Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,756,4970GT54.0% 10.3 / 134.1 87C267F (TGT→TTT) pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base T (24/23);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAA  >  NC_000913_3_bme_pgi/1756353‑1756639
                                                                                                                                                |                                                                                                                                              
ccAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCg                                                                                                                                          >  1:500712/1‑151 (MQ=255)
ccAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATcc                                                                                                                                           <  3:539516/150‑1 (MQ=255)
ccAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGCTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCg                                                                                                                                          <  2:544312/151‑1 (MQ=255)
 ccaaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGt                                                                                                                                         >  1:723812/3‑151 (MQ=255)
 cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCg                                                                                                                                          >  3:39937/1‑150 (MQ=255)
   aaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGc                                                                                                                                       >  2:358221/1‑151 (MQ=255)
       cGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGt                                                                                                                                   >  1:311419/1‑151 (MQ=255)
               aGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGt                                                                                                                           <  1:589181/151‑1 (MQ=255)
                 gAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTa                                                                                                                         <  2:392785/151‑1 (MQ=255)
                    ggCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTATAGTCGTTATCa                                                                                                                      <  2:294178/151‑1 (MQ=255)
                     gCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGGAATCCCGGTAGAAGAAGTGATCTGCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCAc                                                                                                                     >  2:239317/1‑151 (MQ=255)
                        tCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                                                   >  4:112316/1‑150 (MQ=255)
                          aacaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCg                                                                                                                 >  3:543426/1‑150 (MQ=255)
                           acaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGtt                                                                                                                          <  3:361939/140‑1 (MQ=255)
                           acaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGtt                                                                                                                          >  4:361939/1‑140 (MQ=255)
                           acaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGa                                                                                                                >  3:164912/1‑150 (MQ=255)
                           acaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGAc                                                                                                               >  1:128417/1‑151 (MQ=255)
                               cTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAACTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACg                                                                                                                                    <  1:39675/126‑1 (MQ=255)
                               cTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACg                                                                                                                                    >  2:39675/1‑126 (MQ=255)
                                 tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAATTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGAt                                                                                                         <  2:716236/151‑1 (MQ=255)
                                  cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATg                                                                                                        >  2:429968/1‑151 (MQ=255)
                                  cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATg                                                                                                        >  1:635863/1‑151 (MQ=255)
                                   gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGc                                                                                                       <  1:958223/151‑1 (MQ=255)
                                     cgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTg                                                                                                     <  1:81801/151‑1 (MQ=255)
                                       aaaTCCTCGAGGCGTCTGACGGGATCATGGTTGCGCGTGGCGACCTGGGCGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGa                                                                                                   <  2:694033/151‑1 (MQ=255)
                                            cTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCa                                                                                              >  1:72992/1‑151 (MQ=255)
                                             tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAATAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                                                                             <  1:314746/151‑1 (MQ=255)
                                             tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCa                                                                                              >  3:493190/1‑150 (MQ=255)
                                             tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCa                                                                                              >  3:493258/1‑150 (MQ=255)
                                              cGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                                                                             <  4:258680/150‑1 (MQ=255)
                                                aaGCCTCTGACAGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGAt                                                                                          <  2:311508/151‑1 (MQ=255)
                                                    ctctGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaaa                                                                                      >  1:628465/1‑151 (MQ=255)
                                                       tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaaaaa                                                                                    <  4:185527/150‑1 (MQ=255)
                                                        gACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTATAAGAAGTTATCTTCGCCCAGAAAAAGAGGATCGAAAAATTTATCCATGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                                                                                  >  1:139896/1‑151 (MQ=255)
                                                            gCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACg                                                                              <  1:848993/151‑1 (MQ=255)
                                                             catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                                             >  2:617522/1‑151 (MQ=255)
                                                                catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTAt                                                                                                                                             >  1:423430/1‑84 (MQ=255)
                                                                catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTAt                                                                                                                                             <  2:423430/84‑1 (MQ=255)
                                                                  tGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                                         <  3:247263/150‑1 (MQ=255)
                                                                   ggTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                                       <  2:577018/151‑1 (MQ=255)
                                                                         gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                                 <  1:429968/151‑1 (MQ=255)
                                                                         gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                                  >  3:353696/1‑150 (MQ=255)
                                                                          cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                                 <  4:353696/150‑1 (MQ=255)
                                                                            tGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGa                                                               <  3:155945/150‑1 (MQ=255)
                                                                            tGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGa                                                               >  4:431648/1‑150 (MQ=255)
                                                                            gggCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGa                                                               <  4:310614/149‑1 (MQ=255)
                                                                             ggCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGaa                                                              >  3:121339/1‑150 (MQ=255)
                                                                                 aCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCg                                                          <  3:67211/150‑1 (MQ=255)
                                                                                   cTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                                        <  4:424074/150‑1 (MQ=255)
                                                                                     gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAAAAAAAAAAAAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                     <  2:478261/151‑1 (MQ=255)
                                                                                       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgcg                                                                   <  2:559408/135‑1 (MQ=255)
                                                                                       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgcg                                                                   >  1:559408/1‑135 (MQ=255)
                                                                                       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                   >  1:186477/1‑151 (MQ=255)
                                                                                        tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGtt                                                  <  2:723812/151‑1 (MQ=255)
                                                                                        tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                                   <  3:112316/150‑1 (MQ=255)
                                                                                         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                 <  1:320058/151‑1 (MQ=255)
                                                                                           agagATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTACGACCCAGATGCTGGATTCAATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                               >  2:130132/1‑151 (MQ=255)
                                                                                           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                                >  4:39251/1‑150 (MQ=255)
                                                                                           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                               >  1:333939/1‑151 (MQ=255)
                                                                                                tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGCTGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                                           <  4:39937/150‑1 (MQ=255)
                                                                                                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATc                                      >  2:516935/1‑151 (MQ=255)
                                                                                                     gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                                     <  2:914300/151‑1 (MQ=255)
                                                                                                         aagaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                                 >  1:487469/1‑151 (MQ=255)
                                                                                                           gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                                <  4:164912/150‑1 (MQ=255)
                                                                                                           gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                               <  2:72992/151‑1 (MQ=255)
                                                                                                                  tCTTCGCCCAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                        >  2:155119/1‑151 (MQ=255)
                                                                                                                  tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                        >  1:350118/1‑151 (MQ=255)
                                                                                                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                        >  4:327154/1‑150 (MQ=255)
                                                                                                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc                       <  1:617522/151‑1 (MQ=255)
                                                                                                                     tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                      >  3:27306/1‑150 (MQ=255)
                                                                                                                        cccAGAAGATGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTAGACACTACTGACGCAGt                  >  1:293863/1‑151 (MQ=255)
                                                                                                                            gaagaTGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGAt               <  3:215201/150‑1 (MQ=255)
                                                                                                                            gaagaTGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg              <  1:155119/151‑1 (MQ=255)
                                                                                                                            gaagaTGATGATCGAAAAATGGATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATAAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg              <  2:816424/151‑1 (MQ=255)
                                                                                                                             aagaTGATGATCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGc             <  1:358221/151‑1 (MQ=255)
                                                                                                                               gatgatgatCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTg           <  2:311419/151‑1 (MQ=255)
                                                                                                                                    tgatCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGCCGGTACTGACGCAGTGATGCTGTCTgg      >  2:570138/1‑151 (MQ=255)
                                                                                                                                      atCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt     <  3:39251/150‑1 (MQ=255)
                                                                                                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGATGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg    <  2:635863/151‑1 (MQ=255)
                                                                                                                                       tCGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg    >  3:387366/1‑150 (MQ=255)
                                                                                                                                        cGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg    >  1:387187/1‑149 (MQ=255)
                                                                                                                                        cGAAAAATTTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg    <  2:387187/149‑1 (MQ=255)
                                                                                                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAt                                                                                                  <  2:634121/55‑1 (MQ=255)
                                                                                                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAt                                                                                                  >  1:634121/1‑55 (MQ=255)
                                                                                                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATc                                                                                         >  2:678381/1‑64 (MQ=255)
                                                                                                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATc                                                                                         <  1:678381/64‑1 (MQ=255)
                                                                                                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa  >  4:43979/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAA  >  NC_000913_3_bme_pgi/1756353‑1756639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑