Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,159,127 C→A A213E (GCG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,159,1270CA98.4% 211.1 / NA 63A213E (GCG→GAG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (34/28);  minor base T (1/0);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGC  >  NC_000913_3_bme_pgi/4158982‑4159273
                                                                                                                                                  |                                                                                                                                                  
aTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGAt                                                                                                                                                >  2:58633/1‑150 (MQ=255)
   ttGCTGGCTATCTAGGAAGAGCGCCCGTGGGCTGACCGCCAATGCGTAGCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTGACTGTGTGCGAGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGaa                                                                                                                                             <  4:363764/150‑1 (MQ=255)
      cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGAt                                                                                                                                          <  3:185123/150‑1 (MQ=255)
      cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGAt                                                                                                                                          >  4:114835/1‑150 (MQ=255)
       tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATa                                                                                                                                         >  2:270329/1‑150 (MQ=255)
         gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATaca                                                                                                                                       <  2:435668/150‑1 (MQ=255)
            atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATaca                                                                                                                                       >  2:262814/1‑147 (MQ=255)
            atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATaca                                                                                                                                       <  1:262814/147‑1 (MQ=255)
              ctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACtt                                                                                                                                  >  2:467891/1‑150 (MQ=255)
                  gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCcgc                                                                                                                              <  3:362417/150‑1 (MQ=255)
                      aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGa                                                                                                                          <  2:143987/150‑1 (MQ=255)
                            cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGcc                                                                                                                    <  3:213682/150‑1 (MQ=255)
                              tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTg                                                                                                                  >  2:224333/1‑150 (MQ=255)
                                 gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                                                                                               >  1:118884/1‑150 (MQ=255)
                                   gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGGTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc                                                                                                             >  2:73085/1‑150 (MQ=255)
                                      ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                                                                                          <  2:109951/150‑1 (MQ=255)
                                      ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                                                                                          <  1:1679/150‑1 (MQ=255)
                                         cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                                                                       <  2:32480/150‑1 (MQ=255)
                                          gAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcgc                                                                                                      <  4:167619/150‑1 (MQ=255)
                                          gAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcgc                                                                                                      <  3:114835/150‑1 (MQ=255)
                                            aTGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                                                                    <  2:423141/150‑1 (MQ=255)
                                                 tACAGATGGCCGATCTGGCAGGGGAAACACTGCTGATGATGGACGATGGCCACTGTTTGGGC‑AACAAGCAGGTGGGATCATGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                                                               <  3:229787/150‑1 (MQ=255)
                                                         gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                                                                                       <  3:136616/150‑1 (MQ=255)
                                                                tGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAACCTCTGCGCAACCTGGTGGCGGCAggccccg                                                                                >  2:122888/1‑145 (MQ=255)
                                                                  gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                                                              >  2:203209/1‑150 (MQ=255)
                                                                    aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGTTTTCTGTTTTGAAGCCGGGGTGGATGAAGATAGACCCTTCCGCGCGACGAGGCTGGAAAATCTGGGCAACATGGTGGCGGCAGGTAGCGGGag                                                                            >  1:145977/1‑149 (MQ=255)
                                                                     ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                                           <  2:129692/150‑1 (MQ=255)
                                                                       ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                         >  3:126388/1‑150 (MQ=255)
                                                                       ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                         >  1:76750/1‑150 (MQ=255)
                                                                       ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                         >  1:18070/1‑150 (MQ=255)
                                                                        gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                                        >  2:117826/1‑150 (MQ=255)
                                                                         aaaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                                       <  4:156466/150‑1 (MQ=255)
                                                                         aaaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                                       >  4:385436/1‑150 (MQ=255)
                                                                           aaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                                                                     <  3:366276/150‑1 (MQ=255)
                                                                               tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                                 <  4:196967/150‑1 (MQ=255)
                                                                               tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                                 >  4:97737/1‑150 (MQ=255)
                                                                                gctgATGCTGGAAGATGGTCACTGGTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGcc                                                                <  2:202855/150‑1 (MQ=255)
                                                                                  tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg                                                              <  3:244621/150‑1 (MQ=255)
                                                                                    aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcg                                                            <  4:204720/150‑1 (MQ=255)
                                                                                      gCTGGCAGATGGTCACTGTTTGCGCGATCACGCA‑ATGGGTTACTGATTTGAAGCCGGGGAGGAAGAAGGTACCCACTGCCGCTCGACCAGCCTTGAAGCTCGGCGCAACATGCAGTCGGCAGGTAGCGGCACCACTTTACTGCCAgcgcc                                                          >  2:335514/1‑149 (MQ=255)
                                                                                       cTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctg                                                         >  2:258197/1‑150 (MQ=255)
                                                                                        tGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                                        <  2:273870/150‑1 (MQ=255)
                                                                                                 gTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc                                               >  1:44552/1‑150 (MQ=255)
                                                                                                  tCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc                                              >  1:594447/1‑150 (MQ=255)
                                                                                                    aCTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCgg                                            >  1:3219/1‑150 (MQ=255)
                                                                                                     cTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGa                                           >  4:212901/1‑150 (MQ=255)
                                                                                                             gcgATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGa                                                                             <  2:295376/108‑1 (MQ=255)
                                                                                                             gcgATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGa                                                                             >  1:295376/1‑108 (MQ=255)
                                                                                                             gcgATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                   <  1:430953/150‑1 (MQ=255)
                                                                                                               gATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgc                                 >  3:257464/1‑150 (MQ=255)
                                                                                                                       a‑aTGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgt                         >  2:212445/1‑150 (MQ=255)
                                                                                                                                tCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATc                 >  3:124047/1‑150 (MQ=255)
                                                                                                                                    ttttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc             >  1:320112/1‑150 (MQ=255)
                                                                                                                                       tGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTg          >  1:36182/1‑150 (MQ=255)
                                                                                                                                        gAAGCCGGGGAGGATGACGGGTGACACTTCCGCGCGACCAGCCTGGAAACTCTGCACAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc         <  3:3243/150‑1 (MQ=255)
                                                                                                                                        gAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc         >  4:66630/1‑150 (MQ=255)
                                                                                                                                        gAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc         >  4:66654/1‑150 (MQ=255)
                                                                                                                                          aGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGTTGGGGTTGTTTATCTGCCGTGCAt       >  1:365405/1‑150 (MQ=255)
                                                                                                                                          aGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAt       >  4:264931/1‑150 (MQ=255)
                                                                                                                                           gCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt      >  3:182717/1‑150 (MQ=255)
                                                                                                                                            ccGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTa     <  2:247930/150‑1 (MQ=255)
                                                                                                                                            ccGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTa     >  1:247930/1‑150 (MQ=255)
                                                                                                                                               gggAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCCGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc  <  3:329997/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCA‑ATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGC  >  NC_000913_3_bme_pgi/4158982‑4159273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑