BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,233,772	G→T	intergenic (‑539/‑2135)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,233,772	0	G	T	100.0%	533.3 / NA	146	intergenic (‑539/‑2135)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (80/66); total (80/66)

Only 100 of 150 total aligned reads displayed.

CTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGT  >  NC_000913_3_bme_pgi/4233625‑4233914

cTAATAAATGCTTCACTGCGCTAAGGGACTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGGATTGCTAATGGCAAGAGACGctac                                                                                                                                               >  1:1104860/1‑150 (MQ=255)

 taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGACGAGTATTGCTAATGGCAAGAGACGctacg                                                                                                                                              >  1:774133/1‑150 (MQ=255)

 taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGctacg                                                                                                                                              >  3:332576/1‑150 (MQ=255)

 taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGctacg                                                                                                                                              >  3:754375/1‑150 (MQ=255)

   ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTTTTTTTTTTTTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGGGTATTGCTAATGGCAAGAGACGCTACGaa                                                                                                                                            >  2:1041657/1‑150 (MQ=255)

   ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGaa                                                                                                                                            >  3:860774/1‑150 (MQ=255)

   ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGaa                                                                                                                                            <  2:1310406/150‑1 (MQ=255)

       aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCtt                                                                                                                                        >  1:116656/1‑150 (MQ=255)

          cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGaa                                                                                                                                     <  2:527762/150‑1 (MQ=255)

          cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGaa                                                                                                                                     <  1:518526/150‑1 (MQ=255)

                tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACg                                                                                                                               >  3:229868/1‑150 (MQ=255)

                     tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGc                                                                                                                          >  2:466516/1‑150 (MQ=255)

                       aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaa                                                                                                                        >  3:665860/1‑150 (MQ=255)

                         ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAg                                                                                                                      <  1:1155084/150‑1 (MQ=255)

                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                                                     <  4:829959/150‑1 (MQ=255)

                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGc                                                                                                                     >  2:1027921/1‑150 (MQ=255)

                                 cTCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                                                                                              >  2:1377389/1‑150 (MQ=255)

                                  tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                             >  3:16057/1‑150 (MQ=255)

                                  tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                             >  1:106510/1‑150 (MQ=255)

                                  tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                             >  4:44735/1‑150 (MQ=255)

                                  tCAACATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                                                                                             >  1:952298/1‑150 (MQ=255)

                                    aaCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCa                                                                                                           >  4:361486/1‑150 (MQ=255)

                                     aCATTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAt                                                                                                          >  1:634360/1‑150 (MQ=255)

                                       aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                        <  1:1282582/150‑1 (MQ=255)

                                       aTTACGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTg                                                                                                        >  4:40074/1‑150 (MQ=255)

                                           cGCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCg                                                                                                    <  1:457024/150‑1 (MQ=255)

                                            gCTAACGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCgg                                                                                                   <  2:467186/150‑1 (MQ=255)

                                                aCGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                               <  3:397914/150‑1 (MQ=255)

                                                aCGGCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATc                                                                                               <  4:229868/150‑1 (MQ=255)

                                                  ggCACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                                                                                             <  1:131156/150‑1 (MQ=255)

                                                    cACTAAAACCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                           >  2:952009/1‑150 (MQ=255)

                                                         aaaCCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                           >  2:1492702/1‑145 (MQ=255)

                                                         aaaCCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                                                                                           <  1:1492702/145‑1 (MQ=255)

                                                          aaCCGTAACATTTTCTTGTGACTGGTGCTATAATCTTGCAAAGGCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTAAGAAGCTTGAAGAAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGTCAcgcgc                                                                                     <  4:62146/150‑1 (MQ=255)

                                                           aCCATTACATTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgcg                                                                                    >  4:229167/1‑150 (MQ=255)

                                                              attacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ata                                                                                <  2:1084457/147‑1 (MQ=255)

                                                               ttacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCGAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑atat                                                                               >  2:1009764/3‑150 (MQ=255)

                                                               ttacaTTTTTCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑atat                                                                               >  1:717784/3‑150 (MQ=255)

                                                                      tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGcgcgcg                                                                        <  4:328424/150‑1 (MQ=255)

                                                                      tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGcgcgcg                                                                        <  1:1377389/150‑1 (MQ=255)

                                                                      tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGcgcgcg                                                                        <  4:341311/150‑1 (MQ=255)

                                                                      tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGcgcgcg                                                                        <  1:353586/150‑1 (MQ=255)

                                                                      tttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGcgcgcg                                                                        <  1:79118/150‑1 (MQ=255)

                                                                       ttCTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCgg                                                                       >  2:1520549/1‑150 (MQ=255)

                                                                         cTGTGACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCgggg                                                                     <  2:106510/150‑3 (MQ=255)

                                                                          tgtgACTGGTGCTACAATCTTCCAAAGTCACAACTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAACTGAAAAAGCATAGGGCGGAACCGGCCACGCTCG‑ATATGGGAGCGGCAt                                                                    >  2:230534/1‑150 (MQ=255)

                                                                            tgACTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCGAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTc                                                                  <  1:1594456/150‑1 (MQ=255)

                                                                              aCTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAg                                                                                                                                           >  2:1633356/1‑76 (MQ=255)

                                                                              aCTGGTGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAg                                                                                                                                           <  1:1633356/76‑1 (MQ=255)

                                                                                gggtgcTACAATCTTCCAAAGTCACAATTCTCAACATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAgcg                                                              <  2:1077908/146‑1 (MQ=255)

                                                                                 ggtgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAgcgc                                                             <  1:1396811/147‑1 (MQ=255)

                                                                                      tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGAcc                                                        >  1:859322/1‑150 (MQ=255)

                                                                                         aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccg                                                     >  4:800206/1‑150 (MQ=255)

                                                                                         aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccg                                                     >  3:665754/1‑150 (MQ=255)

                                                                                           tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggc                                                   <  4:600930/150‑1 (MQ=255)

                                                                                           tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggc                                                   >  3:275232/1‑150 (MQ=255)

                                                                                             ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggccg                                                 <  3:46756/150‑1 (MQ=255)

                                                                                               ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑at                                                                                 <  2:1181691/116‑1 (MQ=255)

                                                                                               ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑at                                                                                 >  1:1181691/1‑116 (MQ=255)

                                                                                               ccAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACACTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGAGCGCGGCATTCAGAGCCGAGCccggcgggg                                               >  2:892145/1‑149 (MQ=255)

                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCaa                                                                                                                        <  2:876414/76‑1 (MQ=255)

                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGTGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                             >  2:1281262/1‑150 (MQ=255)

                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                             >  1:572052/1‑150 (MQ=255)

                                                                                                 aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTCGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTc                                             <  1:952009/150‑1 (MQ=255)

                                                                                                    gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                         <  1:1304762/88‑1 (MQ=255)

                                                                                                    gTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGTCATTCAGCGCCGACCCCGGCCGGTTCggg                                          >  4:97874/1‑150 (MQ=255)

                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAgc                                                               <  2:381403/126‑1 (MQ=255)

                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc                                         <  2:26386/148‑1 (MQ=255)

                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGa                                       <  3:361486/150‑1 (MQ=255)

                                                                                                         aaTTCTCAAACCCAGAAGAGTATTGCGAATGGGAAGGGACGCTACGAAGCTTGGAGCGACGGTAGCAACGCGGAAAAAGCATTGGGAGGAATCGGCGCCGAGCG‑ATGTGCGCGCGGCATTTAGCGCGGACCCCGGCCGGTTCGGGCGata                                     <  4:16057/150‑1 (MQ=255)

                                                                                                         aaTTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGata                                     >  2:1288709/1‑150 (MQ=255)

                                                                                                            tctcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAACAGAATTGAGCACCATACGCCCAGCGCG‑ATATGCACCAGGAATGCAACGACGACCCCGGCCGGTTCGGGCGATATTc                                  >  3:929854/1‑150 (MQ=255)

                                                                                                            tctcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                              >  4:735475/1‑138 (MQ=255)

                                                                                                            tctcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt                                              <  3:735475/138‑1 (MQ=255)

                                                                                                              tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc                                >  1:1331179/1‑150 (MQ=255)

                                                                                                               cAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                          <  2:1533965/139‑1 (MQ=255)

                                                                                                               cAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg                                          >  1:1533965/1‑139 (MQ=255)

                                                                                                               cAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGct                               >  1:23166/1‑150 (MQ=255)

                                                                                                                aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                                                                                         >  3:262266/1‑76 (MQ=255)

                                                                                                                aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGCATAT‑CGCGCggggc                                                                    <  2:1345415/112‑4 (MQ=255)

                                                                                                                aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctc                              >  1:526390/1‑150 (MQ=255)

                                                                                                                 aaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct                             <  4:275232/150‑1 (MQ=255)

                                                                                                                        aagaGTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGga                      >  1:639715/1‑150 (MQ=255)

                                                                                                                         agagTATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCAGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgat                     <  2:634360/150‑1 (MQ=255)

                                                                                                                            ttATTGCTAATGTCAAGGTACGCTACAAAACTTGAAGCAACACTCTCAAATGTTAAAAATCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGgatgat                  <  3:874605/149‑1 (MQ=255)

                                                                                                                             tATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtc                 <  2:717784/150‑1 (MQ=255)

                                                                                                                             tATTGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtc                 <  2:952298/150‑1 (MQ=255)

                                                                                                                               ttGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtctt               >  4:704298/1‑150 (MQ=255)

                                                                                                                                tGCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggccg                                                 >  4:933913/1‑115 (MQ=255)

                                                                                                                                 gCTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct             <  1:466516/150‑1 (MQ=255)

                                                                                                                                  cTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCa                                                                                                                         >  1:531320/1‑42 (MQ=255)

                                                                                                                                  cTAATGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCa                                                                                                                         <  2:531320/42‑1 (MQ=255)

                                                                                                                                    aaTGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggc                                                   <  3:485180/109‑1 (MQ=255)

                                                                                                                                    aaTGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGtt          >  1:266036/1‑150 (MQ=255)

                                                                                                                                      tGGCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCg        >  3:30773/1‑150 (MQ=255)

                                                                                                                                       ggCAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCccggc                                                   >  2:1112175/1‑106 (MQ=255)

                                                                                                                                         cAAGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACt     >  1:150171/1‑150 (MQ=255)

                                                                                                                                          aaGAGACGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTg    >  1:1218836/1‑150 (MQ=255)

                                                                                                                                            gagaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAGTCGGCCGCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGAGGATCTGCTGTTCGACTGGt  >  2:985471/1‑150 (MQ=255)

                                                                                                                                            gagaCGCTACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAGCTGCTGGTCGACTGGt  >  2:985424/1‑150 (MQ=255)

CTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCG‑ATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGT  >  NC_000913_3_bme_pgi/4233625‑4233914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`