Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,184,810	G→T	G1189V (GGT→GTT)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,184,810	0	G	T	100.0%	219.9 / NA	68	G1189V (GGT→GTT)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/39);  total (29/39)

AACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCA  >  NC_000913_3_bme_pgi/4184663‑4184954                                                                                                                                                    |                                                                                                                                                 aaCTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTg                                                                                                                                                <  2:1206003/150‑1 (MQ=255)        cgAATTCAGCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAaaga                                                                                                                                         <  2:1359996/150‑1 (MQ=255)            ttCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCa                                                                                                                                     <  2:891424/150‑1 (MQ=255)                 ccAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAAt                                                                                                                                <  2:1147719/150‑1 (MQ=255)                   aGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGAAGTTCGACGTTACGAAAGAAGCAGAAATTa                                                                                                                              >  1:296981/1‑150 (MQ=255)                     cgtgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTaaa                                                                                                                            <  1:1113494/150‑1 (MQ=255)                                   gggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACt                                                                                                              >  2:1161229/1‑150 (MQ=255)                                    ggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACtt                                                                                                             >  1:1125467/1‑150 (MQ=255)                                     gcgcTGACGTTCGTCAGAAAGTTGACCTGAGTACGTTCCGTGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTg                                                                                                            <  2:1336037/150‑1 (MQ=255)                                      cgcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTgg                                                                                                           <  1:1144740/150‑1 (MQ=255)                                            cGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCt                                                                                                     <  2:870986/150‑1 (MQ=255)                                                 gTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAACTTATTTAGCTGCTGAAACTTGGCGACCTGCCGa                                                                                                <  2:788637/150‑1 (MQ=255)                                                  tCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAGGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGAc                                                                                               >  2:753121/1‑150 (MQ=255)                                                  tCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAACGGCATGCCAATCGCAACGCCGGTGTTCGACGTGGCGAAAGAAGCAGAACTTAAAGAGCTGCTGAAACTTGCGGACTGGCCGAc                                                                                               >  2:502629/1‑150 (MQ=255)                                                       aaGTTGACATGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCg                                                                                          <  2:468931/150‑1 (MQ=255)                                                         gTTGACCTGAGTACCTTCAGCTATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGt                                                                                        <  2:1303581/150‑1 (MQ=255)                                                          ttGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                       >  1:36554/1‑150 (MQ=255)                                                             aCCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATACCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGa                                                                                    >  1:742216/1‑150 (MQ=255)                                                                       cTTTAGCGATGAATAAGTTAGGCCTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTTCCGACTTCCGGTCAGATCCGCCTGTa                                                                          <  1:307254/150‑1 (MQ=255)                                                                       cTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                                          <  2:1125467/150‑1 (MQ=255)                                                                           aGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGCATGCCAATCCCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGAt                                                                      >  2:1313120/1‑150 (MQ=255)                                                                               aTGAAGAAGTTATGCGTCTGGCTGAAAACCTACCCAAAGGTATGCCAATCGCAACGCCTGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTc                                                                  <  1:426239/150‑1 (MQ=255)                                                                                agaagaaGTTATGCGTCTGGCGGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCg                                                                 <  1:231271/149‑1 (MQ=255)                                                                                 gaagaaGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGc                                                                >  2:1161701/1‑150 (MQ=255)                                                                                 gaagaaGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGc                                                                >  1:1290957/1‑150 (MQ=255)                                                                                    gaagTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGCCCTGCCGACTTCCGGGCAGATCAGCCTGTGCGATGGTCACACt                                                             >  1:479691/1‑150 (MQ=255)                                                                                       gTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTACGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGt                                                          <  1:753121/150‑1 (MQ=255)                                                                                        ttATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                                         <  1:1052439/150‑1 (MQ=255)                                                                                          agtggTCTGGCTGAAAACGTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGaa                                                       <  2:374933/146‑1 (MQ=255)                                                                                          aTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGaa                                                       <  2:590075/150‑1 (MQ=255)                                                                                            gCGTCTGGCTGAAAACCTTGCCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGGAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACa                                                     <  1:22662/150‑1 (MQ=255)                                                                                            gCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTTATTATCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACa                                                     <  1:545823/150‑1 (MQ=255)                                                                                            gCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACa                                                     <  1:878985/150‑1 (MQ=255)                                                                                              gTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGt                                                   >  2:1304126/1‑150 (MQ=255)                                                                                                cTGGCTGAAAACCTGCGCAACGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTc                                                 <  1:715/150‑1 (MQ=255)                                                                                                   gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                              <  2:450583/150‑1 (MQ=255)                                                                                                   gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                              >  2:137862/1‑150 (MQ=255)                                                                                                   gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                              <  1:137862/150‑1 (MQ=255)                                                                                                     tGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCt                                                                             >  2:241481/1‑117 (MQ=255)                                                                                                     tGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCt                                                                             <  1:241481/117‑1 (MQ=255)                                                                                                     tGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCg                                            >  1:975421/1‑150 (MQ=255)                                                                                                            cTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTa                                     >  1:1276507/1‑150 (MQ=255)                                                                                                            cTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTa                                     >  2:603609/1‑150 (MQ=255)                                                                                                             tGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTac                                    >  2:664281/1‑149 (MQ=255)                                                                                                             tGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTaa                                    <  2:36554/150‑1 (MQ=255)                                                                                                             tGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTaa                                    <  1:1313120/150‑1 (MQ=255)                                                                                                                 cAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATACGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAACGTCCGGTAACCGt                                >  2:838814/1‑150 (MQ=255)                                                                                                                  aaaGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGtt                               >  1:407392/1‑150 (MQ=255)                                                                                                                    aGGGATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTgg                             <  1:354723/150‑1 (MQ=255)                                                                                                                       tATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGc                                             >  1:1133118/1‑131 (MQ=255)                                                                                                                       tATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGc                                             <  2:1133118/131‑1 (MQ=255)                                                                                                                        ataccAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtac                         <  2:742216/147‑1 (MQ=255)                                                                                                                        aTGTCAATCGCAACGCCGGTGTTCGACCTAAGGAAGTCAGCAGAAATTTAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTCACCAGTTTCAGCGTCCGGTAACCGTGGGttgc                         >  2:303414/1‑148 (MQ=255)                                                                                                                          gCCAATCGCACCGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacat                       <  1:555290/150‑1 (MQ=255)                                                                                                                          gCCAATCGCAACGCCGGTGTTGGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGCGCATATGTTGGGCGTCCGGTAACCGTTGGTtacat                       <  2:1004279/150‑1 (MQ=255)                                                                                                                          gCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGATGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacat                       <  2:1063067/150‑1 (MQ=255)                                                                                                                            cAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTCACCGTTGGTtacatgt                     >  1:1277849/1‑150 (MQ=255)                                                                                                                            cAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgt                     <  2:1290957/150‑1 (MQ=255)                                                                                                                               tCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgtaca                  >  2:534282/1‑150 (MQ=255)                                                                                                                                 gCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgtacatg                <  1:603609/150‑1 (MQ=255)                                                                                                                                  cAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGACACTTGGCGACCTGCCAACTTCCGGTCAGATACGCCTGTACGATGGTCGCACAGGTGAACAGATAGAGCGTCCGCTATCCGTTGGTTACATGTCCATGc               >  2:250639/1‑150 (MQ=255)                                                                                                                                   aaCGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGc               <  2:100774/149‑1 (MQ=255)                                                                                                                                   aaCGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGc               >  1:100774/1‑149 (MQ=255)                                                                                                                                        cGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAAc         >  1:567033/1‑150 (MQ=255)                                                                                                                                             ttCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAac    <  1:1304126/150‑1 (MQ=255)                                                                                                                                             ttCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAac    <  1:1161229/150‑1 (MQ=255)                                                                                                                                             ttCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAac    >  1:825951/1‑150 (MQ=255)                                                                                                                                               cGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTACGGTCACATGCGCCTGTACGATCGGCGCCCTGATGGCAACGCTGAGCGTGCGGTAACCTTGGGTGACAGTTACATGCTGAAACTGAacca  >  2:1169851/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCA  >  NC_000913_3_bme_pgi/4184663‑4184954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑