Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,184,318 T→G F1025C (TTC→TGC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,184,3180TG93.8% 769.2 / 28.8 226F1025C (TTC→TGC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (8/6);  new base G (108/103);  total (117/109)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 226 total aligned reads displayed.

GCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGG  >  NC_000913_3_bme_pgi/4184173‑4184464
                                                                                                                                                 |                                                                                                                                                  
gctgtgctgGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCgag                                                                                                                                                >  2:1573000/1‑150 (MQ=255)
  tgtgctgGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGagaa                                                                                                                                              <  2:1201005/150‑1 (MQ=255)
         gTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTc                                                                                                                                       >  4:1269580/1‑150 (MQ=255)
          tAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCgag                                                                                                                                                >  1:1683858/1‑140 (MQ=255)
          tAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCg                                                                                                                                      <  3:671978/150‑1 (MQ=255)
                  ggCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGGACTGACACACGAGTGCGCGAAGAAACTCGAGGCGaaa                                                                                                                              >  2:754796/1‑150 (MQ=255)
                   gCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAAc                                                                                                                             >  3:519730/1‑150 (MQ=255)
                     gTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAAcgc                                                                                                                           >  3:403372/1‑150 (MQ=255)
                         aaGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGagaaga                                                                                                                                            >  1:295321/1‑129 (MQ=255)
                         aaGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGagaaga                                                                                                                                            <  2:295321/129‑1 (MQ=255)
                         aaGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCa                                                                                                                       >  2:93358/1‑150 (MQ=255)
                           gCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCaaa                                                                                                                     >  1:1041202/1‑150 (MQ=255)
                                gaAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCAc                                                                                                                >  3:477759/1‑150 (MQ=255)
                                 aaGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCAcc                                                                                                               <  1:93358/150‑1 (MQ=255)
                                   gCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCga                                                                                                                                                 <  3:1102823/114‑1 (MQ=255)
                                   gCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACt                                                                                                                                        >  4:553606/1‑123 (MQ=255)
                                    cTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAg                                                                                                            >  2:1756327/1‑150 (MQ=255)
                                       gACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCgaga                                                                                                                                               <  2:348244/112‑1 (MQ=255)
                                       gACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCgaga                                                                                                                                               >  1:348244/1‑112 (MQ=255)
                                       gACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGc                                                                                                         >  1:513770/1‑150 (MQ=255)
                                       gACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGc                                                                                                         >  3:1288375/1‑150 (MQ=255)
                                              tGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATc                                                                                                  <  2:132445/150‑1 (MQ=255)
                                                ccGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTg                                                                                                <  3:1407467/150‑1 (MQ=255)
                                                        tCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCggg                                                                                        <  4:477759/150‑1 (MQ=255)
                                                        tCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCggg                                                                                        >  1:1956601/1‑150 (MQ=255)
                                                             ggctggAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGc                                                                                   <  1:1802443/150‑1 (MQ=255)
                                                              gctggAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCt                                                                                  >  2:168892/1‑150 (MQ=255)
                                                                tggAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGa                                                                                <  4:1354385/150‑1 (MQ=255)
                                                                  gAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATTACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTgg                                                                                               <  1:653335/133‑1 (MQ=255)
                                                                   aGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGa                                                                             <  1:482596/150‑1 (MQ=255)
                                                                     cTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGAtt                                                                           >  1:400099/1‑150 (MQ=255)
                                                                     cTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGAtt                                                                           >  1:2203249/1‑150 (MQ=255)
                                                                      tGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTg                                                                          <  1:1573000/150‑1 (MQ=255)
                                                                      tGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTg                                                                          >  1:860108/1‑150 (MQ=255)
                                                                       gggCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGt                                                                         >  2:443591/1‑150 (MQ=255)
                                                                         gCCTGACAGACGAAGAGAAACAAAATCAGCGGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTa                                                                       <  4:1553412/150‑1 (MQ=255)
                                                                          ccTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTaa                                                                      >  1:2507166/1‑150 (MQ=255)
                                                                           cTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAg                                                                     <  1:2427811/150‑1 (MQ=255)
                                                                             gACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCga                                                                                                                                                 <  2:2550133/72‑1 (MQ=255)
                                                                             gACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGt                                                                   <  2:1392704/150‑1 (MQ=255)
                                                                             gACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGt                                                                   <  4:519730/150‑1 (MQ=255)
                                                                             gACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGt                                                                   <  1:279031/150‑1 (MQ=255)
                                                                              aCAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGta                                                                  >  2:887059/1‑150 (MQ=255)
                                                                              aCAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCAACCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGta                                                                  >  3:15128/1‑150 (MQ=255)
                                                                               cAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCt                                                                                                 >  2:2348080/1‑118 (MQ=255)
                                                                               cAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCt                                                                                                 <  1:2348080/118‑1 (MQ=255)
                                                                                aGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGtata                                                                >  4:1154652/1‑150 (MQ=255)
                                                                                aGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCgaga                                                                                                                                               <  3:216982/71‑1 (MQ=255)
                                                                                    gAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTg                                                            >  2:526193/1‑150 (MQ=255)
                                                                                     aaGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTgg                                                           <  4:445731/150‑1 (MQ=255)
                                                                                      agagAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGc                                                          <  2:2290404/150‑1 (MQ=255)
                                                                                       gagaAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGAt                                                                            <  4:1009640/131‑1 (MQ=255)
                                                                                             cAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTaaa                                                   <  4:1402380/150‑1 (MQ=255)
                                                                                              aaaaTCAGCTGGAACAGCTGGCTGANCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAAc                                                  >  1:190816/1‑150 (MQ=255)
                                                                                              aaaaTCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAAc                                                  <  3:116738/150‑1 (MQ=255)
                                                                                               aaaTCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGc                                                          <  3:856551/141‑1 (MQ=255)
                                                                                               aaaTCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGc                                                          >  4:856551/1‑141 (MQ=255)
                                                                                                  tCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCg                                              <  2:860108/150‑1 (MQ=255)
                                                                                                  tCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCg                                              <  3:1269580/150‑1 (MQ=255)
                                                                                                     gCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGAt                                                                                                   >  4:510534/1‑94 (MQ=255)
                                                                                                     gCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTAt                                           <  2:1956601/150‑1 (MQ=255)
                                                                                                      cTGGAACAGCTGGCTGAGCAGTATGACGAATTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATc                                          <  2:1700438/150‑1 (MQ=255)
                                                                                                      cTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTAt                                           >  4:201975/1‑149 (MQ=255)
                                                                                                       tGGAACAGCTGGCCGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATcc                                         >  4:1283778/1‑150 (MQ=255)
                                                                                                        ggAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCa                                        <  2:1896384/150‑1 (MQ=255)
                                                                                                        ggAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCa                                        <  1:1201762/150‑1 (MQ=255)
                                                                                                         gAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGcg                                                                                                        <  1:1264315/85‑1 (MQ=255)
                                                                                                          aaCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGc                                      <  2:1251910/150‑1 (MQ=255)
                                                                                                           aCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGcc                                     >  1:379032/1‑150 (MQ=255)
                                                                                                              gctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGaa                                                                               >  4:381117/1‑105 (MQ=255)
                                                                                                              gctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGaa                                                                               <  3:381117/105‑1 (MQ=255)
                                                                                                               ctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGcc                                               >  3:1311130/1‑136 (MQ=255)
                                                                                                               ctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTgg                                  <  4:638358/149‑1 (MQ=255)
                                                                                                               ctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTgg                                  >  3:638358/1‑149 (MQ=255)
                                                                                                               ctggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGt                                 <  1:754796/150‑1 (MQ=255)
                                                                                                                 ggctgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGa                               >  2:1485569/1‑150 (MQ=255)
                                                                                                                    tgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGc                                                                                       <  1:2084402/91‑1 (MQ=255)
                                                                                                                    tgAGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCg                                                         >  2:67658/1‑121 (MQ=255)
                                                                                                                      aGCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGa                          <  1:169944/150‑1 (MQ=255)
                                                                                                                       gCAGTATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGAt                         <  2:400099/150‑1 (MQ=255)
                                                                                                                           tATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGACGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCa                     >  1:1114591/1‑150 (MQ=255)
                                                                                                                           tATGACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTgg                                  >  3:199480/1‑137 (MQ=255)
                                                                                                                              gACGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGgt                  >  2:1920448/1‑150 (MQ=255)
                                                                                                                                cGAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATcc                                         <  2:492939/125‑1 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGt                                                                         >  1:2062159/1‑92 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGt                                                                         <  2:2062159/92‑1 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGt                                 <  2:969263/132‑1 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGt                                 >  1:969263/1‑132 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGa                          <  2:2438052/139‑1 (MQ=255)
                                                                                                                                 gAACTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGgtcgt               >  4:14383/1‑150 (MQ=255)
                                                                                                                                  aaCTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCa                                        >  2:784451/1‑124 (MQ=255)
                                                                                                                                   aCTGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCa             <  1:887059/150‑1 (MQ=255)
                                                                                                                                     tGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGc                                                          >  1:1448888/1‑103 (MQ=255)
                                                                                                                                     tGAAACACGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGc                                                          <  2:1780203/103‑1 (MQ=255)
                                                                                                                                           acGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACa     >  2:2103437/1‑150 (MQ=255)
                                                                                                                                           acGAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTg                                   >  1:771249/1‑120 (MQ=255)
                                                                                                                                             gAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCg                                                         >  1:1760019/1‑96 (MQ=255)
                                                                                                                                             gAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTaaa                                                   >  4:1390084/1‑102 (MQ=255)
                                                                                                                                             gAGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAg   >  4:167720/1‑150 (MQ=255)
                                                                                                                                              aGTGCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCa                                        >  1:357895/1‑112 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACAGACGAAGAGAAACAAAATCAGCTGGAACAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGG  >  NC_000913_3_bme_pgi/4184173‑4184464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: