Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 3,440,086 C→A G315V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,440,0860CA98.8% 274.8 / NA 81G315V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (40/40);  minor base G (0/1);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCA  >  NC_000913_3_bme_pgi/3439944‑3440232
                                                                                                                                              |                                                                                                                                                  
gCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAgaga                                                                                                                                             >  2:567762/1‑150 (MQ=255)
 ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGAc                                                                                                                                            >  1:580139/1‑150 (MQ=255)
         tGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACg                                                                                                                                    <  1:199237/150‑1 (MQ=255)
           gctgctGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACggg                                                                                                                                  >  1:48908/1‑150 (MQ=255)
             tggtgtgGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGaa                                                                                                                                <  2:478442/147‑1 (MQ=255)
             tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTGTTCCAGGCGCATGACCAGAGACAGTCCACGGGaa                                                                                                                                >  4:295763/1‑150 (MQ=255)
                tgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGcc                                                                                                                             <  4:235251/150‑1 (MQ=255)
                    gCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCa                                                                                                                         <  3:219237/150‑1 (MQ=255)
                     cGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGCCAGTCCACGGGAAGCCAGCAc                                                                                                                        >  4:357349/1‑150 (MQ=255)
                     cGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCAc                                                                                                                        >  1:444477/1‑150 (MQ=255)
                     cGGTGCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCcaccac                                                                                                                        >  2:31925/1‑150 (MQ=255)
                       gTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGt                                                                                                                      >  4:209473/1‑150 (MQ=255)
                        ttCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTc                                                                                                                     >  2:196582/1‑150 (MQ=255)
                         tCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCt                                                                                                                    <  1:402562/150‑1 (MQ=255)
                           aGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                                                                                                                  <  3:209473/150‑1 (MQ=255)
                             ttGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTaa                                                                                                                >  1:469935/1‑150 (MQ=255)
                              tGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAt                                                                                                               <  4:136819/150‑1 (MQ=255)
                               gacgacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtc                                                                                                              >  4:136946/1‑150 (MQ=255)
                                  gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                           >  4:370722/1‑150 (MQ=255)
                                  gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                           <  4:217266/150‑1 (MQ=255)
                                   acCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAg                                                                                                          <  3:362901/150‑1 (MQ=255)
                                       ctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAag                                                                                                      <  3:370722/150‑1 (MQ=255)
                                         ctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCGGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAagag                                                                                                    >  2:426148/1‑150 (MQ=255)
                                          ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAgaga                                                                                                   >  2:74311/1‑150 (MQ=255)
                                           tACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCCTGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAt                                                                                                  >  4:188936/1‑150 (MQ=255)
                                             cGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt                                                                                                <  3:231087/150‑1 (MQ=255)
                                                tGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttttt                                                                                             <  1:423691/150‑1 (MQ=255)
                                                 ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTa                                                                                            <  3:108831/150‑1 (MQ=255)
                                                     cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCaa                                                                                        >  3:221716/1‑150 (MQ=255)
                                                      aTGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAg                                                                                       <  3:168691/150‑1 (MQ=255)
                                                           cTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                  >  2:293229/1‑150 (MQ=255)
                                                            ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                                 <  3:273839/150‑1 (MQ=255)
                                                            ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                                 >  4:369142/1‑150 (MQ=255)
                                                                ccTTCTCAGTAAACCCTTAACCGGGGATCCGGTTACTTGTTAGCGATACTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCAAGCACGGCTTTATGTTCGGTAAGAGATGTTGTACCAGGGTTAgtagat                                                                             >  4:166427/1‑145 (MQ=255)
                                                                 cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACTGGAAGCCAGCACGTCGTTAATCTCAGGAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                            <  2:26306/150‑1 (MQ=255)
                                                                 cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                            >  4:374516/1‑150 (MQ=255)
                                                                  ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                           >  1:11428/1‑150 (MQ=255)
                                                                  ttCTCAGTAAAACCTTAACCTGGGATCCGGTTACTCGTCGGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                           <  2:303147/150‑1 (MQ=255)
                                                                    ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                         <  2:275899/150‑1 (MQ=255)
                                                                     tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                        <  3:166427/150‑1 (MQ=255)
                                                                      cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                       >  3:202728/1‑150 (MQ=255)
                                                                       aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                      >  2:218197/1‑150 (MQ=255)
                                                                       aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                      >  4:300639/1‑150 (MQ=255)
                                                                       aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGCTTAGGCGTTTTAAGGa                                                                      >  2:575214/1‑150 (MQ=255)
                                                                        gTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                     >  3:213226/1‑150 (MQ=255)
                                                                             aCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCaa                                                                >  1:567413/1‑150 (MQ=255)
                                                                              ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                               <  3:201909/150‑1 (MQ=255)
                                                                               cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                                              >  1:445734/1‑150 (MQ=255)
                                                                                 tAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTc                                                            <  4:213226/150‑1 (MQ=255)
                                                                                   aCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAGCCTCgg                                                          <  2:580139/150‑1 (MQ=255)
                                                                                       gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                      <  1:571223/150‑1 (MQ=255)
                                                                                          aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                             >  2:514655/1‑124 (MQ=255)
                                                                                          aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                             <  1:514655/124‑1 (MQ=255)
                                                                                          aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                                   <  1:74311/150‑1 (MQ=255)
                                                                                            ccGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTa                                                 <  1:28198/150‑1 (MQ=255)
                                                                                             cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAc                                                >  2:249675/1‑150 (MQ=255)
                                                                                                 tACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGa                                            <  1:355543/150‑1 (MQ=255)
                                                                                                  aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                           <  2:437163/150‑1 (MQ=255)
                                                                                                     cGTCAGCGATGCTTGCCGGCGGCCCGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTACGCGAACTTTCTCCTCGGTTAGGCGTTCTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCAc                                        <  2:359345/150‑1 (MQ=255)
                                                                                                      gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                       >  3:241080/1‑150 (MQ=255)
                                                                                                       tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                                      >  4:63402/1‑150 (MQ=255)
                                                                                                           cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGGCCGGCCACGGGAAGCCAGCACGTCTTTAATCTCAGGAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                                  >  2:126539/1‑150 (MQ=255)
                                                                                                           cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                                  >  1:546626/1‑150 (MQ=255)
                                                                                                              tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                               <  1:561118/150‑1 (MQ=255)
                                                                                                                   gCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                          <  2:48908/150‑1 (MQ=255)
                                                                                                                   gCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                          <  1:527703/150‑1 (MQ=255)
                                                                                                                       gTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCACAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                      >  3:137041/1‑150 (MQ=255)
                                                                                                                        tGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                     <  3:300639/150‑1 (MQ=255)
                                                                                                                          gCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                   <  1:567762/150‑1 (MQ=255)
                                                                                                                           ccAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                  <  1:196582/150‑1 (MQ=255)
                                                                                                                            cAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt                 >  3:166896/1‑150 (MQ=255)
                                                                                                                               ttttCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa              >  3:112259/1‑150 (MQ=255)
                                                                                                                                 taccAGGCGCATGACCAGAGATAGTCCACGGGAAGCCAGCACGTCTTTAACCTCAGTAAGAGTATTCTTACCAAGGTTAGTCGTTTTAAGGTACTCAACCTCTTTACGCTGTACTAGATCACCGTTATCGTGGATAGCTTCTGCTTTAAg            <  2:295730/148‑1 (MQ=255)
                                                                                                                                  tCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg           >  3:40278/1‑150 (MQ=255)
                                                                                                                                    cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa         <  3:369142/150‑1 (MQ=255)
                                                                                                                                    cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa         <  2:444477/150‑1 (MQ=255)
                                                                                                                                     aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg        >  2:222762/1‑150 (MQ=255)
                                                                                                                                      ggCGCATGGCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACGTCGGTAAGCTGTACCAGATCAACGATATAGTGGATAGCTTCTGCGTTAAGGCAGt       <  2:204849/150‑1 (MQ=255)
                                                                                                                                       gcgcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGtt      <  1:255881/150‑1 (MQ=255)
                                                                                                                                       gcgcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGtt      <  4:112259/150‑1 (MQ=255)
                                                                                                                                           aTGACCAGAGACAGTCCACGGGAAGCAAGCACGTATTTAATGTCATGAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCa  >  2:178859/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCA  >  NC_000913_3_bme_pgi/3439944‑3440232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑