mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SvNSRound4_19_9_S2396_L007_R1_001.good.fq | 7,429 | 919,696 | 100.0% | 123.8 bases | 150 bases | 97.4% |
| errors | SvNSRound4_19_9_S2396_L007_R2_001.good.fq | 7,429 | 919,696 | 100.0% | 123.8 bases | 150 bases | 97.1% |
| errors | SvNSRound4_19_9_S2396_L008_R1_001.good.fq | 4,574 | 565,058 | 100.0% | 123.5 bases | 150 bases | 96.8% |
| errors | SvNSRound4_19_9_S2396_L008_R2_001.good.fq | 4,574 | 565,058 | 100.0% | 123.5 bases | 150 bases | 95.7% |
| total | 24,006 | 2,969,508 | 100.0% | 123.7 bases | 150 bases | 96.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913_3_KO_pgi | 4,640,086 | NA | NA | 100.0% | Escherichia coli str. K-12 substr. MG1655, pgi gene KO, complete genome. |
| total | 4,640,086 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
Insufficient coverage Reference sequence counted as entirely deleted due to low coverage. Try either the -t,--targeted-sequencing or the -c,--contig-reference option if you want mutations called for these reference sequences.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 682 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 111 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.007 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913_3_KO_pgi | 0.99758 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 17:41:50 19 Dec 2020 | 17:41:55 19 Dec 2020 | 5 seconds |
| Read alignment to reference genome | 17:41:56 19 Dec 2020 | 17:42:18 19 Dec 2020 | 22 seconds |
| Preprocessing alignments for candidate junction identification | 17:42:18 19 Dec 2020 | 17:42:19 19 Dec 2020 | 1 second |
| Preliminary analysis of coverage distribution | 17:42:19 19 Dec 2020 | 17:42:26 19 Dec 2020 | 7 seconds |
| Identifying junction candidates | 17:42:26 19 Dec 2020 | 17:42:26 19 Dec 2020 | 0 seconds |
| Re-alignment to junction candidates | 17:42:26 19 Dec 2020 | 17:42:30 19 Dec 2020 | 4 seconds |
| Resolving best read alignments | 17:42:30 19 Dec 2020 | 17:42:32 19 Dec 2020 | 2 seconds |
| Creating BAM files | 17:42:32 19 Dec 2020 | 17:42:34 19 Dec 2020 | 2 seconds |
| Tabulating error counts | 17:42:34 19 Dec 2020 | 17:42:35 19 Dec 2020 | 1 second |
| Re-calibrating base error rates | 17:42:35 19 Dec 2020 | 17:42:40 19 Dec 2020 | 5 seconds |
| Examining read alignment evidence | 17:42:40 19 Dec 2020 | 17:42:47 19 Dec 2020 | 7 seconds |
| Polymorphism statistics | 17:42:47 19 Dec 2020 | 17:42:48 19 Dec 2020 | 1 second |
| Output | 17:42:48 19 Dec 2020 | 17:42:54 19 Dec 2020 | 6 seconds |
| Total | 1 minute 3 seconds | ||
