Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_pgi 4,400,459 C→A N48K (AAC→AAA hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi4,400,4590CA98.2% 336.9 / NA 114N48K (AAC→AAAhfqglobal sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (65/47);  minor base T (0/2);  total (65/49)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGG  >  NC_000913_3_hsa_pgi/4400314‑4400604
                                                                                                                                                  |                                                                                                                                                 
gaATGGCAAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACg                                                                                                                                                <  2:685228/149‑1 (MQ=255)
   tGGCTATGGGGCTATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCTGTTTATATTTATTTGGTGATTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAATACGGTc                                                                                                                                             <  2:1558207/149‑1 (MQ=255)
    ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCa                                                                                                                                            <  2:1005897/149‑1 (MQ=255)
    ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCa                                                                                                                                            <  1:1425860/149‑1 (MQ=255)
       tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGcc                                                                                                                                         >  2:805123/1‑149 (MQ=255)
          ggggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATTGTATTAAGCTGCAAGGGCAAATCGTGTCTATTGATCAGTTAGTGATCCTGTTGAAAAAAACGGTAAGCAAGa                                                                                                                                      >  1:703715/1‑149 (MQ=255)
           gggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGAt                                                                                                                                     <  1:1654165/149‑1 (MQ=255)
             gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTGTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGAtcg                                                                                                                                   >  2:771141/1‑147 (MQ=255)
             gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCATATgg                                                                                                                                   <  2:1318283/149‑1 (MQ=255)
              cAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGt                                                                                                                                  <  2:242149/149‑1 (MQ=255)
                  cTTTACAAGATCCGTGCCTGAACGCCCTGCGTCTGTAACGTGTTCCAGTTTCAATTTATTTGGTGAATGTTATTAAGCTGCAAGGTCAACTCGCGGCTTTTTATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTAc                                                                                                                              <  1:1067933/149‑1 (MQ=255)
                   tttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCTAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACa                                                                                                                             >  2:344098/1‑149 (MQ=255)
                   tttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGGTTCTATTTATTTGGTGAATGGTATTAAGCGGCAAGGGCAAAGCGAGTCTTTTGATCAGTTCGTGCTCCTGTTGAAAACAACGGTCAGCCAGATGGTTTAcc                                                                                                                             >  2:1279349/1‑148 (MQ=255)
                    ttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACaa                                                                                                                            >  2:721174/1‑149 (MQ=255)
                       cAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCa                                                                                                                         >  1:598703/1‑149 (MQ=255)
                          gATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCAcgc                                                                                                                      >  1:1207558/1‑149 (MQ=255)
                          aacacGTTCCCGACGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATCTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCCGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCAcgc                                                                                                                      >  1:547461/5‑149 (MQ=255)
                            tCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGa                                                                                                                    >  1:444958/1‑149 (MQ=255)
                               gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                                                                                                                 >  1:471884/1‑149 (MQ=255)
                               gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                                                                                                                 <  1:1353225/149‑1 (MQ=255)
                                gttcccgaCGCAGTGCGTTCAGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCATGTCGTGATCCTGTTGAAAAAAACGGTCAGCCACATGGTTTACAAGCACGCGAttt                                                                                                                 >  1:1651291/8‑149 (MQ=255)
                                  ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTa                                                                                                              <  1:1435532/149‑1 (MQ=255)
                                   cTGATCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGGGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                                                                                             <  1:1010862/149‑1 (MQ=255)
                                   cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                                                                                             <  2:676076/149‑1 (MQ=255)
                                   cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                                                                                             >  2:1417937/1‑149 (MQ=255)
                                   cTGAACGAACTGCGTCGGGAACGTGTTCCAGTTTCTATTATTTTGGTGAATGGTATTACGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAATAAACCGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                                                                                             <  2:1274597/149‑1 (MQ=255)
                                    tGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGACTTTTTATAAGTTCTAGATCCTGTTGAAAAAAACGGTCAGCAAGATGGTTTACAAGCACCCGATTTCTACt                                                                                                            >  1:223877/1‑149 (MQ=255)
                                       aCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGATTGGTATTATGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTAAAAGCACGCGATTTCTACtgtt                                                                                                         >  2:594300/1‑149 (MQ=255)
                                         gCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGTCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttgt                                                                                                       <  1:1568045/149‑1 (MQ=255)
                                               cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc                                                                                                 <  1:720542/149‑1 (MQ=255)
                                               cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGCCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc                                                                                                 <  1:1273725/149‑1 (MQ=255)
                                                     ggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCCAGGGCAAATCGAGTCTTTTGATCAGGTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGcc                                                                                            <  2:1280255/149‑1 (MQ=255)
                                                         cGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAATACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGt                                                                                        <  1:67522/149‑1 (MQ=255)
                                                           tgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGttt                                                                                      <  1:456873/149‑1 (MQ=255)
                                                             ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                                                    >  1:226975/1‑149 (MQ=255)
                                                             ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                                                    <  1:17541/149‑1 (MQ=255)
                                                              tCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAACGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtctc                                                                                   <  1:1236338/149‑1 (MQ=255)
                                                                cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACTCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcat                                                                                 >  1:1397619/1‑149 (MQ=255)
                                                                    ttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcaca                                                                             <  2:471884/149‑1 (MQ=255)
                                                                       tatttattTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTa                                                                          <  1:560101/149‑1 (MQ=255)
                                                                       tatttattTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTa                                                                          >  2:571071/1‑149 (MQ=255)
                                                                       tatttattTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATACTGTTGAAAAAAACTGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTGCTCATAACAGTa                                                                          >  2:1233677/1‑149 (MQ=255)
                                                                         tttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTATCGCCCGGTTTCTCATCACATTaac                                                                        >  2:1344212/1‑149 (MQ=255)
                                                                            atttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaccaac                                                                     >  2:149641/1‑149 (MQ=255)
                                                                            atttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaac                                                                     >  1:801834/1‑149 (MQ=255)
                                                                             tttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCTTCTCGCCCGGTTTCTCATCAGAGTAACAACg                                                                    >  2:591428/1‑149 (MQ=255)
                                                                               tGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGcc                                                                  <  2:1442879/149‑1 (MQ=255)
                                                                                ggTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCg                                                                 >  1:934605/1‑149 (MQ=255)
                                                                                  tGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGt                                                               >  1:580326/1‑149 (MQ=255)
                                                                                  tGAATGGTATTAAGCTGCAAGGGCAAATCGAGGCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGt                                                               <  1:78802/149‑1 (MQ=255)
                                                                                    aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGTTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCTGTgg                                                             >  1:936183/1‑149 (MQ=255)
                                                                                     aTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                                                            >  2:1418383/1‑149 (MQ=255)
                                                                                      tGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                                                           <  1:1355003/149‑1 (MQ=255)
                                                                                      aggTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                                                           <  2:790878/148‑1 (MQ=255)
                                                                                       ggTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACTACGCCGGTGGCgg                                                          <  1:721174/149‑1 (MQ=255)
                                                                                        gTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCAGGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTAATCACAGTAACAACGCCGGTGGAGGt                                                         >  1:123938/1‑149 (MQ=255)
                                                                                        gTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt                                                         >  2:79050/1‑149 (MQ=255)
                                                                                         tATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTACCGTCTCGCCCGGTTACTCAACACAGAAACAACGCCGGTGGAGGTa                                                        >  2:302987/1‑149 (MQ=255)
                                                                                          aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTGATCCCAGTAACAACGCCGGTGGCGGTAc                                                       >  2:666717/1‑149 (MQ=255)
                                                                                          aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGtcc                                                       >  1:1489084/1‑147 (MQ=255)
                                                                                          aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTAc                                                       >  1:425260/1‑149 (MQ=255)
                                                                                           ttAAGCTGCAAGGGCAGATCGAGTCTTTTGATCAGTTCGTGATCCTGTGGAAACAAACGGGCAGCCAGAGGGTTTACAAGCCCGCGATTTCTACTGTTGTCGCGTCTCGCCCGGTTACTCATAACAGTAACAACGGCGGTGGCGGTAcc                                                      >  2:349023/1‑149 (MQ=255)
                                                                                             aaGATGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcag                                                    <  1:760318/149‑1 (MQ=255)
                                                                                               gCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCCGTTACTCATCACAGTAACAACGCCGGTGGCGGTCCcagca                                                  >  1:475408/1‑149 (MQ=255)
                                                                                                cTGCATAGGCAAATCGAGTCTCTTGATCAGTCCGTCATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagcag                                                 <  2:915228/149‑1 (MQ=255)
                                                                                                cTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagcag                                                 >  1:577024/1‑149 (MQ=255)
                                                                                                      gggCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTa                                           >  1:45505/1‑149 (MQ=255)
                                                                                                        gCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTAcc                                         >  2:683615/1‑149 (MQ=255)
                                                                                                           aaTCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatc                                      <  1:1417937/149‑1 (MQ=255)
                                                                                                               gAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCCGCAGTACCTACCAACATgg                                  >  2:391581/1‑149 (MQ=255)
                                                                                                               gAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGGCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGTCGGTACCAGCAGTAACTACAATCATgg                                  >  2:1451452/1‑149 (MQ=255)
                                                                                                                 gTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcag                                                    <  2:1363434/129‑1 (MQ=255)
                                                                                                                 gTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcag                                                    >  1:1363434/1‑129 (MQ=255)
                                                                                                                 gTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTa                                <  1:381412/149‑1 (MQ=255)
                                                                                                                  tCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTTGCGGTACCAGCAGTAACTACCATCATGGTag                               >  2:7276/1‑149 (MQ=255)
                                                                                                                  tCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTag                               <  2:444958/149‑1 (MQ=255)
                                                                                                                    ttttGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACCAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTACCAACGCCGCTGGCGGTACCAGCAGTAACTACGCTCATGGTagca                             >  2:55958/1‑149 (MQ=255)
                                                                                                                    ttttGATCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAAAGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagca                             >  1:833752/1‑149 (MQ=255)
                                                                                                                    gtttGATCAGTTCGTGATCCTGGTGAAAAAAACGGTCAGCCAGATGGGTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGATTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagcc                             >  2:1555765/2‑148 (MQ=255)
                                                                                                                         aTCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcg                        >  1:251705/1‑149 (MQ=255)
                                                                                                                         aTCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcg                        >  1:531447/1‑149 (MQ=255)
                                                                                                                         aTCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcg                        <  2:598703/149‑1 (MQ=255)
                                                                                                                         aTCACTTCGTGATCCTGTTGAAAACAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGTAGTAACTCCCATCATGGTAGCAgcgcg                        <  2:1643196/149‑1 (MQ=255)
                                                                                                                          tCAGTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcgc                       >  2:422951/1‑149 (MQ=255)
                                                                                                                             gTTCGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGa                    >  1:466686/1‑149 (MQ=255)
                                                                                                                                cGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                                                            >  1:1658746/1‑106 (MQ=255)
                                                                                                                                cGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                                                            <  2:1658746/106‑1 (MQ=255)
                                                                                                                                cGTGATCCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATa                 <  1:771141/149‑1 (MQ=255)
                                                                                                                                 gTGATCCTGTTGAAAAAAACGGGCATCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGGCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATAc                <  2:1259418/149‑1 (MQ=255)
                                                                                                                                  tGATCCTGTTGAAAAAAACGGGCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACt               <  1:1279349/149‑1 (MQ=255)
                                                                                                                                     tCCTGTTGAAAAAAACGGTCAGCCAGATGGGTTACAAGCACGCGATTTCTACTGTTGTCCCGTCGCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGGACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTcc            <  1:1295037/149‑1 (MQ=255)
                                                                                                                                        tGTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCcgcg         >  2:1053129/1‑149 (MQ=255)
                                                                                                                                         gTTGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCgcgc        >  2:247705/1‑149 (MQ=255)
                                                                                                                                          ttGAAAAAAACGGTCAGCCAGCTGGTTTACAAGCCCGCGAGTTCTACTGTTGTCCCGTCTCGCCCGGTGTCTCAGCCCAGTAACAACGCCGGTGGCGGTACCGGCAGTAACTACCCTCATGGTAGCAGCGCGCAGAATACTGCCGCGCa       >  2:343584/1‑149 (MQ=255)
                                                                                                                                          ttGAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCa       >  1:1220249/1‑149 (MQ=255)
                                                                                                                                            gAAAAAAACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGGTTCTCAGCCCAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAGACGTCCGCGCAAc     >  1:37284/1‑149 (MQ=255)
                                                                                                                                             aaaaaaACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACa    >  1:70839/1‑149 (MQ=255)
                                                                                                                                             aaaaaaACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAAACTTACGCCCAACa    >  2:576753/1‑149 (MQ=255)
                                                                                                                                              aaaaaaCGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAg   >  2:1115845/1‑149 (MQ=255)
                                                                                                                                               aaaaACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAgg  >  2:1357924/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATACTTCCGCGCAACAGG  >  NC_000913_3_hsa_pgi/4400314‑4400604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: