Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_pgi	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_pgi	1978503–1979269	1979278	10–776	41 [1]	[0] 40	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_pgi	= 1978502	0 (0.000)	33 (0.660)	30/254	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_pgi	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_pgi/1978357‑1978650                                                                                                                                                  |                                                                                                                                                     aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                                   >  1:738083‑M1/1‑146 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                          >  1:974916‑M1/1‑137 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                          <  1:841218‑M1/149‑13 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                          <  1:506406‑M1/149‑13 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                                   >  2:43173‑M1/1‑130 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAAGAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                                   <  1:641542‑M1/149‑20 (MQ=255)                     cTAAGATTTTTTCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAATCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  2:508362‑M1/149‑24 (MQ=255)                      tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                              >  2:355979‑M1/1‑125 (MQ=255)                        aGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                                                                            >  1:528457‑M1/1‑123 (MQ=255)                                 cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgtgtgtatttcaat                                                                                                                   >  1:76163‑M1/1‑114 (MQ=255)                                  tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCAATGTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttgtggtgtatttcaatc                                                                                                                  >  2:100991‑M1/1‑113 (MQ=255)                                                 ggactCGTCACTACACGCACATAAAATGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                   <  2:984233‑M1/147‑52 (MQ=255)                                                   aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                                 <  1:803717‑M1/149‑54 (MQ=255)                                                     tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGGTGCAGATCACACAAAACACTCAATTACTTAACATAAATGgataagtcattcttttgttttgggtgtatttcaatctgttaaaaagttttttgct                                                                                               >  1:155898‑M1/1‑94 (MQ=255)                                                     tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtaataattttgttttgggtgtatttcaatctgttaaaaaatttttcgct                                                                                               >  1:660936‑M1/1‑94 (MQ=255)                                                       gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATACATTtataagtcatacttttgttttgggtgtatttcaatctgtttaaaagtttttcactct                                                                                             >  2:417705‑M1/1‑92 (MQ=255)                                                                  cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagca                                                                                  >  2:908038‑M1/1‑81 (MQ=255)                                                                      tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                              <  1:355979‑M1/149‑73 (MQ=255)                                                                         aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                           >  2:489024‑M1/1‑74 (MQ=255)                                                                          aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaa                                                                          >  1:247521‑M1/1‑73 (MQ=255)                                                                              aGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                      <  1:753001‑M1/149‑81 (MQ=255)                                                                                   ggCTGCGATTTTCAATAATGCGTGATGCAGATCACACTAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtttgaaagtttttcgctacgctagcaagcaaaaatgaaacaggaata                                                                 <  1:213039‑M1/149‑86 (MQ=255)                                                                                             ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgg                                                       <  1:649003‑M1/149‑96 (MQ=255)                                                                                                aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                                    >  1:412424‑M1/1‑51 (MQ=255)                                                                                                 ataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatg                                                   <  1:629406‑M1/149‑100 (MQ=255)                                                                                                          gATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttgggcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                          <  1:897391‑M1/149‑109 (MQ=255)                                                                                                              cAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtca                                      >  2:665865‑M1/1‑37 (MQ=255)                                                                                                                gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaa                                    >  1:1002610‑M1/1‑35 (MQ=255)                                                                                                                 aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                                   <  2:738083‑M1/149‑116 (MQ=255)                                                                                                                    acacaAAACACTGCATTACTTAACATAAATGtataagttatacttttgttttgggtgtatttcaagctgttaaaaagtttttcgctacgctagcaagcaaaaatgacacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                                <  2:243524‑M1/149‑119 (MQ=255)                                                                                                                        aaaaCACTTAATTACTTAACATAAATTtataagtcatacttttgttttgggtggatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                            <  1:247240‑M1/149‑123 (MQ=255)                                                                                                                        aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                            <  2:247521‑M1/149‑123 (MQ=255)                                                                                                                          aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcccgcaaacatgaaacagaaataatcgaaatgggatgttgcgcacagtcaaaataactcacc                          >  2:862078‑M1/1‑25 (MQ=255)                                                                                                                                    tACTCAACATTAATGtacaagtcgtacttttggcttgggttcatttcaatctgtgaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggcatcatcgaaatgggctgttgcgcacagtcaaaataacgcaccgcaaataatc                <  2:18722‑M1/149‑135 (MQ=255)                                                                                                                                       ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgcgaagaaggtgttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaacgtaaataatcatc             >  1:195579‑M1/1‑12 (MQ=255)                                                                                                                                       ttAACATAAATGtataagtcatacttccagattttgtgtattgcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgaacagtcaaaatacctcacagtaaataatcatc             >  2:721270‑M1/1‑12 (MQ=255)                                                                                                                                         gggtggaaTGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGc           <  2:998811/143‑1 (MQ=17)                                                                                                                                          aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaattggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc          <  1:908038‑M1/149‑141 (MQ=255)                                                                                                                                                aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgagcacagtcaaaataactcaccatgaataatcatctgctctaaa    >  2:229865‑M1/1‑3 (MQ=255)                                                                                                                                                 tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaat   <  1:489024‑M1/149‑148 (MQ=255)                                                                                                                                                  gtataagtcatacttttgttttgggtgtatttaaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgatgcgcacagtcaaaataactcaccgtaaataatcatctcctataaata  >  2:180679‑M1/1‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_hsa_pgi/1978357‑1978650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑