| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,296,382 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,296,381 | 1 | . | C | 87.5% | 96.4 / 11.3 | 40 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base C (17/18); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_bme_pgi | 4,296,381 | 2 | . | G | 87.5% | 98.2 / 11.4 | 40 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (17/18); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCT > NC_000913_3_bme_pgi/4296297‑4296519 || gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg > 2:852863/1‑149 (MQ=12) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTATAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt > 1:885910/1‑149 (MQ=255) cGCTTAACGCGTCTTATCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt < 1:90534/149‑1 (MQ=255) gCTTAACCCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTCG‑CGCGATTTTGTAGGTCGGATAAGGCGTT‑‑TCCG‑‑C‑C‑GCATCCGACATCAATGCCTCATGCGCAGCTTCACGCGTTTCATCATTCCTATCTTAACCTTTg > 2:711869/1‑149 (MQ=11) ccTTAACGTCTCTTAACAGGCCTAAGCCAAACACCGCAATAGCCTGATTTAG‑CGTGATTTTATAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:294246/148‑1 (MQ=11) cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCGTTgg > 2:108936/1‑149 (MQ=255) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTCGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt > 1:814467/1‑149 (MQ=255) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc < 1:361625/149‑1 (MQ=255) ttATCAGGCCTACGCCAGACAGCGCGATAGCCTGATTTAG‑CGTCATTTTGTAGGTCGGATAAGGCGTT‑‑TGCG‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGAGTCTTATAAGGCCTATCTTATCCGTTGGTTAATTTTTCa > 1:766466/1‑149 (MQ=14) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActc > 2:977778/1‑149 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 1:892917/149‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCTTAACCGTTGGTTAATTTTTCAAACTCtt < 2:773398/149‑1 (MQ=14) cTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTGTGTTAATTTTTCAAtctcgcca > 1:228980/1‑145 (MQ=11) cTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTATTca > 2:355560/1‑149 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc > 2:872690/1‑149 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc > 2:144207/1‑149 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAATCTTCACAcc > 2:805798/1‑149 (MQ=255) cAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg < 1:193574/149‑1 (MQ=255) cAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTCGGTTACTTTTTCACACTCTTCACCCCCg > 2:555401/1‑149 (MQ=14) aGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg < 1:83110/149‑1 (MQ=255) gACAGCGTAAAAGCCTAATATAG‑CGTGATTTTGTAGGCCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt < 2:432476/149‑1 (MQ=17) aCAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 1:946845/149‑1 (MQ=255) taaTAGCTTGATTTAG‑CGTGATTTTGTAGGTTGGATTAGGCGTT‑‑TTCG‑‑C‑C‑GCATACGACATCAATGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc < 2:344450/148‑1 (MQ=18) aaTAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc < 1:977778/149‑1 (MQ=255) aaTAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑A‑‑CGCAGCATCCGACATCAATGCCTGATGCAACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGCTACTTTTTCAAACTCTTCACACCCGGTACCAAAcc > 2:38371/1‑149 (MQ=17) gCCTGATTTAGCCGTG‑TTTTTTAGGTCATATAAGGCGTT‑‑T‑‑ACGC‑C‑GCTTCCGACATCAATGCCGTATTCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGTTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 2:650311/149‑1 (MQ=18) gCCTGATTTAG‑CGTTATTTTGTGGGTCCGACCCGGGGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 2:810355/149‑1 (MQ=21) gCCTGATTCAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:241010/149‑1 (MQ=255) tGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAAATCTTCACACCCGGTATCAAACCCTTCCAt > 2:237866/1‑149 (MQ=18) ttAG‑CGGGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAGCGCTTCACACCCGGTATCAAACCCTTCCATACAg < 2:263750/149‑1 (MQ=21) gTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCAACGATTCCGGTATCAAACCCTTCCATACAGCTCAg < 1:498252/149‑1 (MQ=18) ttttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAACGCATGATGCGACGCTTCCCGCGTCTAATCAGGCCTATCTTAACCGTTGGTGAATTTTTCAAACTCTTCTCACCCGGTATCAAACCCTGCCATACACCTCAgctca > 2:373151/1‑145 (MQ=18) ttGAAGGTCCGATAAGGCGTG‑‑T‑‑ACGC‑C‑GCCTCCGACATCAATGCCCGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa < 2:257142/149‑1 (MQ=25) tGTAGGTCGGATAAGGCGATACC‑‑A‑‑C‑C‑GCATCCGACATCAACGCCTCATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 1:182781/149‑1 (MQ=25) gTCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTg > 2:387442/1‑149 (MQ=255) tCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACACCCGTTATCAAACCCTTCCATACAACTCAGGTTCAACCatttc > 2:91380/1‑145 (MQ=25) tCGGATAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGCTGGTTAATTTTTCAAACTCTTCACACCCCGTATCAAACCCTTACATAAAGCTCCGGTTCAACAAGTGc > 1:464053/1‑149 (MQ=21) gATAAGGCGTT‑‑T‑‑ACGC‑C‑GTATCCGACATCAATGCCTGATGCGAAGCTGGCCGTCACTTATCAGGCCTATCGTTACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc < 1:445765/149‑1 (MQ=255) tAAGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc < 2:550751/149‑1 (MQ=255) aGGCGTT‑‑T‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt > 1:802869/1‑149 (MQ=255) || GACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAG‑CGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCT > NC_000913_3_bme_pgi/4296297‑4296519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |