Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,233,794	G→A	intergenic (‑561/‑2113)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,233,794	0	G	A	100.0%	303.1 / NA	97	intergenic (‑561/‑2113)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (60/37);  total (60/37)

GTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA  >  NC_000913_3_bme_pgi/4233651‑4233936                                                                                                                                                |                                                                                                                                               gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                                                                                                           <  2:1050500/149‑1 (MQ=255) gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                                                                                                           >  1:1477300/1‑149 (MQ=255) gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTGTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAg                                                                                                                                           <  1:232889/149‑1 (MQ=255) gTTAACACTCAACATTACGCTAACGGCACTAAAACGATCACATTTTTCTGTGACTGCTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACTTTCACAAAg                                                                                                                                           <  2:98812/149‑1 (MQ=255)  tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGc                                                                                                                                          >  1:308401/1‑149 (MQ=255)        cTCAACATTACGCTAACGGCTCTAAAACCATCTCATTTTTCTGTGACTGGTGCTACTAGCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaa                                                                                                                                    >  2:792924/1‑149 (MQ=255)        cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaa                                                                                                                                    >  1:767441/1‑149 (MQ=255)         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAACGCTGaaaaa                                                                                                                                   >  2:917267/1‑149 (MQ=255)         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                                                                                                                   >  1:617369/1‑149 (MQ=255)         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                                                                                                                   >  1:756340/1‑149 (MQ=255)         tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGAGACTGGTGCTACAAACTTCCAAAGGCACAATTCTCAAAATCAGACGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGaaaaa                                                                                                                                   <  2:711674/149‑1 (MQ=255)           aaCATTACGCTAACGGCACTTAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGc                                                                                                                                 >  2:1323787/1‑149 (MQ=255)                        cGGCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                                                    >  1:172653/1‑149 (MQ=255)                         ggCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCg                                                                                                                   >  1:1164879/1‑149 (MQ=255)                          gCACTAAAACCATCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTTATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                                                  >  2:376765/1‑149 (MQ=255)                                     aTCACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGat                                                                                                       >  2:714402/1‑149 (MQ=255)                                       cacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat                                                                                                     >  2:1268080/1‑149 (MQ=255)                                         caTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCATAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc                                                                                                   >  2:1392559/1‑149 (MQ=255)                                          aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                                  >  1:1085084/1‑149 (MQ=255)                                          aTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcg                                                                                                  >  2:1457006/1‑149 (MQ=255)                                           tttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc                                                                                                 >  1:278063/1‑149 (MQ=255)                                           tttttCTATGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCAAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc                                                                                                 >  2:1359605/1‑149 (MQ=255)                                            ttttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcg                                                                                                <  1:1323787/149‑1 (MQ=255)                                             tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgcgc                                                                                               >  1:748760/1‑149 (MQ=255)                                                    gACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCACCGGTCACAAAGCTGAAAAAGCATTGGGCGGATTCGGCCCCGCGCGATAGGCGCGCGGCATTc                                                                                        >  2:1370690/1‑149 (MQ=255)                                                    gACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTc                                                                                        >  1:21450/1‑149 (MQ=255)                                                      cTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg                                                                                      >  1:443127/1‑149 (MQ=255)                                                      cTGGTGCTACAATCATCCAAAGTCACTATTCTCAAATTCAGAAGAGTACTGCTAATGGCTAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAATCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAg                                                                                      <  2:403734/149‑1 (MQ=255)                                                          tgcTACACGCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCGGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGcc                                                                                  <  2:708214/148‑1 (MQ=255)                                                             tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAc                                                                               >  1:448603/1‑149 (MQ=255)                                                              aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAcc                                                                              >  1:222648/1‑149 (MQ=255)                                                              aCAAACTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGTCAAGAGACGAGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGAGCGCGGCATTCATCGCCGAcc                                                                              >  1:801391/1‑149 (MQ=255)                                                                aaTATTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGTCGGAATCGGCCCAGCGCGATATGCGCTCGGCATTCAGCGACGAcccc                                                                            >  2:1210300/1‑149 (MQ=255)                                                                  tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccgg                                                                          >  2:1146162/1‑149 (MQ=255)                                                                   tttacAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTATTGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc                                                                         <  1:707792/145‑1 (MQ=255)                                                                    ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggcc                                                                        <  2:1477300/149‑1 (MQ=255)                                                                     tCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggccg                                                                       <  2:1356703/149‑1 (MQ=255)                                                                        aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                                    >  1:573949/1‑149 (MQ=255)                                                                        aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                                    >  2:230102/1‑149 (MQ=255)                                                                        aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGAGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                                                    >  1:1101537/1‑149 (MQ=255)                                                                        aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGAAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCAGACCCCGGCCGGtt                                                                    >  1:157276/1‑149 (MQ=255)                                                                              acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg                                                              >  2:1194229/1‑149 (MQ=255)                                                                              acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg                                                              >  2:1144005/1‑149 (MQ=255)                                                                                aaTTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGat                                                            >  2:1132238/1‑149 (MQ=255)                                                                                         aaccagcagAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAACGCATTGGGCGGAATCGGCGCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                                                   <  1:912746/146‑1 (MQ=255)                                                                                         aaTCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                                                   <  1:531087/149‑1 (MQ=255)                                                                                           tCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCACCGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGACCCGCGCGATATGCGCGCGGCAGTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                                                 >  1:52548/1‑149 (MQ=255)                                                                                           tCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                                                 <  1:1144005/149‑1 (MQ=255)                                                                                           tCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc                                                 <  2:1085084/149‑1 (MQ=255)                                                                                           tCACAAGAGTATTGCTAATCGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGACTCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctcttc                                                 <  2:1202224/149‑3 (MQ=255)                                                                                             agaagaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCt                                               >  1:334483/1‑149 (MQ=255)                                                                                             agaagaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCt                                               <  2:187110/149‑1 (MQ=255)                                                                                               aagaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTgg                                             >  2:1251522/1‑149 (MQ=255)                                                                                                 gagTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCTGTTCGGGCGATATTCGCTCTGCCTGgat                                           >  1:1415547/1‑149 (MQ=255)                                                                                                 gagTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTTgat                                           <  2:1124551/149‑1 (MQ=255)                                                                                                 gagTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgat                                           >  2:417725/1‑149 (MQ=255)                                                                                                  agTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatg                                          >  1:4195/1‑149 (MQ=255)                                                                                                     aTTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtc                                       <  2:1164879/149‑1 (MQ=255)                                                                                                       tGCTAATGGCAAGAGACGCGACGAAGCTTGATGCAAGGGGCACAAAGCTGAAAAAGCATTGGGCGGTATCGGCGCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                                     <  2:397922/149‑1 (MQ=255)                                                                                                       tGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt                                     <  1:1457006/149‑1 (MQ=255)                                                                                                         cTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct                                   <  1:1180574/149‑1 (MQ=255)                                                                                                         cTAATGGCAAGAGACCCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct                                   <  1:792924/149‑1 (MQ=255)                                                                                                           aaTGGCAAGAGACGCGACGAAGCTTGAAGCCACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGACATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGt                                 <  2:172653/149‑1 (MQ=255)                                                                                                            aTGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATTCGCGCGGCATTCAGCGCCGACCCCGGCCGGGTCGGGCGATATTAGCTCTGCCTGGAAGAACTTCTGtt                                >  2:151682/1‑149 (MQ=255)                                                                                                            aTGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt                                <  2:648872/149‑1 (MQ=255)                                                                                                              ggCAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCGTCTGTTCg                              >  1:1097587/1‑149 (MQ=255)                                                                                                               gCAAGAGACCCGCCGAAGCATGCAGCAACGGTCACAAAGCAGCAAAAGCCTTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGa                             <  2:442306/149‑1 (MQ=255)                                                                                                                cAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGGGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGAc                            <  1:376765/149‑1 (MQ=255)                                                                                                                cAAGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGAc                            >  1:464957/1‑149 (MQ=255)                                                                                                                 aaGAGACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACt                           >  1:127455/1‑149 (MQ=255)                                                                                                                  agagACGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCTATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTGCGACTg                          >  2:760167/1‑149 (MQ=255)                                                                                                                     gaCGCGACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTc                       <  2:617369/149‑1 (MQ=255)                                                                                                                        gcgACGAAGCTTGACGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGTAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    <  2:69624/149‑1 (MQ=255)                                                                                                                        gcgACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    >  2:767411/1‑149 (MQ=255)                                                                                                                        gcgACGAAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    >  2:344299/1‑149 (MQ=255)                                                                                                                        gcgACGAAGCTGGAAGCAACGGTCACAAAGCTGAAAAAGCAATCGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa                    <  1:831283/149‑1 (MQ=255)                                                                                                                         cgacgaAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAg                   <  1:1169656/149‑1 (MQ=255)                                                                                                                          gacgaAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGACGGGATGATCTTCTGTTCGAATGGTCGAAGt                  >  2:701618/1‑149 (MQ=255)                                                                                                                             gaAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGcc               >  2:910699/1‑149 (MQ=255)                                                                                                                             gaAGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGcc               >  2:388987/1‑149 (MQ=255)                                                                                                                              aaGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCg              <  1:417725/149‑1 (MQ=255)                                                                                                                               aGCTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCgg             <  1:1268080/149‑1 (MQ=255)                                                                                                                                 cTTGAAGCAACGGTCATAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGt           <  1:1392559/149‑1 (MQ=255)                                                                                                                                 cTTGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGt           >  1:1411535/1‑149 (MQ=255)                                                                                                                                  ttGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGATTCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGGCTGGATGATCGTCTGTTGGAATGGTCGAAGTGCCGGGtt          >  2:1129073/1‑149 (MQ=255)                                                                                                                                  ttGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGtt          >  2:1242517/1‑149 (MQ=255)                                                                                                                                  ttGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCGGCCTGGATGATCTTCTGGTCGACTGGTCGAAGTGCCGGGtt          >  2:1134455/1‑149 (MQ=255)                                                                                                                                   tGAAGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCCGCGCCGACCCCGGCCGGTTCGGGCGAGATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTa         >  2:579080/1‑149 (MQ=255)                                                                                                                                    gAAGCCACGGTCACAAAGCTGAAAAAGCATAGGGCGGAATCGGCCCCGCGCGATCTGCGCGCGGCATTCAGCGCCGACCCCGGGCGGTTCGGGCGATATTCGCTCTGCCAGGATGATCTTCGGTTAGACTGGTCGAAGTGCCGGGTTaa        >  2:1194756/1‑149 (MQ=255)                                                                                                                                     aaGCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATTTGCGCGCGGCATTCAGCGCCGACCACGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGACGTGCCGGGTTAac       >  2:487182/1‑149 (MQ=255)                                                                                                                                       gCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacga     <  1:736927/149‑1 (MQ=255)                                                                                                                                       gCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacga     >  2:147559/1‑149 (MQ=255)                                                                                                                                       gCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacga     >  1:149293/1‑149 (MQ=255)                                                                                                                                       gCAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTACCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacga     <  1:1194229/149‑1 (MQ=255)                                                                                                                                        catcGGTCACAAAGCTGAAAAAGATTTGGGCGGAATCGGCCCAGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGTCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacgac    <  2:801391/146‑1 (MQ=255)                                                                                                                                        cAACGGTCACAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAacgac    >  1:948899/1‑149 (MQ=255)                                                                                                                                          aCGGTCACAAAGCTGAAAATGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCTAAGTGCCGGGTTAAcgacga  <  1:1210300/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               GTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA  >  NC_000913_3_bme_pgi/4233651‑4233936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑