New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 164559 =42 (1.060)6 (0.160) 5/268 4.8 14.1% intergenic (+25/‑171) hrpB/mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
?NC_000913_3_bme_pgi = 3959482 34 (0.920)intergenic (+37/+50) ilvC/ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAT  >  NC_000913_3_bme_pgi/3959335‑3959626
                                                                                                                                                   |                                                                                                                                                
gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                                 <  1:125327/149‑1 (MQ=255)
             aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcggg                                                                                                                                    <  1:68053/149‑6 (MQ=255)
                   aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                                              <  2:416899/149‑1 (MQ=255)
                        gCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                         <  2:159304/149‑1 (MQ=255)
                           aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCtt                                                                                                                      >  2:3335/1‑149 (MQ=255)
                                cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGAAAAGTATGAAACTTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                 <  1:478522/149‑1 (MQ=255)
                                  gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAGATCACTCAAGGCCTTCTCCTgg                                                                                                               <  2:6532/149‑1 (MQ=255)
                                   ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                              <  1:3335/149‑1 (MQ=255)
                                        cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAgg                                                                                                         <  1:83732/149‑1 (MQ=255)
                                         gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                                        <  2:542834/149‑1 (MQ=255)
                                                   gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                              <  2:577674/149‑1 (MQ=255)
                                                        gaaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                         <  1:73751/148‑1 (MQ=255)
                                                         aaGAACTTGCGCGGCTATAGGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACACGCCGGCGATGAAGATGCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                        <  2:461709/149‑1 (MQ=255)
                                                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                        <  2:653181/149‑1 (MQ=255)
                                                            aaaCTGCGCGGCTATATGACAGATATGAAGCGGATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                     <  2:110579/149‑1 (MQ=255)
                                                               cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTCGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCAGAATGCTTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                                                  <  1:512970/149‑1 (MQ=37)
                                                                 gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCTACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                <  1:526697/149‑1 (MQ=255)
                                                                         atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgt                                                                                               >  1:190151/1‑121 (MQ=37)
                                                                         atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgt                                                                                               <  2:190151/126‑6 (MQ=37)
                                                                            tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                     >  2:559212/1‑149 (MQ=255)
                                                                                       aaaCGTATTGCTGTTGCGGGTTAAGTGAGAGCTAGCGCCCCCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTTATGATGTGATATCCGaa                                                          >  1:341330/1‑149 (MQ=38)
                                                                                                          gTTAAGTGCGCGCTGATGCCATCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGAGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcag                                       >  1:41256/1‑147 (MQ=255)
                                                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGCAAAAACTCAAGGGCTTCTCCTGGAGAAGGACTTGCTATTGGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACCGGGTGTGCAGCGGGCCGGTCGGccca                              >  2:115151/1‑146 (MQ=38)
                                                                                                                         aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                        >  2:463462/1‑149 (MQ=255)
                                                                                                                               tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                  >  1:565845/1‑149 (MQ=255)
                                                                                                                                 aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                <  1:559212/149‑1 (MQ=255)
                                                                                                                                      gccccTCTCCCACGGGGAGAGGCAGAAAACACTCCAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa           <  1:675829/147‑1 (MQ=255)
                                                                                                                                         ccTCTCCCACAGGGCGAGGGAGACAACACTCAAGGCCTTCTCCTGGAGAAGGACTTGCTAAGAGTTGCGGTACAGCACCTTAATGATGTGAGATCCGAACTCGGCGCCCAGCGGGCCGGTCGGCGCGACTACGGGACCAGCGCAAAccc        >  2:430960/1‑148 (MQ=255)
                                                                                                                                         ccTCGCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa        <  2:188147/149‑1 (MQ=255)
                                                                                                                                          ctctCCGACAGGGAGAGCGAGAAAACACTCAAGTCCTTCTCCTAGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc       <  2:452189/149‑1 (MQ=255)
                                                                                                                                          ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc       >  1:95393/1‑149 (MQ=255)
                                                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt      >  2:357271/1‑149 (MQ=255)
                                                                                                                                            ctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACCGCACCTTAATGAGGTGATATCCGACCGGGGTGTGCAGCGGGCCGGTCGGCGCCAGTACAGTAACAGAGGAAACCACtt     >  2:90989/1‑149 (MQ=255)
                                                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt  >  1:180698/1‑149 (MQ=255)
                                                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                    <  2:595998/49‑1 (MQ=255)
                                                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                    >  1:595998/1‑49 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAT  >  NC_000913_3_bme_pgi/3959335‑3959626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.