| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 2,173,361 | 0 | C | . | 95.0% | 168.9 / 0.4 | 40 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (20/18); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_bme_pgi | 2,173,362 | 0 | C | . | 97.4% | 167.0 / ‑1.3 | 40 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (20/18); minor base T (0/1); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT > NC_000913_3_bme_pgi/2173214‑2173502 || ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTCAGTGGGATAAAAATCAGGCTTGCC‑GATaccacc > 1:95427/1‑149 (MQ=255) aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCcagca < 1:132978/149‑1 (MQ=255) aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCcagca < 1:330766/149‑1 (MQ=255) cATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCaa < 1:304534/149‑1 (MQ=255) aTCGCCACGAAGAAGCTGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 1:69073/149‑1 (MQ=255) cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc < 2:369178/149‑1 (MQ=255) ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAATAAAATGGTGAGTGGGATAAAAATCCGACTTGCC‑GATACCA‑‑CCGCCGTAAGTCCCAGCAGCAACGTCGGAGCaaccacc > 2:505448/1‑144 (MQ=255) aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg < 1:454538/149‑1 (MQ=255) gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGa > 1:596783/1‑149 (MQ=255) aagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 2:180239/149‑1 (MQ=255) ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAACATCCGGCATGCC‑GGTAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc < 2:722568/149‑1 (MQ=255) ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc < 1:370406/149‑1 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc > 2:162126/1‑149 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCGGGAAGTCCt > 1:226852/1‑149 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt > 1:115260/1‑149 (MQ=255) tggtggCAAGATCGCCAAACGGGAGCACCTGATTACCCGGCACACAAACAGCAATTAAAGTTGTGAGTGGGATAAAAATCAGTCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGa < 2:422911/149‑1 (MQ=255) aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCCATTAAAATGGTAAGTGGGAGAAAAATCAGGCTGGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGaa < 2:618869/149‑1 (MQ=255) tCGCCAAACGGCAGACACTGATTACCCGTCACACAACCAGCAGTTTTTATGGTACCCGGGTTAATGA‑CTTCCTTTCCTGGTTCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAACtt > 2:389764/1‑149 (MQ=255) ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc > 2:674598/1‑149 (MQ=255) aaaCGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCt > 2:310506/1‑149 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg < 2:831637/120‑1 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg > 1:831637/1‑120 (MQ=255) cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt < 2:168597/98‑1 (MQ=255) cTGATTAACCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt > 1:168597/1‑98 (MQ=255) aTTAACCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg > 2:637207/1‑149 (MQ=255) accaaCACCAATTACAACGGTGTGTGCGACAAAAACCAGGCTTGCC‑GCTAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGATCTCCTGACCGCTGAACGTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa < 1:334810/146‑1 (MQ=255) cAGCAATTGAAAAGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAGTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc < 2:259578/149‑1 (MQ=255) aGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg > 2:780424/1‑149 (MQ=255) aGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTACC‑GATACCA‑‑CCGCCGTATGTACCAGAAGTATCGTTTTATTAAGTCCAAACAGGAAATACCGACAGCCGAACTTCGCCTGTAAACCACTACGAGCCTGCTTACCGATggcag > 2:555813/1‑146 (MQ=255) aGCAATTAAAATGGTGAGAGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCACAGCAGCAACACCGGATCAAGGCCAATCAGGAACTCCTAACAGCAGAACTTCGCCTGTAAAANACTNCGAGCCTGCTTAGCGATGGGCg > 1:781352/1‑149 (MQ=255) cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt > 1:821089/1‑149 (MQ=255) cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTACCGATAGGCGtt > 2:668175/1‑149 (MQ=255) tGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc < 1:780424/149‑1 (MQ=255) aGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc > 1:558009/1‑88 (MQ=255) aGTGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc < 2:558009/88‑1 (MQ=255) tGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg > 2:319860/1‑149 (MQ=255) tGGGATAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTCCTTAGCGATGGGCCGTAAACCACCCCTGCTgg > 2:113611/1‑149 (MQ=255) aTAAAAATCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt > 2:461425/1‑149 (MQ=255) aaaaaTCAGGCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 1:672313/149‑1 (MQ=255) ggCTTGCC‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt < 1:162126/149‑1 (MQ=255) cc‑GATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt < 2:475094/149‑1 (MQ=255) || GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT > NC_000913_3_bme_pgi/2173214‑2173502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |