Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 1,196,093 C→T P324L (CCT→CTT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi1,196,0930CT100.0% 84.7 / NA 26P324L (CCT→CTT) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

CGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGC  >  NC_000913_3_bme_pgi/1195947‑1196237
                                                                                                                                                  |                                                                                                                                                
cGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTg                                                                                                                                                <  2:307070/149‑1 (MQ=255)
       aaaGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCaaa                                                                                                                                         <  1:743155/149‑1 (MQ=255)
                aTTGCGGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAAGATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGa                                                                                                                                <  2:600908/149‑1 (MQ=255)
                      gATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATGTGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATg                                                                                                                          >  2:554884/1‑149 (MQ=255)
                           aTTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTg                                                                                                                                                >  1:543499/1‑122 (MQ=255)
                           aTTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTg                                                                                                                                                <  2:543499/122‑1 (MQ=255)
                            ttCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCt                                                                                                                    >  2:471066/1‑149 (MQ=255)
                                        aTCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCACACATCGGTGACGAATGCGCCCTGTTTGAGGacac                                                                                                        >  1:692941/1‑149 (MQ=255)
                                             gctgcGTCCGGCTGAAGATGAGGTTATCGCCTGGATGAACCTGAAGGGTCATTGTCTTGCTGACGCCCTGGCAGGGCCGGTTGGTGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACg                                                                                                   <  2:532996/149‑1 (MQ=255)
                                               tgcGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGAAGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGACTGAGCCCTGTTTGAAGACCCCCACGGt                                                                                                 >  1:523300/1‑149 (MQ=255)
                                                    ccGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTgc                                                                                            <  1:527981/149‑1 (MQ=255)
                                                    ccGGCTGAATATGATGCTATCGCCGGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTgc                                                                                            <  1:590688/149‑1 (MQ=255)
                                                      ggCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGt                                                                                                                                  <  1:638610/109‑1 (MQ=255)
                                                      ggCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGt                                                                                                                                  >  2:638610/1‑109 (MQ=255)
                                                        cTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCAGCCACGGTACTccgccg                                                                                        >  2:157862/1‑149 (MQ=255)
                                                          gAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGaa                                                                                      <  2:298022/149‑1 (MQ=255)
                                                                            tGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAgg                                                                        >  2:656982/1‑145 (MQ=255)
                                                                            tGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAgg                                                                        <  1:656982/145‑1 (MQ=255)
                                                                                   aCCTGAACGGTGACTACATTTCTGACGCCCTGGCAGGGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAATGTAAAt                                                             >  2:608081/1‑149 (MQ=255)
                                                                                     cTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATcc                                                           >  1:3221/1‑149 (MQ=255)
                                                                                                                       cgcAGGTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCGGAGATGctgctg                         >  1:286471/1‑149 (MQ=255)
                                                                                                                            gTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGaaa                                                                                     >  2:282313/1‑84 (MQ=255)
                                                                                                                            gTTGGCGGTATCGGTATCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGaaa                                                                                     <  1:282313/84‑1 (MQ=255)
                                                                                                                                       cggtatcgCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGa         <  1:583836/149‑1 (MQ=255)
                                                                                                                                             cgCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCACGTTGAGATGATGCTGCGCCACATGGGTTGGACCGAAg   <  1:554884/149‑1 (MQ=255)
                                                                                                                                              gCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGc  >  2:416373/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGC  >  NC_000913_3_bme_pgi/1195947‑1196237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: