| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 3,560,455 | 1 | . | G | 96.8% | 92.9 / ‑3.8 | 31 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (9/21); total (9/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC > NC_000913_3_bme_pgi/3560307‑3560589 | gacGATATGGATAAAGTGGTGCGAGCCATTGGGGTTTTGCGCAGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGc < 2:316945/147‑1 (MQ=255) gaaatCCATAACCAGCGTCCAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGcgca < 2:580381/146‑4 (MQ=255) aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGttt < 1:600203/149‑1 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGACCTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGc < 2:559439/149‑1 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGAGGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCa < 1:349608/149‑1 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg > 1:701188/1‑149 (MQ=255) cATTGGGGATTTGCTCCGCCAGTTTGCGGGCGATGCGCTCGTTTCCTTCGGCCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCT‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc < 2:664050/149‑1 (MQ=255) ggggTTTGGTCCGGCGCTTTGGGGGCGATGCGCTTTTTTTGTTCGGTCTGGCTGGCCTTTCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGTTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 2:141509/149‑1 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt > 1:384207/1‑149 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAg < 2:380470/126‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAg > 1:380470/1‑126 (MQ=255) ccGCCACCTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 1:728240/149‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 1:678596/149‑1 (MQ=255) cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:718303/149‑1 (MQ=255) tGCGGGCGATGGGCTCTTTTTCATCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc < 2:193495/149‑1 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGc > 1:219433/1‑149 (MQ=255) tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga > 1:713662/1‑149 (MQ=255) tttCTTCGGTCTGGGTTGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa < 1:612662/149‑1 (MQ=255) tttCTTCGGGCTGGTGGGCCTTGCGATTGTGCCACGGGATGTTAACCGAACTGAAAGGCAGCGCCGCACCGCC‑ATGATGCCGCAGCATCAGGTTTTCCGCCGCCAGCTCATCGAGGTCGCGCCGAATATTCTGCGGGCTGACGGAGaaa < 2:526046/149‑1 (MQ=255) tgttgggTCTGGGTGGCCTGGCGATCGGGGCACGGGGTGTTGGCCGAGCTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGGTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg < 2:335783/144‑1 (MQ=255) cttcGGGCTGGGTGGCTTTGCGATCGTGGCACGGCGTGTGAACCGAACTGGAAGGCAGGGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc < 2:644854/149‑1 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa < 2:701188/149‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc > 1:425098/1‑149 (MQ=255) cTGGGGGGCCTTGCGAGCGAGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGCATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa < 1:398884/149‑1 (MQ=255) gggCGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTATCTTCAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGc < 1:825570/149‑1 (MQ=255) cggtCGGGCCTCGGGGTGGTAACCGGACTGGAGGTCAGCGCCGCACCGCC‑ATGATGGCGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt < 2:671897/146‑1 (MQ=255) cGTGTTAACCGAATGGGAAGGCAGCGTCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:318921/149‑1 (MQ=255) ggtAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGAGGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATATGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 2:388620/147‑1 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt > 1:404938/1‑149 (MQ=255) tAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa > 2:464933/1‑86 (MQ=255) tAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:464933/86‑1 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTTAACCa > 2:708325/1‑149 (MQ=255) gccGCCAATGAGGGG‑CAGGGTCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAc < 2:292055/148‑1 (MQ=255) | GCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCC‑ATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC > NC_000913_3_bme_pgi/3560307‑3560589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |