New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	3423376 =	96 (1.170)	17 (0.210)	10/274	8.2	16.9%	intergenic (+182/+47)	yhdZ/rrfF	putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
	?	NC_000913_3_KO_pgi	= 4213162	73 (0.900)					intergenic (+3/‑72)	rrfE/yjaA	5S ribosomal RNA of rrnE operon/stress‑induced protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGC  >  NC_000913_3_KO_pgi/3423228‑3423525                                                                                                                                                     |                                                                                                                                                      cTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCCAATCCGATCCAGGCGGATTTGTCCTACTCGGGAGAGCGTTCACCGACCAACAACAGAt                                                                                                                                                      >  1:1386090/1‑150 (MQ=14)    tCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATaaa                                                                                                                                                   <  1:1401230/150‑1 (MQ=255)     tggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATaaaa                                                                                                                                                  <  2:767508/149‑1 (MQ=255)          gggcTTTCGCTTGATTTGATGTCTCGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGGAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCTGTAGAGTGTTCACCGACAAACAACAGATAAAACaaaa                                                                                                                                             <  1:1436528/147‑1 (MQ=14)          gcccTTTCGCTTGATTTGATGTCCGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACaaaa                                                                                                                                             <  1:1199170/148‑1 (MQ=255)              tttCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGcc                                                                                                                                         <  2:1190260/150‑1 (MQ=255)                    ttgatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACACCAGATAAAACAAAAGGCCCAGTCt                                                                                                                                   >  2:51862/1‑150 (MQ=255)                      gatttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAACCTCCGGGCCGTTGCTTCGCAACGTTCACATCAGCTCTCGTCTTATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTc                                                                                                                                 <  2:1324976/150‑1 (MQ=14)                      gatttgatGGCTGGCAGTTTATGGCGGGCGGCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTc                                                                                                                                 <  1:619698/150‑1 (MQ=255)                       atttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGCCAAACAACAGATAAAACAAAAGGCCCCGACTTcc                                                                                                                                >  2:598797/1‑150 (MQ=255)                          tgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                                                                                                             >  1:887659/1‑150 (MQ=255)                              gTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCCCCTGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCCCCTGac                                                                                                                         >  2:319981/1‑149 (MQ=255)                               tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGTAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTgcgc                                                                                                                        >  1:968916/1‑150 (MQ=255)                               tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                                                                                                        >  1:745478/1‑150 (MQ=38)                               tCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                                                                                                        <  1:772178/150‑1 (MQ=39)                               tCTGGCAGTTTATGGCGGGCGCCCTGCCCCCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                                                                                                        <  1:511112/150‑1 (MQ=255)                                 tGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTTGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGCCCCAGTCTTCCGACTGAGCCt                                                                                                                      >  1:1275459/1‑150 (MQ=255)                                 tGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt                                                                                                                      >  1:839280/1‑150 (MQ=37)                                 tGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt                                                                                                                      >  1:113855/1‑150 (MQ=37)                                   gCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGa                                                                                                                                                       <  1:1291747/115‑1 (MQ=38)                                   gCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGa                                                                                                                                                       >  2:1291747/1‑115 (MQ=37)                                    cAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtttt                                                                                                                   <  2:427787/150‑1 (MQ=38)                                    cAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtttt                                                                                                                   <  1:1125804/150‑1 (MQ=40)                                    cAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtttt                                                                                                                   <  2:1342342/150‑1 (MQ=38)                                    cAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtttt                                                                                                                   <  1:503300/150‑1 (MQ=255)                                     aGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTg                                                                                                                  >  1:947493/1‑150 (MQ=38)                                     aGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTg                                                                                                                  <  2:991191/150‑1 (MQ=40)                                       tttATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtt                                                                                                                <  1:488033/150‑1 (MQ=255)                                       tttATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtt                                                                                                                <  2:714439/150‑1 (MQ=40)                                        ttATGGCGGGCGTCCTGTCCGCCACCATCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTACCGGCGGATTTGTCCTACTCGGCAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGttt                                                                                                               <  2:51625/150‑1 (MQ=255)                                            ggcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAAATAAAACAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTAtc                                                                                                           >  2:899739/1‑149 (MQ=14)                                            ggcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCCCAACGTTCCACTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAtt                                                                                                           <  1:430472/150‑1 (MQ=255)                                               gggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                        <  1:306162/150‑1 (MQ=255)                                                ggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTTAAATTCGCTTCCGGCGGATTTGTCCTACTCGGGAGGGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAt                                                                                                       <  2:1324584/150‑1 (MQ=255)                                                ggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCATATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAt                                                                                                       >  1:1155310/1‑150 (MQ=255)                                                ggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAt                                                                                                       <  2:113855/150‑1 (MQ=40)                                                  cgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGt                                                                                                     <  2:1379264/150‑1 (MQ=255)                                                    tCCTGCCCGCCACCCTCCGGGGCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                   >  2:1281429/1‑150 (MQ=255)                                                    tCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                   <  1:1374387/150‑1 (MQ=255)                                                        gCCCGCCACCCTCCGGGTCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCa                                                                                               <  2:1087740/150‑1 (MQ=255)                                                          ccGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCTCTCCCGGCGGATTTGTCCTACTCGGGACAGTGTTCACCGACAAACAACAGATAAAACACAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTggtcgt                                                                                             >  2:744120/1‑146 (MQ=255)                                                                    ccGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGGCTTCCGCCTGGGCTGGTTTTTGTATTTGATGTCTGGCAGTTCCCTActct                                                                                   <  1:1085277/150‑1 (MQ=14)                                                                      gggCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCg                                                                                 <  2:895769/150‑1 (MQ=255)                                                                           gTTGCTTCGGAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATgg                                                                            <  2:98018/150‑1 (MQ=255)                                                                           gTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGCTGTCTGGCAGTTCCCTACTCTCGCATgg                                                                            >  2:564403/1‑150 (MQ=255)                                                                           gTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGTCCGTTCCCTACTCTCGCATgg                                                                            >  2:1073689/1‑150 (MQ=255)                                                                            ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGATAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATggg                                                                           <  2:1289910/150‑1 (MQ=255)                                                                              gCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGga                                                                         <  2:947493/150‑1 (MQ=255)                                                                                ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCCGTCACCTCACTGATCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                       <  1:1146457/150‑1 (MQ=14)                                                                                ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                       >  2:463200/1‑150 (MQ=255)                                                                                ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                       <  1:542578/150‑1 (MQ=255)                                                                                ttCGCAACGTTCAAATCCGCTCCCGGCCGATTTGTACTACTCGGGAGAGTGTTCACCGACAAACAACAGCTAAAACAAAAGGCCCAGACTTCCGACCGTGCCTATTGTTTTAGTTGATGTCTTGACGATCCCTACTCTCGCATggggcca                                                                       >  2:918622/1‑147 (MQ=14)                                                                                   gCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCACGCATGGGGAGAccc                                                                    >  2:851727/1‑150 (MQ=255)                                                                                     aaCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCGCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGAATAGGGAGAccccc                                                                  >  2:1223933/1‑149 (MQ=255)                                                                                        gTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                               >  1:1080815/1‑150 (MQ=255)                                                                                        gTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTACCCCCTGTCGCATGGGGAGCCCCCacac                                                               >  2:742033/1‑150 (MQ=14)                                                                                             aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGttt                                                                                                               <  2:590296/97‑1 (MQ=255)                                                                                             aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGttt                                                                                                               >  1:590296/1‑97 (MQ=255)                                                                                             aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCa                                                          >  1:980724/1‑150 (MQ=255)                                                                                              aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAt                                                         >  2:878894/1‑150 (MQ=255)                                                                                              aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAt                                                         <  1:1365976/150‑1 (MQ=255)                                                                                                 cGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCgg                                                      >  1:379676/1‑150 (MQ=255)                                                                                                    tCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGAc                                                                      <  1:836127/131‑1 (MQ=255)                                                                                                    tCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGAc                                                                      >  2:836127/1‑131 (MQ=255)                                                                                                          gggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                             <  2:745478/149‑1 (MQ=255)                                                                                                                tGTCCTACTCGGTAGAGTGTTCACTGACAAACAACATATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTTTGGCAGTTCCCTACTCTCGCATGGGGAGACCTCACACTACCATCGGCGCTACGGCGTTTCa                                       <  1:1227181/150‑1 (MQ=14)                                                                                                                tGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                       <  2:1099330/150‑1 (MQ=255)                                                                                                                  tCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACt                                     <  2:116992/150‑1 (MQ=255)                                                                                                                   ccTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                    >  2:1048472/1‑150 (MQ=255)                                                                                                                    cTACTCGGGAGAGTGTTCACCGACAAACAACAGCTAAAACAACAGCCCCCGTCTTACGACTGAGACTTTTGTTATTTTTGATCAACCGCAGTGCTCTACTTTCGCATTCTTGTACCCCACACTACCACCTGCGCTCCGCCGTTTCACtcc                                   >  2:721055/1‑148 (MQ=255)                                                                                                                     tACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTTGCGTGGGGAGACCCCACACTACCATCGGCGTTACGGCGTGTCtcttct                                  >  2:61709/1‑150 (MQ=14)                                                                                                                     tACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTCCGGCGTTTCACTTCt                                  >  1:306503/1‑150 (MQ=255)                                                                                                                     tACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGCCGCTACGGCGTTTCCCTTCt                                  >  2:939979/1‑150 (MQ=255)                                                                                                                       cTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                <  2:1313052/150‑1 (MQ=255)                                                                                                                        tCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACCCTACCATCGCCGCTACGGCGTTTCGCTTCTGat                               >  2:814312/1‑149 (MQ=255)                                                                                                                          gggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                             >  2:514624/1‑150 (MQ=255)                                                                                                                             agagTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                          <  2:760758/150‑1 (MQ=255)                                                                                                                               agTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCa                        <  2:887659/150‑1 (MQ=255)                                                                                                                                      aCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATgg                                                                            <  1:38772/91‑1 (MQ=255)                                                                                                                                      aCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATgg                                                                            >  2:38772/1‑91 (MQ=255)                                                                                                                                      aCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTc                 >  1:940162/1‑150 (MQ=255)                                                                                                                                      aCCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTc                 >  2:769274/1‑150 (MQ=40)                                                                                                                                      aCCGACAAACAACAGATAAAACAAAAGACCCGTTCTCCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCTGTTCCCTACTCTCGCATGGGGAGACCCCAAAAAACCATCGGCGCTCCGGCGGTTCCCTTCTGAATTGGGCATGGGGTc                 >  1:728125/1‑150 (MQ=14)                                                                                                                                        cGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg               <  2:1080815/150‑1 (MQ=40)                                                                                                                                          acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt             <  1:742033/150‑1 (MQ=255)                                                                                                                                            aaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg           <  1:598797/150‑1 (MQ=40)                                                                                                                                             aacaacAGATAAAACAAAAGGCCGAGTCTGGCGACTGAGCTTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGAGCTCAGAAGAGCATCGGCGCGAGGGAGGGTCACTTATACGGTGGGCATCGGGGGAGGTggg          >  2:756551/1‑150 (MQ=2)                                                                                                                                              acaacaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa         >  1:253421/1‑150 (MQ=255)                                                                                                                                                 acaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca      <  1:1281429/150‑1 (MQ=255)                                                                                                                                                 acaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac        <  2:578196/148‑1 (MQ=255)                                                                                                                                                 acaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac        >  1:578196/1‑148 (MQ=255)                                                                                                                                                  caGATAAACCAAAACCCCCCGTCTTCCTACTGAGCCTTTTGTTTTATTACATGTCTGGCAGTTCCCTGCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac     <  1:198125/150‑1 (MQ=14)                                                                                                                                                  caGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                    >  2:1295826/1‑135 (MQ=255)                                                                                                                                                  caGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGCCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac     >  2:1279074/1‑150 (MQ=255)                                                                                                                                                  caGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTCATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                    <  1:1295826/135‑1 (MQ=255)                                                                                                                                                    gATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg   >  2:1142873/1‑150 (MQ=255)                                                                                                                                                     aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                         >  2:278302/1‑111 (MQ=255)                                                                                                                                                     aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                         <  1:278302/111‑1 (MQ=255)                                                                                                                                                     aTAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc  <  1:905597/150‑1 (MQ=39)                                                                                                                                                     |                                                                                                                                                      CTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGC  >  NC_000913_3_KO_pgi/3423228‑3423525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.