Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,233,708 C→T intergenic (‑475/‑2199) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,233,7080CT98.7% 238.5 / NA 76intergenic (‑475/‑2199)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (44/31);  minor base G (0/1);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAG  >  NC_000913_3_bme_pgi/4233562‑4233852
                                                                                                                                                  |                                                                                                                                                
gggCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGgtgc                                                                                                                                                <  2:770791/149‑1 (MQ=255)
      ttGTCAACGATTGGGTGATGACGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAAt                                                                                                                                          <  1:1031663/149‑1 (MQ=255)
      ttGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAAt                                                                                                                                          <  2:369863/149‑1 (MQ=255)
        gTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCt                                                                                                                                        >  1:397922/1‑149 (MQ=255)
         tCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATAACGCTAACGGCACTAAAACAATCACATTTTTCTGTGACTGGTGCTACAATCtt                                                                                                                                       >  1:1124551/1‑149 (MQ=255)
            aCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCa                                                                                                                                    >  1:1356703/1‑149 (MQ=255)
                tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGt                                                                                                                                <  1:743080/149‑1 (MQ=255)
                 ggggTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCCCTGCGCTAAGGGGTGACCCTCCACCTTCCGCTGACGGCACTAAAGCCATCACATTTTTCTGTGCCTGGTGCGACAATCGTCCaacggc                                                                                                                               >  2:831283/1‑145 (MQ=255)
                       aTGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACCCTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAAtt                                                                                                                         >  1:708214/1‑149 (MQ=255)
                          cGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc                                                                                                                      <  2:1164309/149‑1 (MQ=255)
                          cGGATTATTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATtctc                                                                                                                      <  2:1403953/149‑1 (MQ=255)
                            gATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCaa                                                                                                                    >  2:278550/1‑149 (MQ=255)
                                tttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATc                                                                                                                >  1:403734/1‑149 (MQ=255)
                                 ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTGAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                               <  2:1082945/149‑1 (MQ=255)
                                 ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                               <  1:180207/149‑1 (MQ=255)
                                 ttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCa                                                                                                               <  1:1098534/149‑1 (MQ=255)
                                    aTCCACTCGTGGCGGTAAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACGCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGACACAATTCTCAAAATCagaa                                                                                                            <  1:980470/149‑1 (MQ=255)
                                    aTCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAAAGCTTCACTGAGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAAGCTTCAAAAGTCACAATTCTCAAAATCagaa                                                                                                            >  2:707792/1‑149 (MQ=255)
                                      ccACTCCTGGCGGGCTGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaaga                                                                                                          <  2:424709/149‑1 (MQ=255)
                                      ccACTCCGGGCGGTCAGTAGTTCACCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaaga                                                                                                          <  2:316197/149‑1 (MQ=255)
                                        aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGt                                                                                                        >  1:1202224/1‑149 (MQ=255)
                                              ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTACGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACTTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTCTTGCt                                                                                                  <  2:528468/149‑1 (MQ=255)
                                              ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCt                                                                                                  >  1:1050500/1‑149 (MQ=255)
                                              ggCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTAAGCTAACTGAACTAAAACAATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGACACAATTATCAAAATCAGAAGATTATTGCt                                                                                                  >  1:98812/1‑149 (MQ=255)
                                                 ggTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAAt                                                                                               <  1:860535/149‑1 (MQ=255)
                                                  gTCCGTAGTTCAGCTAATAAATGCTAAACTGCGCTAAGGGTTGACACTCAACATAACGCTAACGGCACTAAATCCCTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAATTCCCAATTCTCAAAATCAGAAGAGTATTGCTAATg                                                                                              <  2:820837/149‑1 (MQ=255)
                                                   tCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGATTACACTCAACATTACGCTAACGGCACTAAAACCATCACAATTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAATATCAGAAGAGTATTGCTAATgg                                                                                             <  1:902375/149‑1 (MQ=255)
                                                     agtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCa                                                                                           <  2:16290/149‑1 (MQ=255)
                                                         gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAagag                                                                                       <  2:573551/149‑1 (MQ=255)
                                                          ttCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAgaga                                                                                      >  2:736927/1‑149 (MQ=255)
                                                              gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTGCAATCGGCCAAAGTCAGAATTCTGAAAGTCAGATGAGTATTGGTAATGGCGAGAGAcgcg                                                                                  >  2:1268683/1‑149 (MQ=255)
                                                              gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGAcgcg                                                                                  >  2:1169656/1‑149 (MQ=255)
                                                               cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGcga                                                                                 <  1:278550/149‑1 (MQ=255)
                                                                        gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTg                                                                        <  1:580208/149‑1 (MQ=255)
                                                                         cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa                                                                       <  1:1322309/149‑1 (MQ=255)
                                                                         cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa                                                                       <  1:242304/149‑1 (MQ=255)
                                                                         cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGa                                                                       >  1:187110/1‑149 (MQ=255)
                                                                          ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTTACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                                      <  1:1245222/149‑1 (MQ=255)
                                                                          ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGCCTGGTGCTACAATCTTCCAAAGTCACAATTCTGAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                                      >  2:1071661/1‑149 (MQ=255)
                                                                             aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCa                                                                   >  2:531087/1‑149 (MQ=255)
                                                                             aCTGCGCTAAGGGTTTACACTCAACATTACGCAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCaa                                                                  >  1:648872/1‑149 (MQ=255)
                                                                              cTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACACTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACCCGACGAAGCTTGAAGCaa                                                                  >  1:69624/1‑149 (MQ=255)
                                                                                 cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAAc                                                                 >  2:530361/1‑147 (MQ=255)
                                                                                 cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAAc                                                                 <  1:530361/147‑1 (MQ=255)
                                                                                 cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACg                                                                <  2:945054/148‑1 (MQ=255)
                                                                                 cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACg                                                                >  1:945054/1‑148 (MQ=255)
                                                                                   cTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTACTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTc                                                             >  1:442306/1‑149 (MQ=255)
                                                                                     aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTcac                                                           <  2:25148/149‑3 (MQ=255)
                                                                                     aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGGGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTcac                                                           <  2:118076/149‑3 (MQ=255)
                                                                                         gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                       >  1:1477300/1‑149 (MQ=255)
                                                                                         gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                       <  2:1050500/149‑1 (MQ=255)
                                                                                         gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTGTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                       <  1:232889/149‑1 (MQ=255)
                                                                                         gTTAACACTCAACATTACGCTAACGGCACTAAAACGATCACATTTTTCTGTGACTGCTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACTTTCACAAAg                                                       <  2:98812/149‑1 (MQ=255)
                                                                                          tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGc                                                      >  1:308401/1‑149 (MQ=255)
                                                                                                cTCAACATTACGCTAACGGCTCTAAAACCATCTCATTTTTCTGTGACTGGTGCTACTAGCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaa                                                >  2:792924/1‑149 (MQ=255)
                                                                                                cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaa                                                >  1:767441/1‑149 (MQ=255)
                                                                                                 tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAACGCTGaaaaa                                               >  2:917267/1‑149 (MQ=255)
                                                                                                 tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                               >  1:756340/1‑149 (MQ=255)
                                                                                                 tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                               >  1:617369/1‑149 (MQ=255)
                                                                                                 tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGAGACTGGTGCTACAAACTTCCAAAGGCACAATTCTCAAAATCAGACGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                               <  2:711674/149‑1 (MQ=255)
                                                                                                   aaCATTACGCTAACGGCACTTAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGc                                             >  2:1323787/1‑149 (MQ=255)
                                                                                                                cGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATc                                >  1:172653/1‑149 (MQ=255)
                                                                                                                 ggCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCg                               >  1:1164879/1‑149 (MQ=255)
                                                                                                                  gCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTTATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCgg                              >  2:376765/1‑149 (MQ=255)
                                                                                                                             aTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                   >  2:714402/1‑149 (MQ=255)
                                                                                                                               cacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat                 >  2:1268080/1‑149 (MQ=255)
                                                                                                                                 caTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCATAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc               >  2:1392559/1‑149 (MQ=255)
                                                                                                                                  aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg              >  2:1457006/1‑149 (MQ=255)
                                                                                                                                  aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg              >  1:1085084/1‑149 (MQ=255)
                                                                                                                                   tttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc             >  1:278063/1‑149 (MQ=255)
                                                                                                                                   tttttCTATGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCAAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc             >  2:1359605/1‑149 (MQ=255)
                                                                                                                                    ttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg            <  1:1323787/149‑1 (MQ=255)
                                                                                                                                     tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc           >  1:748760/1‑149 (MQ=255)
                                                                                                                                            gACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCACCGGTCACAAAGCTGAAAAAGCATTGGGCGGATTCGGCCCCGCGCGATAGGCGCGCGGCATTc    >  2:1370690/1‑149 (MQ=255)
                                                                                                                                            gACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTc    >  1:21450/1‑149 (MQ=255)
                                                                                                                                              cTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg  >  1:443127/1‑149 (MQ=255)
                                                                                                                                              cTGGTGCTACAATCATCCAAAGTCACTATTCTCAAATTCAGAAGAGTACTGCTAATGGCTAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAATCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg  <  2:403734/149‑1 (MQ=255)
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GGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAG  >  NC_000913_3_bme_pgi/4233562‑4233852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: