Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_bme_pgi | 380,013 | 0 | G | . | 27.8% | 33.9 / 14.2 | 18 | intergenic (‑132/+56) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
Reads supporting (aligned to +/- strand): ref base G (13/0); new base . (4/1); total (17/1) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTG > NC_000913_3_bme_pgi/379866‑380148 | ccGGAGTACAGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg < 2:261942/150‑1 (MQ=255) ggAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTTGTCGGAAAACAAAGAGGAAAggggg > 1:385912/1‑150 (MQ=255) aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg > 2:688968/1‑139 (MQ=255) aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg < 1:688968/139‑1 (MQ=255) catcTGCATGCAATATACTATAGGGGGGTTTTCTCTATGTCAATGCCTACCCCCCTATAGTATATACCACTGGAGAATATAATTTTTCCGGTGAATGTGGGCGGAAAACATAGAGGAAAGGGGGGGGGGCTAATCGGCGGGGAAGgccgc > 2:438556/1‑150 (MQ=255) tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg > 1:700558/1‑150 (MQ=255) atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGATAAGCGGCAGGGAAGGCCGCCCCGGAt > 2:762072/1‑150 (MQ=255) gcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg > 1:782672/1‑150 (MQ=255) taatataTGGGGGTATTGAATATGTCCATGCATACCCCCGAATAGTATATAACACTGGAGAATAATAATTATCCGCGGAATGTGGGCGGAAAACAAACAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg < 2:867788/147‑1 (MQ=255) tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGCCAGGGAAGCCCGCCCCGGATACCGGGCGGCAGAAGGAATCGGAATTTCCAgg > 2:889030/1‑150 (MQ=255) ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCGTATAGGCAGGGAAGGGCGCCCCGGATAGGGGGGGGCCGAAGGAATGAGAATTTGCAGGTCAGACggggg > 1:50089/1‑148 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGAAGAAGGAATAAGAATTTCCGGGTCAGACGGCCTCCaat > 1:305892/1‑149 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 1:487480/1‑150 (MQ=255) tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCCGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCCGGGCAGCCGGGCAGCAAg > 1:906965/1‑150 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGTGATAAACGGAAGTGAAGGCCTCCCCGGATAGAGGGCGGAAGAAGGAATAAGAATTTCAAGGACAGACGGGCTGAAAGTTgaa > 2:599618/1‑148 (MQ=255) cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCTGGGAAGGCCGCCCCGGATAGCGGGCGGCAGCAGGAATCCGAATTTCCAGGACAGACGGGCTGCAAGTTGCAGCCCGGTaa > 2:775262/1‑150 (MQ=255) aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGAAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAATTTGCAGACCGTTAAAatcat > 2:139168/1‑150 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCTGGGGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg > 1:214312/1‑150 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGCCCGTTAAAATCATCGGTTGGGGTGTCGTa > 1:407446/1‑150 (MQ=255) ggTAGGAAAACAAAGAGGAAAGAGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGCTAGCGGGCGGCAGAAGCAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCTTTAAAATCATCGGTTGGGGTGTCGTACCACACt > 2:778288/1‑150 (MQ=255) cGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGATTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTa > 1:536351/1‑150 (MQ=255) aaaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTg > 1:728873/1‑150 (MQ=255) | CCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTG > NC_000913_3_bme_pgi/379866‑380148 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |