaccggggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                <  1:139803/143‑1 (MQ=11)
              gACCGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACAGCCGCCGCCGCTTATCATCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                <  2:500010/149‑1 (MQ=11)
                          aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCGGGCGTGGCGCGGGGAGTGTGATGAATGGGGAAACCAGATTa                                                                                                                    >  1:20717/1‑149 (MQ=11)
                           cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                       <  2:277183/129‑1 (MQ=255)
                           cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                       >  1:277183/1‑129 (MQ=255)
                           cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                   <  1:168880/133‑1 (MQ=255)
                           cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                   >  2:168880/1‑133 (MQ=255)
                            tGGCCAGCCAGGTGGCGCCGACTTACACAGCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                  <  1:11029/149‑1 (MQ=11)
                              gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAACGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                <  1:17696/149‑1 (MQ=255)
                              gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                <  2:44292/149‑1 (MQ=255)
                               ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCGCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                               <  2:9577/149‑1 (MQ=255)
                                cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgggg                                                                                                              >  1:20284/1‑149 (MQ=255)
                                    cgggTGGGGCGGGAAGACACACGGGCGCGAAAAGCTCATCTTTATCACTGCGAGCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGaa                                                                                                          <  2:151598/147‑1 (MQ=11)
                                     aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                         >  1:218538/1‑149 (MQ=255)
                                       gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAGTATGATGAACGGGGCCACCAGCTTAATGAGGAGAAccc                                                                                                       >  1:365288/1‑149 (MQ=255)
                                            gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                  >  1:254400/1‑149 (MQ=255)
                                            gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGACTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcacc                                                                                                  >  2:20364/1‑147 (MQ=255)
                                              cGGAATACACACTGGCGCGAAATGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGGGAAGACGGCAAGACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                <  2:325691/149‑2 (MQ=11)
                                              cGGAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                <  2:268787/149‑1 (MQ=255)
                                               ggAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGGGCGGAGAGTATGATGAATGGGGCAACCAGCTTAATTAGGAGAACCCgactcccc                                                                                               >  1:159467/1‑142 (MQ=11)
                                                    acacacCGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                          <  1:162985/149‑8 (MQ=11)
                                                         ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGGAACCGCATCATGTGTATCAGcc                                                                                     >  2:492395/1‑149 (MQ=255)
                                                         ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGcc                                                                                     <  2:321576/149‑1 (MQ=255)
                                                              gcgAAAAGCTCATCTTTATCACTGCTACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACTGCAATACGGCGTGGAGACCGGAATATGATGAATGGGGGAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                <  1:492395/149‑1 (MQ=18)
                                                                gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATAATGTGTATCAGCCGTACCGt                                                                              >  1:239778/1‑149 (MQ=255)
                                                                  aaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                            <  2:58455/149‑1 (MQ=255)
                                                                     gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                           >  2:403576/1‑99 (MQ=11)
                                                                     gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                           <  1:403576/99‑1 (MQ=11)
                                                                      cTCATCTTTATCACTGCGACCACCGGGATGTGCGGGTGCAGCCTCTCAGACCAGACCGCAATACGGCGTGGAGCTCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                        <  1:261153/149‑1 (MQ=17)
                                                                       tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                       >  1:226552/1‑149 (MQ=255)
                                                                         atcgtgaTCACTGCGACCACCGCGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAGGGGGCAACCAGCTTAATGAGGAGAACCCGAATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                     <  2:392053/143‑1 (MQ=17)
                                                                         aTCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCCGCGAAGACGGCAATACGACGCGGCGCGGGGAGAATGAGGAACGGGCCAACCAGCTTAATCAGGAGAACCCACATCAACTGCACCACCCGTACCGCCTGCCAtgg                                                                     >  2:442800/1‑149 (MQ=255)
                                                                         aTCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                     <  2:84028/149‑1 (MQ=255)
                                                                           cTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTGTCAGCCGTACCGGCTGCCCGGgca                                                                   >  2:245044/1‑149 (MQ=255)
                                                                               aTCACTGCGACCACGGGGGACTGCAGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                               >  1:472017/1‑149 (MQ=255)
                                                                               aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAAACGGCAATACGGCGGGGAGCTCGGAATATGATGAATGGGGCAACCAGCTTAAGGAGGAGAACCCGCACCATGTGTATCAGCCGTACAGTCTCCCAGGgcagcag                                                               >  2:116148/1‑149 (MQ=17)
                                                                               aTCACCGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                               <  1:233866/149‑1 (MQ=255)
                                                                                tCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATAATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagc                                                              >  1:256674/1‑149 (MQ=255)
                                                                                           cccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAACACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGaa                                                   <  1:232462/148‑1 (MQ=255)
                                                                                            ccGGGGACTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGATAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAt                                                  <  1:108233/149‑1 (MQ=255)
                                                                                                    tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCGGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                          >  1:159463/1‑149 (MQ=255)
                                                                                                      ccGCTGGCGCTGAGCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGGACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                        <  2:14088/149‑1 (MQ=255)
                                                                                                              gcTTATCCGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                <  1:500233/149‑1 (MQ=255)
                                                                                                              gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                <  1:377448/149‑1 (MQ=255)
                                                                                                                ttATCAGCGAAGACGGCAATACGGGGTGGAGGGCGGAATATGATGAATGGGACAACCAGCTTAATGAGGAGAATCCGCATCATCTGTATCAGCCGTACCGTCTGCCTAGGCAGGAGTATGATGAGGAATCAGGGTTGTACTATAACCGt                              <  2:517782/149‑1 (MQ=17)
                                                                                                                      gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATACCGGTCACCgg                        >  2:350538/1‑149 (MQ=255)
                                                                                                                        gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCggga                      >  2:356354/1‑147 (MQ=255)
                                                                                                                              ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGCATCAGGGCTGTACTATCACCACCACCGGcactacaa                >  2:292015/1‑147 (MQ=17)
                                                                                                                                            gAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGc  <  1:116148/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGC  >  NC_000913_3_bme_pgi/526651‑526939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑