| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 3,560,455 | 1 | . | G | 97.6% | 123.0 / ‑4.1 | 42 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (18/23); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT > NC_000913_3_bme_pgi/3560311‑3560584 | atatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cgcag > 2:896290/1‑145 (MQ=255) tCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt > 1:519470/1‑149 (MQ=255) tCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGAGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt > 2:215511/1‑149 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATc < 1:670071/149‑1 (MQ=255) aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGttt > 1:94020/1‑149 (MQ=255) ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCATGCCACGGCGTGTTA‑ACCGAACTGGAAAGCAGCGCCGCACCGCCATGATTG‑CGCACGATCAAGTTTTGCTCCGCCAGCTc > 1:780734/1‑149 (MQ=255) ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAt < 1:164663/149‑1 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 2:184714/149‑1 (MQ=255) ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg < 2:702290/149‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa > 1:774744/1‑149 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATAAAGG‑CGCAGGATAAGGGTTTGCTCCGCCGTCTCAATGAGGTcacg > 2:299254/1‑149 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg > 2:919426/1‑149 (MQ=255) gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 1:949288/140‑1 (MQ=255) gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTCAg > 2:949288/1‑140 (MQ=255) ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCCCCCCCAGCACATTGAGGTCGCGGCGa > 2:440906/1‑149 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTc < 2:653474/149‑1 (MQ=255) aTGCGCTCTTTTTCTGCGGTCTGGGTGGCCGTGCTGTCGTTCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt < 1:890301/149‑1 (MQ=255) cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACAAGGTACT‑TTCTGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc < 1:987853/149‑1 (MQ=255) ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCAGAGGGGAATAGTCTGCGGGCTGACgg > 1:936104/1‑149 (MQ=255) tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt < 2:461059/88‑1 (MQ=255) tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt > 1:461059/1‑88 (MQ=255) gtcGGGCAGGGTGGCCTTGCGATGGGGCCACGGCGGGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct < 2:84510/148‑1 (MQ=255) gtgcgTGGACTTGCTATCGTGCCACGGCGTGTCA‑ACCGAACTGGATGGCAGCGCCGCACCTCCATGATGG‑CGCAGCATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCa < 2:73323/145‑1 (MQ=255) tGGCCTTGCGATGCTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct < 1:327014/149‑1 (MQ=255) ccTTGCGATCGTGCCACGGCATGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg > 1:1000144/1‑149 (MQ=255) tGCGATCGTTCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCCCCGCTAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 1:514505/149‑1 (MQ=255) gATAGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCAGCGGGCTGACGGAGAAATCCCCTACCAGCTCTTCGGTACTg > 2:266883/1‑149 (MQ=255) cACGGCGTGTTA‑ACCGAACTGGACGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:486431/149‑1 (MQ=255) aCGGCGTGTTA‑ACCGAGCTGGAAGGCAGCGCCGCACCGTCATGATGGTG‑CATGATCAGGTTTTTCTCCGTCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc < 1:266883/149‑1 (MQ=255) cGTGTTA‑ACCGAACGTGAAGGCGGCGCCGCACCGCCATGATGG‑CGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:70461/149‑1 (MQ=255) gtgtTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg > 1:18354/1‑149 (MQ=255) tgtTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 1:947573/149‑1 (MQ=255) gtTA‑ACCGAATTGGAAGGAAGCGGCGCACCGCCATGATGG‑CGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGTCTGACGGAGAAATGCTCTATCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 1:333413/149‑1 (MQ=255) aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACAGACATAACCCTGCTGTTTAAc < 2:94020/149‑1 (MQ=255) cgtGGAAGGCAGGGACGCACGGCAATGCGGG‑CGCAGGATCAGGTTTTGCTCGGCCAGCGGATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:479901/147‑1 (MQ=255) gAAGGCCGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 1:111354/149‑1 (MQ=255) gAAGGCAGCGCCGCACCGCGATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCACGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTATCAGCCCTTCGGTACTGACATAACCCTTCTGTTTAACCAGTTCGat > 2:849995/1‑149 (MQ=255) gAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat > 2:811753/1‑149 (MQ=255) gAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 1:520822/149‑1 (MQ=255) aGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCCCCAGCTCATTGAGGCCGCGGCGAATAGTCTGCGGTCTGACGGAGAAATGATCTACCAGCTCTTCGGTACTGTCATACCCCTGCTGTTTAACCAGCTCGatga > 2:488363/1‑147 (MQ=255) gccgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt < 1:419562/149‑1 (MQ=255) tgatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc > 2:583956/1‑80 (MQ=255) tgatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc < 1:583956/80‑1 (MQ=255) | ATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTA‑ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT > NC_000913_3_bme_pgi/3560311‑3560584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |