Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_pgi 3,440,086 C→A G315V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi3,440,0860CA98.8% 226.4 / NA 82G315V (GGC→GTC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (38/43);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGC  >  NC_000913_3_hsa_pgi/3439942‑3440231
                                                                                                                                                |                                                                                                                                                 
tAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCGGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCag                                                                                                                                               >  1:1502705/1‑149 (MQ=255)
   ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAgaga                                                                                                                                            <  2:6995/149‑1 (MQ=255)
     tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACa                                                                                                                                          <  2:749957/149‑1 (MQ=255)
        cGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGCTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTc                                                                                                                                       >  2:503438/1‑149 (MQ=255)
               tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAACTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCAAGGCGCATGACCAGAGACAGTCCACGGGa                                                                                                                                >  1:1565854/1‑149 (MQ=255)
               tgctgTTGAGTTTCAGTTGACGACCACTCTTACGATGGCTCATGACCTAATCCTTCTCAGTAAAACCTTAACCTGTGATCAGGATACTCGTCAGCGATGCTTGACGGTGGCCAGTTTTCCAGGCGCATTACCAGAGACAGTCCACGGGa                                                                                                                                >  2:31707/1‑149 (MQ=255)
                gctgTTGCGGTTCAGTTGACGACCACTGTTACGATGGCGCATGACCTTATCCTTCTCAGTATACGCTTAACGGGTGATCCGTTTACTGGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCTGAGACACTCCTCGGGaa                                                                                                                               <  1:1247646/149‑1 (MQ=255)
                 ctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAg                                                                                                                              >  2:1127913/1‑149 (MQ=255)
                  tgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATAACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGc                                                                                                                             >  1:1386630/1‑149 (MQ=255)
                             aGTTGACGTACACTCGTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGCTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                                  <  1:1083972/149‑1 (MQ=255)
                             aGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                                  <  2:1189479/149‑1 (MQ=255)
                              gTTGACGACCACTCTTACGATGGCGCATGACATTATACTTCACAGAAAAACCTTAACCTGTGATCCGGTTACTCGACAGCGATGCTTGCCGCTGGCCACATTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCCGCATCTCttt                                                                                                                 >  1:275814/1‑149 (MQ=255)
                                 gccgacCACTCTTACGCTGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAt                                                                                                              <  1:44259/147‑1 (MQ=255)
                                 gacgacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAt                                                                                                              >  1:257779/1‑149 (MQ=255)
                                   cgacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGGATGACCAGAGCCAGTCCAAGGGAAGCCAGCACGTCTTTAAtct                                                                                                            >  2:124921/1‑149 (MQ=255)
                                    gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGCCAGTCCACGGGAAGCCAGCACGTCTTTAAtctc                                                                                                           >  1:227851/1‑149 (MQ=255)
                                    gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                                                                                                                 >  1:761041/1‑143 (MQ=255)
                                    gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                                                                                                                 <  2:761041/143‑1 (MQ=255)
                                    gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtctc                                                                                                           <  1:616007/149‑1 (MQ=255)
                                     acCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                          <  2:248745/149‑1 (MQ=255)
                                       cACTCTTACGATGGCGCAAGACCTTATCGTTCTCAGTTAAACCTTAACCTGTGTTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGATAGTCCACGGGAAGCCAGCACGGCTTTAATCTCAGt                                                                                                        <  2:1419922/149‑1 (MQ=255)
                                          tctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTACCCTGTGATCCGGGTACTCGTCAGCGATGCTTGCCGGTGGCCAGTAATCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTaaa                                                                                                     >  1:710685/1‑148 (MQ=255)
                                          tctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAag                                                                                                     >  2:685662/1‑149 (MQ=255)
                                            ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCCCGGGAAGCCAGCACGTCATTAATCTCAGTAagag                                                                                                   >  1:1369957/1‑149 (MQ=255)
                                              aCGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCAGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAt                                                                                                 >  1:493431/1‑149 (MQ=255)
                                                gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt                                                                                               <  2:202348/149‑1 (MQ=255)
                                                gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAgtt                                                                                               >  1:509203/1‑149 (MQ=255)
                                                 aTGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttt                                                                                              <  2:1389371/149‑1 (MQ=255)
                                                 aTGGCGCATAACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttt                                                                                              <  2:1386630/149‑1 (MQ=255)
                                                   ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttttt                                                                                            <  2:1194317/149‑1 (MQ=255)
                                                    gcgcATGACCTTATCCTTCTCAGTAAAACTTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTa                                                                                           <  1:711396/149‑1 (MQ=255)
                                                      gcATGACCTTATCCTTCTCAGTAAATCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTAcc                                                                                         >  1:389134/1‑149 (MQ=255)
                                                              ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                 <  1:1208098/149‑1 (MQ=255)
                                                              ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATACTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                 <  1:805488/149‑1 (MQ=255)
                                                                 taattCTCAGAAAAACCTTAACCTGTGATCCGGTTACTCTACAGCGATGCTTGCCGCTCGCCAGTTATCCAGACGCATCACCAGAGACAGGCCACGGGTAACCAGCAAGTCATTAATCTCAGTAAGAGTATTTATAACAACGTTatgag                                                                              >  2:1050911/4‑145 (MQ=255)
                                                                   cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGTTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                            <  1:1167772/149‑1 (MQ=255)
                                                                   cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                            <  2:90909/149‑1 (MQ=255)
                                                                   cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGGTTTCCAGGCGCATGACCAGAGACAGTCCACGGGCAGCCAGCACGGCTTTAATCTCAGTAAGAGCTTTTTTACCAAGGTTAGGCGtt                                                                            >  2:1444928/1‑149 (MQ=255)
                                                                    ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGCCAGTCCACGGGAAGCCGGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                           >  2:673119/1‑149 (MQ=255)
                                                                    ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCATGGTTAGGCGttt                                                                           >  2:572621/1‑149 (MQ=255)
                                                                    ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTACCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCACAGTAAGAGATTTTTTACCAAGGTTAGGCtttt                                                                           >  1:728824/1‑149 (MQ=255)
                                                                     tctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                          <  1:510266/149‑1 (MQ=255)
                                                                       tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                        <  1:1127913/149‑1 (MQ=255)
                                                                         aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCTGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGCAAGCCAGCAAGTCTATAATCTCAGTAAGAGAGTATTTACCAAGGTAAGGCGTTTTAAgg                                                                      >  1:307336/1‑149 (MQ=255)
                                                                         aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACTAAGGTTAGGCGTTTTAAgg                                                                      >  1:485137/1‑149 (MQ=255)
                                                                           tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                    <  2:1132081/149‑1 (MQ=255)
                                                                                 cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGCCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                              >  2:1406692/1‑149 (MQ=255)
                                                                                   tAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCt                                                            <  2:641103/149‑1 (MQ=255)
                                                                                     aCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                          <  2:1502705/149‑1 (MQ=255)
                                                                                     aCCTGTGATCCCGGTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                          <  2:916279/149‑1 (MQ=255)
                                                                                     aCCTGTGAACCGGTTACTCCTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCGTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                          <  2:1157659/149‑1 (MQ=255)
                                                                                      ccTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                         >  2:1316539/1‑149 (MQ=255)
                                                                                        tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                       >  1:1449697/1‑149 (MQ=255)
                                                                                        tgtgATACGGTTACTAGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGTTCAACCTCGGTa                                                       <  2:54516/149‑1 (MQ=255)
                                                                                         gttaTCCGTGTACTCTGCAGCGATTCTTGCCGGTGGCCAGTTTTCACGGCGCATGAACAGCGACAGTCCACCGGAAGACAGCACGTCTTTAATATCAGTAAGAGATTTTTTACCAAGTTTAGTCGTTTTAAGGAGCTCAACCTCGTTAc                                                      <  2:580332/146‑1 (MQ=255)
                                                                                            aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGGCAGTTTTCGAGGCGCATGACCAGAGACAGTCCACGGGGAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCt                                                   >  1:1067154/1‑149 (MQ=255)
                                                                                             tCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                                  <  2:389134/149‑1 (MQ=255)
                                                                                               cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGACAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTa                                                <  2:999858/149‑1 (MQ=255)
                                                                                                ggTAACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAc                                               <  1:76513/149‑1 (MQ=255)
                                                                                                 gTTACTCGTCAGCGATGCTTGCCGGTGGCCAGGTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCCGCACGTCTTTAATCTCAGTAAGAGCTTTTTTACCAAGGTTAGGCGTTTTAAGGCGCTCAACCTCGGTACGCTGTAcc                                              >  2:1102139/1‑149 (MQ=255)
                                                                                                  ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCa                                             >  1:821811/1‑149 (MQ=255)
                                                                                                     cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                          <  2:257779/149‑1 (MQ=255)
                                                                                                      tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTATTCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                         >  1:196876/1‑149 (MQ=255)
                                                                                                      tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGCTc                                         >  2:1158670/1‑149 (MQ=255)
                                                                                                              gATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGatat                                 <  1:503438/149‑1 (MQ=255)
                                                                                                               aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGAtata                                >  2:647037/1‑149 (MQ=255)
                                                                                                               aTGCTTGCCGGTGGACAGTTTTCCAGTCGCATGACCAGAGACAGTCCACGGGAAGCCAGCTCGTCTTTAATCTCAGTAAGAGTTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGAtata                                <  2:1028727/149‑1 (MQ=255)
                                                                                                                tGCTTGCCGGTGGCGAGGTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                               <  1:954805/149‑1 (MQ=255)
                                                                                                                tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAATAGATTTTTTACCAAGGGTAGGCGGTTTAAGTAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                               <  1:525466/149‑1 (MQ=255)
                                                                                                                 gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                              <  2:1188467/149‑1 (MQ=255)
                                                                                                                  cTTGCCGGTGGCCAGTTTTCCATGCGCATTAACAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTg                             <  2:999006/149‑1 (MQ=255)
                                                                                                                            gCCAGTTTTCCAGTCGCATGACAAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGAGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGCATAGCTTCt                   >  2:405858/1‑149 (MQ=255)
                                                                                                                            gCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCt                   >  2:1469230/1‑149 (MQ=255)
                                                                                                                                gTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCtt               >  1:489332/1‑149 (MQ=255)
                                                                                                                                   ttCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa            >  1:527948/1‑149 (MQ=255)
                                                                                                                                     cctggCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTACTCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg          <  2:938196/146‑1 (MQ=255)
                                                                                                                                     ccAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg          <  2:1364161/149‑1 (MQ=255)
                                                                                                                                       cggCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCATCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa        <  2:1565854/148‑1 (MQ=255)
                                                                                                                                       aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa        >  2:490994/1‑149 (MQ=255)
                                                                                                                                          cgcATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACTAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGtt     <  2:485137/149‑1 (MQ=255)
                                                                                                                                             aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCGTTAAGGCAGTTAGc  >  2:883091/1‑149 (MQ=255)
                                                                                                                                             aTGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCGCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTGGc  <  1:403356/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGC  >  NC_000913_3_hsa_pgi/3439942‑3440231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑