Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 3,440,086 C→A G315V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,440,0860CA100.0% 228.5 / NA 64G315V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/32);  total (32/32)

CCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCA  >  NC_000913_3_bme_pgi/3439945‑3440232
                                                                                                                                             |                                                                                                                                                  
ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGAc                                                                                                                                            >  1:416408/1‑150 (MQ=255)
    gCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTc                                                                                                                                        <  4:153039/150‑1 (MQ=255)
         ggCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACgg                                                                                                                                   <  2:52123/150‑1 (MQ=255)
           ctgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGa                                                                                                                                 >  1:547356/1‑150 (MQ=255)
            tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGaa                                                                                                                                <  2:308503/150‑1 (MQ=255)
                  tGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGc                                                                                                                          <  3:126194/150‑1 (MQ=255)
                   gCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCa                                                                                                                         <  3:168704/150‑1 (MQ=255)
                   gCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCAACGGGAAGCCAGCa                                                                                                                         >  3:540681/1‑150 (MQ=255)
                       ttCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGc                                                                                                                              >  2:973424/1‑141 (MQ=255)
                       ttCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGc                                                                                                                              <  1:973424/141‑1 (MQ=255)
                            ttGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTaa                                                                                                                >  4:558701/1‑150 (MQ=255)
                               acgacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct                                                                                                             >  4:252608/1‑150 (MQ=255)
                               acgacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct                                                                                                             >  1:925489/1‑150 (MQ=255)
                                 gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                           >  3:53142/1‑150 (MQ=255)
                                  acCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAg                                                                                                          >  3:129566/1‑150 (MQ=255)
                                       tctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAaga                                                                                                     >  4:578074/1‑150 (MQ=255)
                                             gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttt                                                                                               >  1:325872/1‑150 (MQ=255)
                                                  cgcATGACCTTATCCTTCTCAGTAAAACCTTAAACTGTGATCCGGTTACTCCTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGCGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTAcc                                                                                          >  1:735146/1‑150 (MQ=255)
                                                         ccTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCaga                                                                                                                                               <  1:718116/90‑1 (MQ=255)
                                                         ccTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCaga                                                                                                                                               >  2:718116/1‑90 (MQ=255)
                                                          cTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                  >  2:462867/1‑150 (MQ=255)
                                                           ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                                 <  4:607025/150‑1 (MQ=255)
                                                               ccTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                             >  4:631491/1‑150 (MQ=255)
                                                               ccTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                             <  1:222658/150‑1 (MQ=255)
                                                                   ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                         >  1:705113/1‑150 (MQ=255)
                                                                   ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                         >  1:354801/1‑150 (MQ=255)
                                                                    tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                        <  2:416408/150‑1 (MQ=255)
                                                                    ccAGTAAAGCCTTCACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGGTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCGTTGATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                        <  2:735146/149‑1 (MQ=255)
                                                                       gTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATACTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTgaggag                                                                     >  1:950201/1‑150 (MQ=255)
                                                                         aaaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                   <  2:925489/150‑1 (MQ=255)
                                                                             ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                               <  2:325872/150‑1 (MQ=255)
                                                                              cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                                              >  4:495127/1‑150 (MQ=255)
                                                                               ttAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCt                                                             <  1:290133/150‑1 (MQ=255)
                                                                                 aaCCTGTGATCCGGTTACTCGTCAGCGATACTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTGAGGAGCTCAACCTCg                                                           <  2:950201/150‑1 (MQ=255)
                                                                                  aCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                          <  4:129566/150‑1 (MQ=255)
                                                                                     tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttt                                                                                               >  2:758092/1‑110 (MQ=255)
                                                                                     tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttt                                                                                               <  1:758092/110‑1 (MQ=255)
                                                                                        gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                           >  2:81506/1‑143 (MQ=255)
                                                                                        gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                           <  1:81506/143‑1 (MQ=255)
                                                                                        gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCt                                                    <  1:346212/150‑1 (MQ=255)
                                                                                               ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtctc                                                                                                            <  3:552797/87‑1 (MQ=255)
                                                                                               ttACTCGGCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtctc                                                                                                            >  4:552797/1‑87 (MQ=255)
                                                                                                  cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                          <  1:73824/150‑1 (MQ=255)
                                                                                                   tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCa                                         <  4:53142/150‑1 (MQ=255)
                                                                                                    cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                                   <  4:448685/139‑1 (MQ=255)
                                                                                                    cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                                   >  3:448685/1‑139 (MQ=255)
                                                                                                     gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCaga                                                                                                                                               >  1:704290/1‑46 (MQ=255)
                                                                                                     gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCaga                                                                                                                                               <  2:704290/46‑1 (MQ=255)
                                                                                                     gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                       <  3:135791/150‑1 (MQ=255)
                                                                                                      tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                       <  3:588633/117‑1 (MQ=255)
                                                                                                      tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                       >  4:588633/1‑117 (MQ=255)
                                                                                                            aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCaa                                                                                        <  1:572224/94‑1 (MQ=255)
                                                                                                            aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCaa                                                                                        >  2:572224/1‑94 (MQ=255)
                                                                                                            aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                                <  3:578074/150‑1 (MQ=255)
                                                                                                                    cGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                  >  2:368654/1‑92 (MQ=255)
                                                                                                                    cGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                  <  1:368654/92‑1 (MQ=255)
                                                                                                                      gTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGCACCAGATCACCGATATAGTGGATAGCtt                      >  3:580851/1‑150 (MQ=255)
                                                                                                                         gCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCGGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                   >  2:212011/1‑150 (MQ=255)
                                                                                                                         gCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                   <  2:354801/150‑1 (MQ=255)
                                                                                                                         gCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                   >  2:578571/1‑150 (MQ=255)
                                                                                                                              ttttCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa              <  3:252608/150‑1 (MQ=255)
                                                                                                                                ttCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGTACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg            <  3:558701/150‑1 (MQ=255)
                                                                                                                                    aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg        >  3:310362/1‑150 (MQ=255)
                                                                                                                                          aTGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa  >  1:144464/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCA  >  NC_000913_3_bme_pgi/3439945‑3440232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: