New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3140200	83 (0.950)	10 (0.120)	9/260	10.5	11.6%	coding (119/342 nt)	hybF	protein involved with the maturation of hydrogenases 1 and 2
	?	NC_000913_3_KO_pgi	= 3140206	76 (0.930)					coding (113/342 nt)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACACTGCGCATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGG  >  NC_000913_3_KO_pgi/3140052‑3140348                                                                                                                                                     |                                                                                                                                                     acacTGCGCATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTctcctc                                                                                                                                                     >  3:51336/1‑150 (MQ=255)    cTGCGCATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAAc                                                                                                                                                  <  2:906187/150‑1 (MQ=255)     tGCGCATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACg                                                                                                                                                 >  4:361406/1‑150 (MQ=255)       cgcATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCa                                                                                                                                               >  2:92903/1‑150 (MQ=255)        gcATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAg                                                                                                                                              <  1:791891/150‑1 (MQ=255)           tCGGGCTGGTGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGgag                                                                                                                                           <  2:150947/150‑1 (MQ=255)            cGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGgaga                                                                                                                                          <  1:383264/150‑1 (MQ=255)                cTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAgcgc                                                                                                                                      <  1:860092/150‑1 (MQ=255)                         ctcCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTCCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTc                                                                                                                             >  4:17995/1‑150 (MQ=255)                          tcCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTAGCGCCACCGTTCCGCGGCAGACAATTCCAAAACTCAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCCCCAATTTcc                                                                                                                            >  2:875950/1‑150 (MQ=255)                            caccacCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAg                                                                                                                          >  4:559142/1‑150 (MQ=255)                            caccacCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAg                                                                                                                          <  2:109401/150‑1 (MQ=255)                             accaccTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGc                                                                                                                         <  1:917860/150‑1 (MQ=255)                                  cTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCacac                                                                                                                    >  2:171877/1‑150 (MQ=255)                                    ggCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCCCTCCTCACCGCAGGAGACCGCGCCAATTTCCAGCCACAcgg                                                                                                                  >  4:511130/1‑150 (MQ=255)                                        gCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCGGACAATTTCAAAACTAAAACGGACGGCGCTctcctgc                                                                                                                                                    >  2:2896/1‑110 (MQ=255)                                        gCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCGGACAATTTCAAAACTAAAACGGACGGCGCTctcctgc                                                                                                                                                    <  1:2896/112‑3 (MQ=255)                                              ccAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGAcgcg                                                                                                        >  4:139241/1‑150 (MQ=255)                                              ccAGCACCAAGCCTGGGCGGGTTGATAGACGATATGTAAAGCGCACCCTGGCGCCACCGTTCCGTGGAAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGAAGAGCGCGCCAAGTTCCAGCCACACGGCGGTGAcgcg                                                                                                        >  2:820101/1‑150 (MQ=255)                                               cAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACgcgc                                                                                                       <  4:308442/150‑1 (MQ=255)                                               cAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACgcgc                                                                                                       >  4:540041/1‑150 (MQ=255)                                                         ccTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGt                                                                                             <  2:562957/150‑1 (MQ=255)                                                          cTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtg                                                                                            <  3:330959/150‑1 (MQ=255)                                                          cTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtg                                                                                            <  2:471896/150‑1 (MQ=255)                                                           tGGGCGGGTTTATAGTCGATATGTAAATCGCACCCTTGCGTGACCGTACTGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtgc                                                                                           >  2:91069/1‑150 (MQ=255)                                                           tGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtgc                                                                                           <  1:407035/150‑1 (MQ=255)                                                            gggCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtgct                                                                                          <  3:475140/150‑1 (MQ=255)                                                              gCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGtgctgc                                                                                        >  4:207005/1‑150 (MQ=255)                                                                  gTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCg                                                                                    >  2:857717/1‑150 (MQ=255)                                                                      ataGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTg                                                                                >  3:140/1‑150 (MQ=255)                                                                       taGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGc                                                                               <  1:857717/150‑1 (MQ=255)                                                                        aGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGcc                                                                              >  1:478328/1‑150 (MQ=255)                                                                         gACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCg                                                                             >  2:313556/1‑150 (MQ=255)                                                                         gACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGCGACGCGCTCACCATCGGGCTGCTCCGCCTGCCg                                                                             >  2:497342/1‑150 (MQ=255)                                                                               atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTTGATTTTGCGGCGCTCTCCTCAGCGCATGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGAt                                                                       <  3:290860/150‑1 (MQ=255)                                                                               atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGAt                                                                       <  1:923493/150‑1 (MQ=255)                                                                               atGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGAt                                                                       <  4:225238/150‑1 (MQ=255)                                                                                tGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATa                                                                      >  2:777169/1‑150 (MQ=255)                                                                                tGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATa                                                                      >  3:251522/1‑150 (MQ=255)                                                                                 gTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATaa                                                                     >  2:118299/1‑150 (MQ=255)                                                                                  tAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAt                                                                    >  1:667706/1‑150 (MQ=255)                                                                                  tAAATCACACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAt                                                                    >  2:23444/1‑150 (MQ=255)                                                                                     aTCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTCGGATAATTTc                                                                 >  2:561979/1‑150 (MQ=255)                                                                                       cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAgcgcgc                                                                                                                                    >  2:533031/1‑81 (MQ=255)                                                                                       cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAgcgcgc                                                                                                                                    >  2:532824/1‑81 (MQ=255)                                                                                       cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAgcgcgc                                                                                                                                    <  1:532824/81‑1 (MQ=255)                                                                                       cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAgcgcgc                                                                                                                                    <  1:533031/81‑1 (MQ=255)                                                                                       cGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCaa                                                               <  1:899806/150‑1 (MQ=255)                                                                                        gCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAGCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAAc                                                              <  2:655264/150‑1 (MQ=255)                                                                                         cACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACg                                                             <  4:528217/150‑1 (MQ=255)                                                                                            ccTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGgcg                                                          >  3:285034/1‑150 (MQ=255)                                                                                              ttGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTcc                                                                                                                            >  1:153662/1‑82 (MQ=255)                                                                                              ttGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTcc                                                                                                                            <  2:153662/82‑1 (MQ=255)                                                                                                  gcCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCACCGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTgg                                                    >  4:71457/1‑150 (MQ=255)                                                                                                  gcCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAg                                                                                                                          <  1:732150/80‑1 (MQ=255)                                                                                                  gcCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAg                                                                                                                          >  2:732150/1‑80 (MQ=255)                                                                                                  gcCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTgg                                                    >  2:276302/1‑150 (MQ=255)                                                                                                   ccACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTgg                                                                         <  4:223484/128‑1 (MQ=255)                                                                                                   ccACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTgg                                                                         >  3:223484/1‑128 (MQ=255)                                                                                                     aCCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCaa                                                 <  2:280932/150‑1 (MQ=255)                                                                                                        gTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAaga                                              >  3:123186/1‑150 (MQ=255)                                                                                                        gTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAaga                                              <  1:898247/150‑1 (MQ=255)                                                                                                             gTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACaa                                         <  3:540041/150‑1 (MQ=255)                                                                                                                gCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTc                                      <  4:251522/150‑1 (MQ=255)                                                                                                                 cAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGAAAACTCa                                     <  2:407605/150‑1 (MQ=255)                                                                                                                     cAATTTCAAAACTACAACGGACGGCGCTCTCCACAACGCAGGAGCGCGCGCCAATTTCCAGCCACACGACAGTGACGCGCTTAACATCGTGCTGCACCCCCTGCCGATGGATAATTCCAACGGCGCCCTGCCACACAGCCACCTCactcc                                 >  4:440040/1‑146 (MQ=255)                                                                                                                        tttCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCAttt                              <  2:96754/150‑1 (MQ=255)                                                                                                                         ttCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCAtttt                             >  3:592582/1‑150 (MQ=255)                                                                                                                         ttCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCAtttt                             >  2:823972/1‑150 (MQ=255)                                                                                                                          tCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGCGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGCCAACTCATGCATTTTc                            >  2:874542/1‑150 (MQ=255)                                                                                                                           cAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATCCATTTTCg                           >  2:824080/1‑150 (MQ=255)                                                                                                                            aaaaCTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGc                          <  1:23444/150‑1 (MQ=255)                                                                                                                            aaaaCTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGc                          <  1:765097/150‑1 (MQ=255)                                                                                                                                 tAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTc                     >  2:158152/1‑150 (MQ=255)                                                                                                                                    aaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                  >  1:791849/1‑150 (MQ=255)                                                                                                                                       ggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                    >  3:445248/1‑145 (MQ=255)                                                                                                                                       ggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                    <  4:445248/145‑1 (MQ=255)                                                                                                                                          cggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCa            <  1:118299/150‑1 (MQ=255)                                                                                                                                             cgcTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAc         >  3:402754/1‑150 (MQ=255)                                                                                                                                             cgcTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGAGCTTAACATAGTACTGCTACCCCTGCCGATGGATAATATCAAAGGCGCTATGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAc         <  1:91069/150‑1 (MQ=255)                                                                                                                                                 ctcctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagca     >  1:249887/1‑150 (MQ=255)                                                                                                                                                 ctcctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagca     <  2:751631/150‑1 (MQ=255)                                                                                                                                                    ctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAggg  <  1:777169/150‑1 (MQ=255)                                                                                                                                                    ctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAggg  >  1:278622/1‑150 (MQ=255)                                                                                                                                                    ctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAggg  >  3:346651/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ACACTGCGCATCGTGCTGATGAATCTCCACCACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGG  >  NC_000913_3_KO_pgi/3140052‑3140348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.