Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,441,387	G→T	R97S (CGT→AGT)	rpsD ←	30S ribosomal subunit protein S4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,441,387	0	G	T	100.0%	150.6 / NA	44	R97S (CGT→AGT)	rpsD	30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/29);  total (15/29)

TGCTTACAACGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACGACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCA  >  NC_000913_3_bme_pgi/3441243‑3441529                                                                                                                                                 |                                                                                                                                               tGCTTACAACGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCtt                                                                                                                                           <  2:457727/150‑1 (MQ=255)    ttACAACGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCca                                                                                                                                        >  1:295203/1‑150 (MQ=255)    ttACAACGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCca                                                                                                                                        <  1:523563/150‑1 (MQ=255)         gcGTCATGCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCaga                                                                                                                                   <  4:113675/149‑1 (MQ=255)          cGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCagag                                                                                                                                  <  1:166084/150‑1 (MQ=255)                tCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACa                                                                                                                            <  3:327352/150‑1 (MQ=255)                    aCTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGtt                                                                                                                        <  2:562941/150‑1 (MQ=255)                      tAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGtttt                                                                                                                      <  2:562605/150‑1 (MQ=255)                        aCCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCa                                                                                                                    <  1:547409/150‑1 (MQ=255)                              tAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGgtg                                                                                                              <  3:140822/150‑1 (MQ=255)                              tAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGgtg                                                                                                              >  2:449650/1‑150 (MQ=255)                                 gaagCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCACCTACGGCAACCACAGTTGCACAGACTACCTTCCAGCACAGCAAACCGGTTTTAACCGGTGTTg                                                                                                           >  1:55123/1‑150 (MQ=255)                                  aagCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGc                                                                                                          <  3:389534/150‑1 (MQ=255)                                    gCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCt                                                                                                        <  4:114370/150‑1 (MQ=255)                                        tGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCa                                                                                                    <  3:234557/150‑1 (MQ=255)                                           tAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGAc                                                                                                 >  4:172231/1‑150 (MQ=255)                                                acacGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGtgct                                                                                            >  1:459926/1‑150 (MQ=255)                                                      ccGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGcttct                                                                                      <  3:60562/150‑1 (MQ=255)                                                      ccGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGcttct                                                                                      <  3:119951/150‑1 (MQ=255)                                                        gTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCttt                                                                                    <  3:179617/150‑1 (MQ=255)                                                        gTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGGTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCttt                                                                                    <  1:279101/150‑1 (MQ=255)                                                         tttACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTg                                                                                   <  3:318368/150‑1 (MQ=255)                                                          ttACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTgt                                                                                  <  3:20252/150‑1 (MQ=255)                                                              cATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTgtagta                                                                              >  2:300499/1‑150 (MQ=255)                                                                      cTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGa                                                                      >  2:361527/1‑150 (MQ=255)                                                                       tttATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGaa                                                                     <  2:295203/150‑1 (MQ=255)                                                                        tgaTGGCTAAGCAGCTGACGTGCCTCTGCACGAGTGGCACCGAAGCCCAGACGGTATACAACGTTGTCCAGACTACCTTGCAGCAGAGCCAACAGGCTTTCACCGGTGTTGCCTTTCAGAAGTGCTGCTTCTTTGTAGTAGTTACGGAAc                                                                    <  4:262752/148‑1 (MQ=255)                                                                            ggCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGAc                                                                <  2:499741/150‑1 (MQ=255)                                                                                 aCCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTCTACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTcc                                                           >  4:218093/1‑150 (MQ=255)                                                                                  ccAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCa                                                          >  3:210642/1‑150 (MQ=255)                                                                                   cAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAg                                                         <  2:493701/150‑1 (MQ=255)                                                                                       tGACGTGCTTCTGCACCAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGCTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCcaccacc                                                     >  2:311915/1‑149 (MQ=255)                                                                                               ttCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGAt                                             >  1:255776/1‑150 (MQ=255)                                                                                               ttCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGAt                                             <  1:449650/150‑1 (MQ=255)                                                                                                  tGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCa                                                                                                    <  2:579240/92‑1 (MQ=255)                                                                                                  tGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCa                                                                                                    >  1:579240/1‑92 (MQ=255)                                                                                                   gCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACgg                                         <  2:153299/150‑1 (MQ=255)                                                                                                                       cATACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTATTAGTTACGGAACTGACGCTTCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACg                     >  2:135371/1‑150 (MQ=255)                                                                                                                        aTACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCttt                                                                                    <  3:186702/86‑1 (MQ=255)                                                                                                                        aTACGGTATACAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCttt                                                                                    >  4:186702/1‑86 (MQ=255)                                                                                                                                  cAACGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCacac          >  3:143584/1‑150 (MQ=255)                                                                                                                                     cGTTGTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCAt       <  1:452812/150‑1 (MQ=255)                                                                                                                                         gTCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTc   <  4:97944/150‑1 (MQ=255)                                                                                                                                          tCCAGACTACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTca  <  1:574034/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               TGCTTACAACGTCATTCGGACTAACCTGATAAGAAGCGATGTTAACAACACGACCGTTTACCATAATTGCTTTATGGCTAACCAGCTGACGTGCTTCTGCACGAGTGGCACCGAAGCCCATACGGTATACAACGTTGTCCAGACGACCTTCCAGCAGAGCCAACAGGTTTTCACCGGTGTTGCCTTTCAGACGTGCTGCTTCTTTGTAGTAGTTACGGAACTGACGCTCCAGCACACCATAGATACGGCGAACTTTTTGCTTTTCACGCAACTGCACACCATAGTCA  >  NC_000913_3_bme_pgi/3441243‑3441529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑