Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi526,8000AG38.5% 52.8 / 46.2 53E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (13/19);  new base G (8/12);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_bme_pgi/526657‑526946
                                                                                                                                               |                                                                                                                                                  
tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                               <  1:285602/149‑1 (MQ=255)
    ggCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGCATACACAGCGGCGCGAAAAGCTCATCTTTATCACTGCGATCACCGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATAGGGCGTGGCGCGGGGAGTATGATGaa                                                                                                                                           <  1:414731/149‑1 (MQ=11)
       tGACGGGGGACCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                        <  1:172656/149‑1 (MQ=11)
        gaccggggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                       <  1:139803/143‑1 (MQ=11)
        gACCGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACAGCCGCCGCCGCTTATCATCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                       <  2:500010/149‑1 (MQ=11)
                    aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCGGGCGTGGCGCGGGGAGTGTGATGAATGGGGAAACCAGATTa                                                                                                                           >  1:20717/1‑149 (MQ=11)
                     cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                              <  2:277183/129‑1 (MQ=255)
                     cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                              >  1:277183/1‑129 (MQ=255)
                     cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                          >  2:168880/1‑133 (MQ=255)
                     cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                          <  1:168880/133‑1 (MQ=255)
                      tGGCCAGCCAGGTGGCGCCGACTTACACAGCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                         <  1:11029/149‑1 (MQ=11)
                        gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAACGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                       <  1:17696/149‑1 (MQ=255)
                        gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                       <  2:44292/149‑1 (MQ=255)
                         ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCGCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                      <  2:9577/149‑1 (MQ=255)
                          cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgggg                                                                                                                     >  1:20284/1‑149 (MQ=255)
                              cgggTGGGGCGGGAAGACACACGGGCGCGAAAAGCTCATCTTTATCACTGCGAGCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGaa                                                                                                                 <  2:151598/147‑1 (MQ=11)
                               aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                >  1:218538/1‑149 (MQ=255)
                                 gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAGTATGATGAACGGGGCCACCAGCTTAATGAGGAGAAccc                                                                                                              >  1:365288/1‑149 (MQ=255)
                                      gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                         >  1:254400/1‑149 (MQ=255)
                                      gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGACTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcacc                                                                                                         >  2:20364/1‑147 (MQ=255)
                                        cGGAATACACACTGGCGCGAAATGCTCATCTTTATCACTGCGGCCACCGGGGACTGCCGCTGGCGCTTATCAGGGAAGACGGCAAGACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                       <  2:325691/149‑2 (MQ=11)
                                        cGGAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                       <  2:268787/149‑1 (MQ=255)
                                         ggAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGGGCGGAGAGTATGATGAATGGGGCAACCAGCTTAATTAGGAGAACCCgactcccc                                                                                                      >  1:159467/1‑142 (MQ=11)
                                              acacacCGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                                 <  1:162985/149‑8 (MQ=11)
                                                   ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGGAACCGCATCATGTGTATCAGcc                                                                                            >  2:492395/1‑149 (MQ=255)
                                                   ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGcc                                                                                            <  2:321576/149‑1 (MQ=255)
                                                        gcgAAAAGCTCATCTTTATCACTGCTACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACTGCAATACGGCGTGGAGACCGGAATATGATGAATGGGGGAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                       <  1:492395/149‑1 (MQ=18)
                                                          gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATAATGTGTATCAGCCGTACCGt                                                                                     >  1:239778/1‑149 (MQ=255)
                                                            aaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                                   <  2:58455/149‑1 (MQ=255)
                                                               gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                  <  1:403576/99‑1 (MQ=11)
                                                               gCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                                  >  2:403576/1‑99 (MQ=11)
                                                                cTCATCTTTATCACTGCGACCACCGGGATGTGCGGGTGCAGCCTCTCAGACCAGACCGCAATACGGCGTGGAGCTCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                               <  1:261153/149‑1 (MQ=17)
                                                                 tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                              >  1:226552/1‑149 (MQ=255)
                                                                   atcgtgaTCACTGCGACCACCGCGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAGGGGGCAACCAGCTTAATGAGGAGAACCCGAATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                            <  2:392053/143‑1 (MQ=17)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCCGCGAAGACGGCAATACGACGCGGCGCGGGGAGAATGAGGAACGGGCCAACCAGCTTAATCAGGAGAACCCACATCAACTGCACCACCCGTACCGCCTGCCAtgg                                                                            >  2:442800/1‑149 (MQ=255)
                                                                   aTCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                                            <  2:84028/149‑1 (MQ=255)
                                                                     cTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTGTCAGCCGTACCGGCTGCCCGGgca                                                                          >  2:245044/1‑149 (MQ=255)
                                                                         aTCACTGCGACCACGGGGGACTGCAGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                                      >  1:472017/1‑149 (MQ=255)
                                                                         aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAAACGGCAATACGGCGGGGAGCTCGGAATATGATGAATGGGGCAACCAGCTTAAGGAGGAGAACCCGCACCATGTGTATCAGCCGTACAGTCTCCCAGGgcagcag                                                                      >  2:116148/1‑149 (MQ=17)
                                                                         aTCACCGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                                      <  1:233866/149‑1 (MQ=255)
                                                                          tCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATAATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagc                                                                     >  1:256674/1‑149 (MQ=255)
                                                                                     cccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAACACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGaa                                                          <  1:232462/148‑1 (MQ=255)
                                                                                      ccGGGGACTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGATAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAt                                                         <  1:108233/149‑1 (MQ=255)
                                                                                              tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCGGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                                 >  1:159463/1‑149 (MQ=255)
                                                                                                ccGCTGGCGCTGAGCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGGACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                               <  2:14088/149‑1 (MQ=255)
                                                                                                        gcTTATCCGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                       <  1:500233/149‑1 (MQ=255)
                                                                                                        gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                       <  1:377448/149‑1 (MQ=255)
                                                                                                          ttATCAGCGAAGACGGCAATACGGGGTGGAGGGCGGAATATGATGAATGGGACAACCAGCTTAATGAGGAGAATCCGCATCATCTGTATCAGCCGTACCGTCTGCCTAGGCAGGAGTATGATGAGGAATCAGGGTTGTACTATAACCGt                                     <  2:517782/149‑1 (MQ=17)
                                                                                                                gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATACCGGTCACCgg                               >  2:350538/1‑149 (MQ=255)
                                                                                                                  gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCggga                             >  2:356354/1‑147 (MQ=255)
                                                                                                                        ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGCATCAGGGCTGTACTATCACCACCACCGGcactacaa                       >  2:292015/1‑147 (MQ=17)
                                                                                                                                      gAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGc         <  1:116148/149‑1 (MQ=255)
                                                                                                                                             gAATAGGATGAATGGGGCAAACAGCGGAAGGAGGAAAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGGACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTatat  <  1:222995/149‑1 (MQ=17)
                                                                                                                                               |                                                                                                                                                  
TGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATAT  >  NC_000913_3_bme_pgi/526657‑526946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑