Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi225,4670GT32.0% 18.6 / 14.5 25intergenic (+10/‑33)ileV/alaVtRNA‑Ile/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (10/7);  new base T (7/1);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTAC  >  NC_000913_3_hsa_pgi/225443‑225614
                        |                                                                                                                                                   
cACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaa                         >  1:356336/1‑149 (MQ=32)
 aCTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACTCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaa                        >  1:907946/1‑149 (MQ=17)
 aCTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaa                        >  1:1062807/1‑149 (MQ=32)
 aCTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaa                        <  1:90772/149‑1 (MQ=32)
     aGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaataaa                    >  1:910439/1‑149 (MQ=32)
       gCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCATGAGTACTGCGGTTAGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaaa                  <  1:201210/149‑1 (MQ=255)
       gCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATAGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaaa                  >  2:1589135/1‑149 (MQ=17)
         cTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGGAGTGGTTAAATAAAAAAt                >  1:437735/1‑149 (MQ=17)
          tACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATATAAAATa               >  1:913412/1‑149 (MQ=17)
          cttcAAATTTGCCGTGCAAATTTGAAGAGGTTTTATCTACATGTTATGGGGCTATAGCTCTGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGGTCGATGCCGCATAGCTCCACCTTCTCTGTAGTGGTTATATATAAAAtt               >  1:1638481/4‑148 (MQ=11)
           ttcAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATAc              >  2:247435/3‑149 (MQ=255)
           ttcAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATAc              >  2:135664/3‑149 (MQ=255)
           ttcAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTCGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACAATCTCTGTAGTGGTTAAATAAAAAATAc              >  2:1657644/3‑149 (MQ=11)
            ccAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACt             >  2:1033479/1‑149 (MQ=32)
            ccAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGATGGGAGAGCGCCTGCTTTTCACGCAGGAGGTCTGCGTTTCGATCCCGAATAGCTCCCACATCTCTGTAGTGGTTAAATAAAAAATACt             >  2:95853/1‑149 (MQ=11)
             cAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt            >  1:10446/1‑149 (MQ=255)
             cAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt            <  2:590129/149‑1 (MQ=32)
             cAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt            >  1:1010357/1‑149 (MQ=32)
              aaaTTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTc           >  1:782307/1‑149 (MQ=12)
              aaaTTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTc           >  2:1547295/1‑149 (MQ=12)
               aaTTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTGCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCa          <  2:204292/149‑1 (MQ=17)
                 tttGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCGCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCaga        <  1:1323162/149‑1 (MQ=17)
                    gCACTGCAAATTTTAAGAGGTTTTTAGTAAATTTTATGTTGCTATATCTCAGATGGGAGAGCGCATGATTTGCACGCAGGAGGTCTGAGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAgtg     <  1:1153177/149‑1 (MQ=11)
                       cGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTAc  <  2:221764/149‑1 (MQ=32)
                       cGGCAAATTTGAAGAGGTATTAACTACATGTTATGGGGCTATAGCTGAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTAc  <  2:693586/149‑1 (MQ=17)
                        |                                                                                                                                                   
CACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTAC  >  NC_000913_3_hsa_pgi/225443‑225614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: