Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,262,2510GC29.4% 45.7 / 29.8 34G194G (GGG→GGC) dusAtRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base G (10/14);  new base C (6/4);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATAT  >  NC_000913_3_bme_pgi/4262102‑4262394
                                                                                                                                                     |                                                                                                                                               
gtggggTCGCTTCCGGTGACGGCGAAAACGCGAATTGGCAACGACGAGCAGGACAGATACGCAGTACCCTGCGAATCCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCAAGCACGTAAAGCCCGGCTTTCgggg                                                                                                                                                 >  4:205819/1‑150 (MQ=255)
 tggtgTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGt                                                                                                                                                >  4:194482/1‑150 (MQ=255)
  ggtgTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGtt                                                                                                                                               <  1:344974/150‑1 (MQ=255)
             cggtgacggtgaAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaag                                                                                                                                    <  4:27223/150‑1 (MQ=255)
                     gtgaAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGt                                                                                                                            <  2:100143/150‑1 (MQ=255)
                            cgcgTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAAAACCGTTACCGGCAAAGGCTAGTGTGTGTTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATcc                                                                                                                     <  1:403795/150‑1 (MQ=255)
                                        tCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTATGATATGTTGATCATCAACGCACGTAAAGCCTGGCTTTAGGGGCTAATCTCGCAAGAAAACCGAGAAATCCAACCGCCCGAtt                                                                                                         >  2:399952/1‑150 (MQ=255)
                                             gacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaagccaggc                                                                                                                              >  2:470223/1‑118 (MQ=255)
                                             gacCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaagccaggc                                                                                                                              <  1:470223/124‑7 (MQ=255)
                                               ccAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaa                                                                                                                                     >  1:291725/1‑115 (MQ=255)
                                               ccAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaa                                                                                                                                     <  2:291725/115‑1 (MQ=255)
                                                cAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCccgccg                                                                                                                <  1:194108/135‑1 (MQ=255)
                                                cAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCccgccg                                                                                                                >  2:194108/1‑135 (MQ=255)
                                                  ggACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGAGAGTGTGAGATGTTCATCATCCACGCACGTAACGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAATTCCCGCCGCTCGATTATCCGCgtgt                                                                                               <  1:399952/150‑1 (MQ=255)
                                                                     tGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGAc                                                                            <  3:194482/150‑1 (MQ=255)
                                                                          tttCATCAATACCGTTTCCGGCAAAGGCTATTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTcc                                                                       <  1:284050/150‑1 (MQ=255)
                                                                                                      gAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggtg                                             >  1:442316/1‑148 (MQ=255)
                                                                                                      gAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggtg                                             <  2:442316/148‑1 (MQ=255)
                                                                                                      gAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaa                                                                                                                                     >  4:239153/1‑60 (MQ=38)
                                                                                                      gAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaaa                                                                                                                                     <  3:239153/60‑1 (MQ=38)
                                                                                                            gagaTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt                                              <  1:79917/141‑1 (MQ=255)
                                                                                                            gagaTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt                                              >  2:79917/1‑141 (MQ=255)
                                                                                                                        atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATc                                                                  <  2:115317/109‑1 (MQ=255)
                                                                                                                        atcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATc                                                                  >  1:115317/1‑109 (MQ=255)
                                                                                                                          ccACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGc                       >  1:194777/1‑150 (MQ=255)
                                                                                                                            acgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCgt                                                                                                 >  2:262086/1‑74 (MQ=255)
                                                                                                                            acgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCgt                                                                                                 <  1:262086/74‑1 (MQ=255)
                                                                                                                                 ccgaaaGCCAGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGca                >  4:248549/4‑150 (MQ=255)
                                                                                                                                  gTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGcac               <  2:194777/150‑1 (MQ=255)
                                                                                                                                    aaaGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt                                              >  4:130600/1‑117 (MQ=255)
                                                                                                                                    aaaGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt                                              <  3:130600/117‑1 (MQ=255)
                                                                                                                                           ttcTTTCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGCCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAAc      >  1:9866/3‑150 (MQ=255)
                                                                                                                                              tttCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga                          <  3:2720/127‑1 (MQ=255)
                                                                                                                                              tttCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga                          >  4:2720/1‑127 (MQ=255)
                                                                                                                                               ttCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACatat  >  4:44094/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATAT  >  NC_000913_3_bme_pgi/4262102‑4262394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑