Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi526,7960CG21.7% 191.1 / 40.0 94A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (44/28);  new base G (9/11);  total (54/40)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTA  >  NC_000913_3_hsa_pgi/526648‑526943
                                                                                                                                                    |                                                                                                                                                   
cTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGCCAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGTCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAgcgc                                                                                                                                                     <  1:1004070/149‑1 (MQ=255)
 ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                    <  1:543347/149‑1 (MQ=255)
 ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                    >  1:568950/1‑149 (MQ=255)
   gcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                                                                  <  2:239570/149‑1 (MQ=255)
   gcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGa                                                                                                                                                  <  1:568142/149‑1 (MQ=255)
    cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                 <  1:1227166/149‑1 (MQ=255)
    cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                 <  1:205437/149‑1 (MQ=255)
    cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                                 <  1:714644/149‑1 (MQ=255)
     gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAat                                                                                                                                                >  1:673360/1‑149 (MQ=255)
      cAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGCAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAGACGGCAATACGGCGGGGAGCGAGGAata                                                                                                                                               >  1:577385/1‑149 (MQ=255)
       aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                              >  1:111536/1‑149 (MQ=255)
       aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                              <  2:435223/149‑1 (MQ=255)
         tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                            >  1:717042/1‑149 (MQ=255)
          gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGATGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                           <  1:462444/149‑1 (MQ=255)
          gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                           <  2:190995/149‑1 (MQ=255)
           ctggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCTCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgatg                                                                                                                                          <  1:401722/147‑1 (MQ=255)
              gCTGACGTTGGAGCAACGGGCCAGCCCGGTGGAGACGTTATACACACCGGCGCGAAAAGCTCTTGTTTATCACTGCGACCACCGGGGACTGCCTCTGGCGCTTATCAGCGAAGACGGAAATACGGAGTGGCGCGGGGAGTATGATGAAt                                                                                                                                       <  2:988938/149‑1 (MQ=11)
                  aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACAGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                   <  1:1163668/149‑1 (MQ=255)
                     gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCTCTGGCCCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCaa                                                                                                                                >  2:85407/1‑149 (MQ=255)
                      tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                               <  2:351051/149‑1 (MQ=255)
                        gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACCGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCa                                                                                                                             <  2:404883/149‑1 (MQ=255)
                         aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAg                                                                                                                            >  2:727045/1‑149 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATTCGGCGTGGAGCGCGGAAGATGATGAATGGGGCAACCAGc                                                                                                                           >  1:885997/1‑149 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                           <  1:1033218/149‑1 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                           >  2:445620/1‑149 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                           >  1:65803/1‑149 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                           >  1:244408/1‑149 (MQ=255)
                          gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCCTGGAGCGCGGAATATGATGAAAGGGGCAACCAGc                                                                                                                           >  1:942367/1‑149 (MQ=255)
                           cAACTGGCCAGACAGGGGTAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTTGAGCGCGGAATATGATGAATGGGGCAACCAGCt                                                                                                                          <  2:1024534/149‑1 (MQ=255)
                             aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTa                                                                                                                        >  2:551419/1‑149 (MQ=255)
                             aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                        <  1:41342/149‑1 (MQ=255)
                               tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                      <  2:1400634/149‑1 (MQ=255)
                               tGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACCGCAATACGGCGTGGCGCGGGGAGTAAGATGATTGGGGCAACCCGCATatt                                                                                                                      >  2:107034/1‑147 (MQ=11)
                                 gCCAGACAGGGTTAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCTGGGACTGCCGCTGGCGCTTATCAGCGAAGTCGGCATTACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCATCTTAATga                                                                                                                    <  2:70768/149‑1 (MQ=11)
                                   cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGATCATCTATATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                  <  1:1361229/149‑1 (MQ=11)
                                    aGACAGGTGGAGCCGGAATACACACCGGCGCGAAATGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                 <  2:70409/149‑1 (MQ=255)
                                    aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                 <  1:971193/149‑1 (MQ=255)
                                    aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                 <  1:1505918/149‑1 (MQ=255)
                                      aCAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATATGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGgaga                                                                                                               >  1:1204643/1‑149 (MQ=255)
                                        aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAATACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                             >  2:1324447/1‑149 (MQ=255)
                                        aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                             >  2:1330509/1‑149 (MQ=255)
                                          gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGCCGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTATTGAGGAGAAccc                                                                                                           >  2:1227117/1‑149 (MQ=11)
                                          gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                           >  1:271506/1‑149 (MQ=255)
                                           tGGAGCCGTAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCTGGGACTGCCGCTGGCGCTTATCAGCTAAGACGGCAATAAGGCGTTGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                          <  1:962464/149‑1 (MQ=17)
                                              aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                       >  1:423802/1‑149 (MQ=255)
                                               gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCACTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                      >  2:1071541/1‑149 (MQ=255)
                                                ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcc                                                                                                     >  1:1420733/1‑148 (MQ=255)
                                                ccGGAATACACACCGCCGCGAAAACCTCATCTTTAACACTGCGACCACCGTGGACTGCCGCTCACGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAAGGGCCAACCACCTTAAAGAGCACAACCCCcaaca                                                                                                     >  1:1438393/1‑149 (MQ=14)
                                                 cGGTATACACACCGGCGCGAACCGCTCATCTTTATCACTGCGACCACCGGTGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGTGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                                    <  2:360686/149‑1 (MQ=14)
                                                 cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                                    >  2:971715/1‑148 (MQ=255)
                                                   gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCGGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                  <  1:1179231/149‑1 (MQ=255)
                                                    aaTACATACCGTCGCGAACAGCTCATCTTTATCACTGCGACCCCCGGGGACGGCCGCTGGGGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGTTGATTGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctg                                                                                                 <  2:1282403/149‑5 (MQ=11)
                                                     aTACACACCGGCGCGAAAAGCTCATCTATATCACAGCGACCACCGGGTACTCCCGCAGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATgtgt                                                                                                <  1:987485/149‑1 (MQ=17)
                                                       acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCCGCTTAATGAGGAGAACCCGCATCATGTGTag                                                                                              >  2:1154958/1‑148 (MQ=255)
                                                         acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATcc                                                                                            >  2:956652/1‑148 (MQ=255)
                                                         acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGCAGCGCGGAATATGATGAAAGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATCTGTATCa                                                                                            >  1:90875/1‑149 (MQ=255)
                                                          cacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAg                                                                                           <  2:1520624/149‑1 (MQ=255)
                                                            ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCAGAATCATGTGTATCAgac                                                                                         >  1:32405/1‑147 (MQ=255)
                                                             cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCATCCg                                                                                        >  2:136639/1‑149 (MQ=255)
                                                                 gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                    >  2:720158/1‑149 (MQ=255)
                                                                   gAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCTGAATATGATGAATGGGGCCACCAGCTTAAGGAGGAGAACCCGCAGCATGTCTATCAGCCGAACCGt                                                                                  >  2:1073495/1‑149 (MQ=18)
                                                                         gcgatacttATCCCTGGGACCCCCCGGGTCTGCCGCTGGCGCTTATCAGCGAATCCGGCAATACGGCGGGGAGCGCGGTATATGTTGCATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                            <  2:1033213/142‑1 (MQ=17)
                                                                          tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                           >  1:207762/1‑149 (MQ=255)
                                                                            aTCTTTATCACTGCGACCACCGGGGACTTCCGATGTCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGGCGTACCGTCTGCCAggg                                                                         <  1:1429318/149‑1 (MQ=255)
                                                                             tCTTTATCACTGCGCCCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGATTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                        >  2:67794/1‑149 (MQ=255)
                                                                             tCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgc                                                                        >  1:618313/1‑149 (MQ=255)
                                                                               tttATCACTGCGACCACCGGGGACTGCCGCTGGCGATTATCAGCGAAGACGGCAATACCGCGTGGAGCGCGGAAAATCATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATCTGTATCAGCCCAACCGTCTCCCAGGgcac                                                                      >  2:811169/1‑148 (MQ=17)
                                                                                       tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatga                                                              >  1:790017/1‑149 (MQ=255)
                                                                                         cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCACTACGGCGGGGAGCGCGGAAGAAGATGAAGGGGGCAACCCGCTTAAGGAGGAGAACCCGCATCATGTGGATCCGCCGGACCGTCAGCCACGGCAGCAGCatgagg                                                            >  2:1148814/1‑147 (MQ=255)
                                                                                         cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                            >  1:555804/1‑149 (MQ=255)
                                                                                              accGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGaa                                                       >  2:785788/1‑149 (MQ=255)
                                                                                                cGGGGACTGCCGCTGGCGCTTACCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                                     >  1:15055/1‑149 (MQ=255)
                                                                                                    gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                 <  2:568950/149‑1 (MQ=255)
                                                                                                       tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                              >  2:304551/1‑149 (MQ=255)
                                                                                                       tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                              >  1:1181267/1‑149 (MQ=255)
                                                                                                         ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                            >  2:542159/1‑149 (MQ=255)
                                                                                                         ccGCTGGCGCTTATCAGCGAAGACGGCAAGACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                            <  2:1405146/149‑1 (MQ=255)
                                                                                                            tctttgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCGGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                         >  2:176382/6‑149 (MQ=255)
                                                                                                            cTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCTACCAGCTTAATGAGGAGAACCCGCATCATGTGTATAAACCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACta                                         <  2:233153/149‑1 (MQ=255)
                                                                                                                 gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                    >  1:373195/1‑149 (MQ=255)
                                                                                                                 gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGAATGATGAGGAATAATGGCTGTACTATAAcc                                    >  2:309466/1‑149 (MQ=255)
                                                                                                                      tCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                               >  2:1089121/1‑149 (MQ=255)
                                                                                                                         gCGAAGCCGGCAATATGGCGTGGAGCGCGGAATATGATGAATCGGGCAACCAGCTTAATGAGGAGAACCCGCCTCATGTGTATCATCCGTACCGTCTGCCACGGCAGCAGCATGATGACGAATCAGGGCTGTACTAAAACCGTCACCgg                            >  2:1042236/1‑149 (MQ=17)
                                                                                                                         gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                            >  2:328732/1‑149 (MQ=255)
                                                                                                                         gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCCTCAcccg                            >  2:1101929/1‑147 (MQ=255)
                                                                                                                           gAAGACGGCAATATGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                          <  2:1204643/149‑1 (MQ=255)
                                                                                                                             aGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGCGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtact                        >  2:1301748/1‑149 (MQ=255)
                                                                                                                              gACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGAAACCAGCTTAATGAGGAGAACAAGCATCATGTGTATCAGCCGTACCGTATGCCAGGGCAGCAGCAGGATGAGGAATCAGGTCTGTACTATAACCGTCACCGGgacta                       >  2:968531/1‑149 (MQ=17)
                                                                                                                                 ggCAATACGGCGTGGAGCGCGGAATTTGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTCCCGTCTGCCAGGGCCGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGa                    >  2:740635/1‑149 (MQ=255)
                                                                                                                                   cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCTGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                  <  1:85407/149‑1 (MQ=255)
                                                                                                                                   cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                  >  2:50770/1‑149 (MQ=255)
                                                                                                                                     aTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCg                <  2:15055/149‑1 (MQ=255)
                                                                                                                                      tACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt               <  1:369297/149‑1 (MQ=255)
                                                                                                                                         ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTGTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc            <  1:136639/149‑1 (MQ=255)
                                                                                                                                         ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc            >  1:408605/1‑149 (MQ=255)
                                                                                                                                         ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAGCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc            <  1:720158/149‑1 (MQ=255)
                                                                                                                                              ggAGCGAGGAATATGATGAATGGTTCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCTTGTTGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg       <  2:673360/149‑1 (MQ=17)
                                                                                                                                                  cgcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGt   <  1:785788/149‑1 (MQ=255)
                                                                                                                                                   gcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGta  <  2:244408/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTA  >  NC_000913_3_hsa_pgi/526648‑526943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: